Variability of core microbiota in newly diagnosed treatment-naive paediatric inflammatory bowel disease patients

T. G. J. de Meij*, E. F. J. de Groot, C. F. W. Peeters, N. K. H. de Boer, C. M. F. Kneepkens, A. Eck, M. A. Benninga, P. H. M. Savelkoul, A. A. van Bodegraven, A. E. Budding

*Corresponding author for this work

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Abstract

Background & aims

Intestinal microbiota is considered to play a crucial role in the aetiology of inflammatory bowel disease (IBD). We aimed to describe faecal microbiota composition and dynamics in a large cohort of children with de novo (naive) IBD, in comparison to healthy paediatric controls (HC).

Methods

In this prospective study, performed at two tertiary centres, faecal samples from newly diagnosed, treatment-naive paediatric IBD patients were collected prior to bowel cleansing for colonoscopy (t0) and 1, 3 and 6 weeks and 3 months after initiation of therapy. The microbial profiles of Crohn's disease (CD) and Ulcerative colitis (UC) patients were compared with HC and linked to therapeutic response. Microbiota composition was analysed by IS-pro technology.

Results

Microbial profiles of 104 new IBD-patients (63 CD, 41 UC, median age 14.0 years) were compared to 61 HC (median 7.8 years). IBD was mainly characterised by decreased abundance of Alistipes finegoldii and Alistipes putredinis, which characterize a healthy state microbial core. The classifier including these core species as predictors achieved an AUC of the ROC curve of .87. Core bacteria tended to regain abundance during treatment, but did not reach healthy levels.

Conclusion

Faecal microbiota profiles of children with de novo CD and UC can be discriminated from HC with high accuracy, mainly driven by a decreased abundance of species shaping the microbial core in the healthy state. Paediatric IBD can therefore be characterized by decreased abundance of certain bacterial species reflecting the healthy state rather than by the introduction of pathogens.

Original languageEnglish
Article number0197649
Number of pages20
JournalPLOS ONE
Volume13
Issue number8
DOIs
Publication statusPublished - 13 Aug 2018

Keywords

  • CROHNS-DISEASE
  • GUT MICROBIOTA
  • INTESTINAL MICROBIOTA
  • ENTERAL NUTRITION
  • FECAL MICROBIOTA
  • RISING INCIDENCE
  • CHILDREN
  • PATHOGENESIS
  • IMPROVEMENT
  • MODULATION

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