TY - JOUR
T1 - Versatile immobilization of mimicking peptides on additively manufactured functionalized a-amino acid based poly(ester amide)s
AU - Ansari, Vahid
AU - Massonnet, Philippe
AU - Weng, Hongjuan
AU - Kalogeropoulou, Maria
AU - Heeren, Ron M.A.
AU - Harings, Jules A.W.
AU - Moroni, Lorenzo
AU - Bernaerts, Katrien V.
N1 - Funding Information:
The current work has been performed as part of the Program Additive Manufacturing of the Brightlands Materials Center (BMC). The authors acknowledge BMC for supporting this work.
Publisher Copyright:
© 2024 The Authors
PY - 2025/1/6
Y1 - 2025/1/6
N2 - a-Amino acid based polyester amides (AA-PEAs) exhibit remarkable properties, including biocompatibility, biodegradability, flexibility, thermal stability, and mechanical integrity. The incorporation of a-amino acids enhances cytocompatibility, hydrogen bonding, and favorable cell-polymer interactions, making AA-PEAs appealing for biomedical applications, notably tissue engineering. However, addressing complex tissue regeneration requires additional enhancements. Introducing biologically instructive factors, like growth factors and peptides, becomes essential to facilitate cell growth, proliferation, and differentiation. This study explores a-amino acid based functionalized polyester amides (AA-FPEAs) for their potential in tissue engineering, focusing on their underexplored role as thermoplastic resources for fused deposition modeling (FDM). Novel AA-FPEAs with alkyne moieties were synthesized and additively manufactured via FDM, highlighting their structure–property correlation. Employing a facile copper-free click chemistry strategy, we successfully attached a CGRGDS mimicking peptide to AA-FPEAs using UV light and a photoinitiator with water as a solvent. UV–Vis analysis confirmed the feasibility of the click reaction, and TOF-SIMS analysis verified CGRGDS attachment on AA-FPEA films and AM scaffolds. In vitro evaluation further demonstrated that AA-FPEAs support cell growth and proliferation, highlighting their biocompatibility. These findings underscore the potential of AA-FPEAs as versatile functionalized biomaterials for tissue engineering applications.
AB - a-Amino acid based polyester amides (AA-PEAs) exhibit remarkable properties, including biocompatibility, biodegradability, flexibility, thermal stability, and mechanical integrity. The incorporation of a-amino acids enhances cytocompatibility, hydrogen bonding, and favorable cell-polymer interactions, making AA-PEAs appealing for biomedical applications, notably tissue engineering. However, addressing complex tissue regeneration requires additional enhancements. Introducing biologically instructive factors, like growth factors and peptides, becomes essential to facilitate cell growth, proliferation, and differentiation. This study explores a-amino acid based functionalized polyester amides (AA-FPEAs) for their potential in tissue engineering, focusing on their underexplored role as thermoplastic resources for fused deposition modeling (FDM). Novel AA-FPEAs with alkyne moieties were synthesized and additively manufactured via FDM, highlighting their structure–property correlation. Employing a facile copper-free click chemistry strategy, we successfully attached a CGRGDS mimicking peptide to AA-FPEAs using UV light and a photoinitiator with water as a solvent. UV–Vis analysis confirmed the feasibility of the click reaction, and TOF-SIMS analysis verified CGRGDS attachment on AA-FPEA films and AM scaffolds. In vitro evaluation further demonstrated that AA-FPEAs support cell growth and proliferation, highlighting their biocompatibility. These findings underscore the potential of AA-FPEAs as versatile functionalized biomaterials for tissue engineering applications.
KW - Additive manufacturing
KW - Biomedical application
KW - Biomimetic peptide immobilization
KW - Copper-free click chemistry
KW - Functionalized poly(ester amide)
U2 - 10.1016/j.eurpolymj.2024.113593
DO - 10.1016/j.eurpolymj.2024.113593
M3 - Article
SN - 0014-3057
VL - 222
JO - European Polymer Journal
JF - European Polymer Journal
M1 - 113593
ER -