Within-person reproducibility and sensitivity to dietary change of C15: 0 and C17:0 levels in dried blood spots: Data from the European Food4Me Study

Viviana Albani, Carlos Celis-Morales, Clare B. O'Donovan, Marianne C. Walsh, Clara Woolhead, Hannah Forster, Rosalind Fallaize, Anna L. Macready, Cyril F. M. Marsaux, Santiago Navas-Carretero, Rodrigo San-Cristobal, Silvia Kolossa, Christina Mavrogianni, Christina P. Lambrinou, George Moschonis, Magdalena Godlewska, Agnieszka Surwillo, Iwona Traczyk, Thomas E. Gundersen, Christian A. DrevonHannelore Daniel, Yannis Manios, J. Alfredo Martinez, Wim H. M. Saris, Julie A. Lovegrove, Michael J. Gibney, Eileen R. Gibney, John C. Mathers, Ashley J. Adamson, Lorraine Brennan*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Scope: Previous work highlighted the potential of odd-chain length saturated fatty acids as potential markers of dairy intake. The aim of this study was to assess the reproducibility of these biomarkers and their sensitivity to changes in dairy intake.

Methods and results: Fatty acid profiles and dietary intakes from food frequency questionnaires (FFQs) were measured three times over six months in the Food4Me Study. Reproducibility was explored through intra-class correlation coefficients (ICCs) and within-subject coefficients of variation (WCV). Sensitivity to changes in diet was examined using regression analysis. C15:0 blood levels showed high correlation over time (ICC: 0.62, 95% CI: 0.57, 0.68), however, the ICC for C17:0 was much lower (ICC: 0.32, 95% CI: 0.28, 0.46). The WCV for C15:0 was 16.6% and that for C17:0 was 14.6%. There were significant associations between changes in intakes of total dairy, high-fat dairy, cheese and butter and C15:0; and change in intakes of high-fat dairy and cream and C17:0.

Conclusion: Results provide evidence of reproducibility of C15:0 levels over time and sensitivity to change in intake of high-fat dairy products with results comparable to the well-established biomarker of fish intake (EPA+DHA).

Original languageEnglish
Article number1700142
Number of pages9
JournalMolecular Nutrition & Food Research
Volume61
Issue number10
DOIs
Publication statusPublished - Oct 2017

Keywords

  • Biomarkers
  • Dairy intake
  • Dried blood spots
  • Heptadecanoic acid
  • Intra-class correlation
  • Pentadecanoic acid
  • DAIRY PRODUCT INTAKE
  • CHAIN FATTY-ACIDS
  • ODD-CHAIN
  • ADIPOSE-TISSUE
  • WHOLE-BLOOD
  • SERUM
  • ASSOCIATION
  • COHORT
  • BIOMARKER
  • PLASMA

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