VEGF-A-induced chemotaxis of CD16+monocytes is decreased secondary to lower VEGFR-1 expression

Frauke S. Czepluch; Serve Olieslagers; Ruud van Hulten; Stefan A. Voo; Johannes Waltenberger

doi:10.1016/j.atherosclerosis.2011.01.004

VEGF-A-induced chemotaxis of CD16+monocytes is decreased secondary to lower VEGFR-1 expression

Frauke S. Czepluch, Serve Olieslagers, Ruud van Hulten, Stefan A. Voo, Johannes Waltenberger^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Background: Monocyte recruitment into the vessel wall is a crucial initial step in vascular repair, arteriogenesis and atherogenesis. Two distinct human monocyte subpopulations can be classified according to their CD14/16 surface expression, namely CD14++CD16-monocytes (CD16 mo) and CD14+CD16+ monocytes (CD16+mo). We investigated different functional properties of the two monocyte subsets. Methods: CD16 /CD16+mo were isolated from human blood by an immunological selection. We assessed monocyte chemokinesis, chemotaxis, adhesion and Vascular-Endothelial Growth Factor (VEGF) receptor expression. Furthermore, generation of reactive oxygen species (ROS) as well as expression of antioxidant enzymes was investigated. Results: Chemokinesis of CD16+mo was decreased compared to CD16 mo (p

Original language	English
Pages (from-to)	331-338
Journal	Atherosclerosis
Volume	215
Issue number	2
DOIs	https://doi.org/10.1016/j.atherosclerosis.2011.01.004
Publication status	Published - Apr 2011

Keywords

Monocyte
CD16
Migration
VEGFR-1
VEGF-A
PIGF-1
Antioxidant enzyme

Access to Document

10.1016/j.atherosclerosis.2011.01.004

Cite this

@article{02c66746ae02472bb1c9af2f5ec96824,

title = "VEGF-A-induced chemotaxis of CD16+monocytes is decreased secondary to lower VEGFR-1 expression",

abstract = "Background: Monocyte recruitment into the vessel wall is a crucial initial step in vascular repair, arteriogenesis and atherogenesis. Two distinct human monocyte subpopulations can be classified according to their CD14/16 surface expression, namely CD14++CD16-monocytes (CD16 mo) and CD14+CD16+ monocytes (CD16+mo). We investigated different functional properties of the two monocyte subsets. Methods: CD16 /CD16+mo were isolated from human blood by an immunological selection. We assessed monocyte chemokinesis, chemotaxis, adhesion and Vascular-Endothelial Growth Factor (VEGF) receptor expression. Furthermore, generation of reactive oxygen species (ROS) as well as expression of antioxidant enzymes was investigated. Results: Chemokinesis of CD16+mo was decreased compared to CD16 mo (p ",

keywords = "Monocyte, CD16, Migration, VEGFR-1, VEGF-A, PIGF-1, Antioxidant enzyme",

author = "Czepluch, {Frauke S.} and Serve Olieslagers and {van Hulten}, Ruud and Voo, {Stefan A.} and Johannes Waltenberger",

year = "2011",

month = apr,

doi = "10.1016/j.atherosclerosis.2011.01.004",

language = "English",

volume = "215",

pages = "331--338",

journal = "Atherosclerosis",

issn = "0021-9150",

publisher = "Elsevier Ireland Ltd",

number = "2",

}

TY - JOUR

T1 - VEGF-A-induced chemotaxis of CD16+monocytes is decreased secondary to lower VEGFR-1 expression

AU - Czepluch, Frauke S.

AU - Olieslagers, Serve

AU - van Hulten, Ruud

AU - Voo, Stefan A.

AU - Waltenberger, Johannes

PY - 2011/4

Y1 - 2011/4

N2 - Background: Monocyte recruitment into the vessel wall is a crucial initial step in vascular repair, arteriogenesis and atherogenesis. Two distinct human monocyte subpopulations can be classified according to their CD14/16 surface expression, namely CD14++CD16-monocytes (CD16 mo) and CD14+CD16+ monocytes (CD16+mo). We investigated different functional properties of the two monocyte subsets. Methods: CD16 /CD16+mo were isolated from human blood by an immunological selection. We assessed monocyte chemokinesis, chemotaxis, adhesion and Vascular-Endothelial Growth Factor (VEGF) receptor expression. Furthermore, generation of reactive oxygen species (ROS) as well as expression of antioxidant enzymes was investigated. Results: Chemokinesis of CD16+mo was decreased compared to CD16 mo (p

AB - Background: Monocyte recruitment into the vessel wall is a crucial initial step in vascular repair, arteriogenesis and atherogenesis. Two distinct human monocyte subpopulations can be classified according to their CD14/16 surface expression, namely CD14++CD16-monocytes (CD16 mo) and CD14+CD16+ monocytes (CD16+mo). We investigated different functional properties of the two monocyte subsets. Methods: CD16 /CD16+mo were isolated from human blood by an immunological selection. We assessed monocyte chemokinesis, chemotaxis, adhesion and Vascular-Endothelial Growth Factor (VEGF) receptor expression. Furthermore, generation of reactive oxygen species (ROS) as well as expression of antioxidant enzymes was investigated. Results: Chemokinesis of CD16+mo was decreased compared to CD16 mo (p

KW - Monocyte

KW - CD16

KW - Migration

KW - VEGFR-1

KW - VEGF-A

KW - PIGF-1

KW - Antioxidant enzyme

U2 - 10.1016/j.atherosclerosis.2011.01.004

DO - 10.1016/j.atherosclerosis.2011.01.004

M3 - Article

C2 - 21324460

SN - 0021-9150

VL - 215

SP - 331

EP - 338

JO - Atherosclerosis

JF - Atherosclerosis

IS - 2

ER -