VEGF-A-induced chemotaxis of CD16+monocytes is decreased secondary to lower VEGFR-1 expression

Frauke S. Czepluch, Serve Olieslagers, Ruud van Hulten, Stefan A. Voo, Johannes Waltenberger*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review


Background: Monocyte recruitment into the vessel wall is a crucial initial step in vascular repair, arteriogenesis and atherogenesis. Two distinct human monocyte subpopulations can be classified according to their CD14/16 surface expression, namely CD14++CD16-monocytes (CD16 mo) and CD14+CD16+ monocytes (CD16+mo). We investigated different functional properties of the two monocyte subsets. Methods: CD16 /CD16+mo were isolated from human blood by an immunological selection. We assessed monocyte chemokinesis, chemotaxis, adhesion and Vascular-Endothelial Growth Factor (VEGF) receptor expression. Furthermore, generation of reactive oxygen species (ROS) as well as expression of antioxidant enzymes was investigated. Results: Chemokinesis of CD16+mo was decreased compared to CD16 mo (p
Original languageEnglish
Pages (from-to)331-338
Issue number2
Publication statusPublished - Apr 2011


  • Monocyte
  • CD16
  • Migration
  • VEGFR-1
  • VEGF-A
  • PIGF-1
  • Antioxidant enzyme

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