TY - JOUR
T1 - A Functional Variant of PTPN22 Confers Risk for Vogt-Koyanagi-Harada Syndrome but Not for Ankylosing Spondylitis
AU - Zhang, Qi
AU - Qi, Jian
AU - Hou, Shengping
AU - Du, Liping
AU - Yu, Hongsong
AU - Cao, Qingfeng
AU - Zhou, Yan
AU - Liao, Dan
AU - Kijlstra, Aize
AU - Yang, Peizeng
PY - 2014/5/9
Y1 - 2014/5/9
N2 - Background: Protein tyrosine phosphatase non-receptor 22 (PTPN22) is a key negative regulator of T lymphocytes and has emerged as an important candidate susceptibility factor for a number of immune-related diseases. This study aimed to examine the predisposition of PTPN22 SNPs to Vogt-Koyanagi-Harada (VKH) syndrome and acute anterior uveitis (AAU) associated with ankylosing spondylitis (AS). Methods: A total of 1005 VKH syndrome, 302 AAU(+) AS(+) patients and 2010 normal controls among the Chinese Han population were enrolled in the study. Genotyping, PTPN22 expression, cell proliferation, cytokine production and cell activation were examined by PCR-RFLP, Real-time PCR, CCK8, ELISA and Flow cytometry. Results: The results showed significantly increased frequencies of the rs2488457 CC genotype and C allele but a decreased frequency of the GG genotype in VKH syndrome patients (P-Bonferroni correction (P-c) = 3.47x10(-7), OR = 1.54; P-c = 3.83 x 10(-8), OR = 1.40; P-c = 6.35x10(-4), OR = 0.62; respectively). No significant association of the tested SNPs with AAU(+) AS(+) patients was observed. Functional studies showed a decreased PTPN22 expression, impaired cell proliferation and lower production of IL-10 in rs2488457 CC cases compared to GG cases (P-c = 0.009, P-c = 0.015 and P-c = 0.048 respectively). No significant association was observed concerning T cell activation and rs2488457 genotype. Conclusions: The study showed that a functional variant of PTPN22 confers risk for VKH syndrome but not for AAU(+) AS(+) in a Chinese Han population, which may be due to a modulation of the PTPN22 expression, PBMC proliferation and IL-10 production.
AB - Background: Protein tyrosine phosphatase non-receptor 22 (PTPN22) is a key negative regulator of T lymphocytes and has emerged as an important candidate susceptibility factor for a number of immune-related diseases. This study aimed to examine the predisposition of PTPN22 SNPs to Vogt-Koyanagi-Harada (VKH) syndrome and acute anterior uveitis (AAU) associated with ankylosing spondylitis (AS). Methods: A total of 1005 VKH syndrome, 302 AAU(+) AS(+) patients and 2010 normal controls among the Chinese Han population were enrolled in the study. Genotyping, PTPN22 expression, cell proliferation, cytokine production and cell activation were examined by PCR-RFLP, Real-time PCR, CCK8, ELISA and Flow cytometry. Results: The results showed significantly increased frequencies of the rs2488457 CC genotype and C allele but a decreased frequency of the GG genotype in VKH syndrome patients (P-Bonferroni correction (P-c) = 3.47x10(-7), OR = 1.54; P-c = 3.83 x 10(-8), OR = 1.40; P-c = 6.35x10(-4), OR = 0.62; respectively). No significant association of the tested SNPs with AAU(+) AS(+) patients was observed. Functional studies showed a decreased PTPN22 expression, impaired cell proliferation and lower production of IL-10 in rs2488457 CC cases compared to GG cases (P-c = 0.009, P-c = 0.015 and P-c = 0.048 respectively). No significant association was observed concerning T cell activation and rs2488457 genotype. Conclusions: The study showed that a functional variant of PTPN22 confers risk for VKH syndrome but not for AAU(+) AS(+) in a Chinese Han population, which may be due to a modulation of the PTPN22 expression, PBMC proliferation and IL-10 production.
U2 - 10.1371/journal.pone.0096943
DO - 10.1371/journal.pone.0096943
M3 - Article
C2 - 24816862
SN - 1932-6203
VL - 9
JO - PLOS ONE
JF - PLOS ONE
IS - 5
M1 - e96943
ER -