Whole Blood Based Multiparameter Assessment of Thrombus Formation in Standard Microfluidic Devices to Proxy In Vivo Haemostasis and Thrombosis

Isabella Provenzale*, Sanne L. N. Brouns, Paola E. J. van der Meijden, Frauke Swieringa, Johan W. M. Heemskerk*

*Corresponding author for this work

Research output: Contribution to journal(Systematic) Review article peer-review

5 Citations (Web of Science)

Abstract

Microfluidic assays are versatile tests which, using only small amounts of blood, enable high throughput analyses of platelet function in several minutes. In combination with fluorescence microscopy, these flow tests allow real-time visualisation of platelet activation with the possibility of examining combinatorial effects of wall shear rate, coagulation and modulation by endothelial cells. In particular, the ability to use blood and blood cells from healthy subjects or patients makes this technology promising, both for research and (pre)clinical diagnostic purposes. In the present review, we describe how microfluidic devices are used to assess the roles of platelets in thrombosis and haemostasis. We place emphasis on technical aspects and on experimental designs that make the concept of "blood-vessel-component-on-a-chip" an attractive, rapidly developing technology for the study of the complex biological processes of blood coagulability in the presence of flow.

Original languageEnglish
Article number787
Number of pages17
JournalMicromachines
Volume10
Issue number11
DOIs
Publication statusPublished - Nov 2019

Keywords

  • collagen
  • platelet
  • thrombus
  • VON-WILLEBRAND-FACTOR
  • INDUCED PLATELET-AGGREGATION
  • FLOW-BASED ASSAYS
  • TISSUE FACTOR
  • ENDOTHELIAL-CELLS
  • GLOBAL ASSESSMENT
  • EX-VIVO
  • ADHESION
  • ACTIVATION
  • MODEL

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