Validation of an isotope dilution gas chromatography-mass spectrometry method for analysis of 7-oxygenated campesterol and sitosterol in human serum

Constanze Husche; Oliver Weingaertner; Hanna Pettersson; Tim Vanmierlo; Michael Boehm; Ulrich Laufs; Dieter Lütjohann

doi:10.1016/j.chemphyslip.2011.04.009

Validation of an isotope dilution gas chromatography-mass spectrometry method for analysis of 7-oxygenated campesterol and sitosterol in human serum

Constanze Husche, Oliver Weingaertner, Hanna Pettersson, Tim Vanmierlo, Michael Boehm, Ulrich Laufs, Dieter Lütjohann^*

^*Corresponding author for this work

MHeNs School for Mental Health and Neuroscience

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

High dose daily intake of plant sterols decreases the uptake of cholesterol in the intestine by competitive mechanisms and thus leads to reduced serum levels of total and LDL-cholesterol. By this, the commercialization of plant sterol enriched 'functional food' products is rapidly increasing. Subjects using these kinds of diet present a duplication of their serum plant sterol levels after long-term intake. In analogy to cholesterol, plant sterols such as campesterol and sitosterol can be oxidized to oxyphytosterols and these may counteract the primary anti-atherosclerotic action of cholesterol lowering. In order to investigate the whole spectrum of the consequences following high plant sterol intake a highly sensitive and specific isotope dilution gas chromatography-mass spectrometry method for the analysis of 7-oxygenated campesterol/sitosterol in trace amounts in human serum is presented in this paper. The validation was based on limits for detection and quantification, recovery, precision and minimization of autoxidation during work-up. Our results show an overall coefficient of variation

Original language	English
Pages (from-to)	425-431
Journal	Chemistry and Physics of Lipids
Volume	164
Issue number	6
DOIs	https://doi.org/10.1016/j.chemphyslip.2011.04.009
Publication status	Published - Sept 2011

Keywords

Plant sterol
Oxyphytosterol
Low-density lipoprotein
Gas chromatograpy-mass spectrometry
Atherosclerosis
Cardiovascular disease

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10.1016/j.chemphyslip.2011.04.009

Cite this

@article{0187f171ec4f4bd996906524712fa862,

title = "Validation of an isotope dilution gas chromatography-mass spectrometry method for analysis of 7-oxygenated campesterol and sitosterol in human serum",

abstract = "High dose daily intake of plant sterols decreases the uptake of cholesterol in the intestine by competitive mechanisms and thus leads to reduced serum levels of total and LDL-cholesterol. By this, the commercialization of plant sterol enriched 'functional food' products is rapidly increasing. Subjects using these kinds of diet present a duplication of their serum plant sterol levels after long-term intake. In analogy to cholesterol, plant sterols such as campesterol and sitosterol can be oxidized to oxyphytosterols and these may counteract the primary anti-atherosclerotic action of cholesterol lowering. In order to investigate the whole spectrum of the consequences following high plant sterol intake a highly sensitive and specific isotope dilution gas chromatography-mass spectrometry method for the analysis of 7-oxygenated campesterol/sitosterol in trace amounts in human serum is presented in this paper. The validation was based on limits for detection and quantification, recovery, precision and minimization of autoxidation during work-up. Our results show an overall coefficient of variation ",

keywords = "Plant sterol, Oxyphytosterol, Low-density lipoprotein, Gas chromatograpy-mass spectrometry, Atherosclerosis, Cardiovascular disease",

author = "Constanze Husche and Oliver Weingaertner and Hanna Pettersson and Tim Vanmierlo and Michael Boehm and Ulrich Laufs and Dieter L{\"u}tjohann",

year = "2011",

month = sep,

doi = "10.1016/j.chemphyslip.2011.04.009",

language = "English",

volume = "164",

pages = "425--431",

journal = "Chemistry and Physics of Lipids",

issn = "0009-3084",

publisher = "Elsevier Ireland Ltd",

number = "6",

}

Validation of an isotope dilution gas chromatography-mass spectrometry method for analysis of 7-oxygenated campesterol and sitosterol in human serum. / Husche, Constanze; Weingaertner, Oliver; Pettersson, Hanna et al.
In: Chemistry and Physics of Lipids, Vol. 164, No. 6, 09.2011, p. 425-431.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Validation of an isotope dilution gas chromatography-mass spectrometry method for analysis of 7-oxygenated campesterol and sitosterol in human serum

AU - Husche, Constanze

AU - Weingaertner, Oliver

AU - Pettersson, Hanna

AU - Vanmierlo, Tim

AU - Boehm, Michael

AU - Laufs, Ulrich

AU - Lütjohann, Dieter

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N2 - High dose daily intake of plant sterols decreases the uptake of cholesterol in the intestine by competitive mechanisms and thus leads to reduced serum levels of total and LDL-cholesterol. By this, the commercialization of plant sterol enriched 'functional food' products is rapidly increasing. Subjects using these kinds of diet present a duplication of their serum plant sterol levels after long-term intake. In analogy to cholesterol, plant sterols such as campesterol and sitosterol can be oxidized to oxyphytosterols and these may counteract the primary anti-atherosclerotic action of cholesterol lowering. In order to investigate the whole spectrum of the consequences following high plant sterol intake a highly sensitive and specific isotope dilution gas chromatography-mass spectrometry method for the analysis of 7-oxygenated campesterol/sitosterol in trace amounts in human serum is presented in this paper. The validation was based on limits for detection and quantification, recovery, precision and minimization of autoxidation during work-up. Our results show an overall coefficient of variation

AB - High dose daily intake of plant sterols decreases the uptake of cholesterol in the intestine by competitive mechanisms and thus leads to reduced serum levels of total and LDL-cholesterol. By this, the commercialization of plant sterol enriched 'functional food' products is rapidly increasing. Subjects using these kinds of diet present a duplication of their serum plant sterol levels after long-term intake. In analogy to cholesterol, plant sterols such as campesterol and sitosterol can be oxidized to oxyphytosterols and these may counteract the primary anti-atherosclerotic action of cholesterol lowering. In order to investigate the whole spectrum of the consequences following high plant sterol intake a highly sensitive and specific isotope dilution gas chromatography-mass spectrometry method for the analysis of 7-oxygenated campesterol/sitosterol in trace amounts in human serum is presented in this paper. The validation was based on limits for detection and quantification, recovery, precision and minimization of autoxidation during work-up. Our results show an overall coefficient of variation

KW - Plant sterol

KW - Oxyphytosterol

KW - Low-density lipoprotein

KW - Gas chromatograpy-mass spectrometry

KW - Atherosclerosis

KW - Cardiovascular disease

U2 - 10.1016/j.chemphyslip.2011.04.009

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