Validity and reproducibility of VO(2)max testing in a respiration chamber

J.P.D. Kleinloog*, S.P.G.A. van Laar, P.F.M. Schoffelen, G. Plasqui

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review


The aim of this study was to investigate whether VO(2)max can be accurately measured in a respiration chamber. Thirty participants aged 23.4 +/- 3.9 years with a wide range in VO(2)max were included. Participants performed four incremental cycle ergometer tests (VO(2)max) with a minimum of 5 days between tests. These tests consisted of one familiarization test with face mask, followed by two VO(2)max tests in the respiration chamber and one test with face mask in randomized order. Oxygen consumption and CO2 production were measured continuously using Omnical (Maastricht University, the Netherlands) gas analysis system. The mean VO(2)max was 3634 +/- 766 ml, which resulted in mean VO(2)max per lean body mass of 60.8 +/- 8.0 ml/kg. Repeated respiration chamber tests showed a high concordance, and no significant differences were detected between tests (Lin's concordance correlation coefficient (Rc) = 0.99; increment 70 +/- 302 ml/min; p = .38). There was high concordance between the mean VO(2)max from both respiration chamber tests and the mean face mask tests, and no significant difference (Rc = 0.99; increment 41 +/- 173 ml/min; p = .22) was observed. The Bland-Altman plots showed no proportional bias between different tests. In conclusion, the respiration chamber has been found to be a valid and reproducible method for measuring VO(2)max. New research opportunities are possible in the respiration chamber, such as maximal exercise testing during 24-hour measurements.
Original languageEnglish
Pages (from-to)1259-1267
Number of pages9
JournalScandinavian Journal of Medicine & Science in Sports
Issue number6
Publication statusPublished - 1 Jun 2021


  • cycle ergometer
  • exercise
  • maximal oxygen consumption
  • Omnical
  • respiration chamber
  • validity

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