Validation of an isotope dilution gas chromatography-mass spectrometry method for analysis of 7-oxygenated campesterol and sitosterol in human serum

Constanze Husche, Oliver Weingaertner, Hanna Pettersson, Tim Vanmierlo, Michael Boehm, Ulrich Laufs, Dieter Lütjohann*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

39 Citations (Web of Science)

Abstract

High dose daily intake of plant sterols decreases the uptake of cholesterol in the intestine by competitive mechanisms and thus leads to reduced serum levels of total and LDL-cholesterol. By this, the commercialization of plant sterol enriched 'functional food' products is rapidly increasing. Subjects using these kinds of diet present a duplication of their serum plant sterol levels after long-term intake. In analogy to cholesterol, plant sterols such as campesterol and sitosterol can be oxidized to oxyphytosterols and these may counteract the primary anti-atherosclerotic action of cholesterol lowering. In order to investigate the whole spectrum of the consequences following high plant sterol intake a highly sensitive and specific isotope dilution gas chromatography-mass spectrometry method for the analysis of 7-oxygenated campesterol/sitosterol in trace amounts in human serum is presented in this paper. The validation was based on limits for detection and quantification, recovery, precision and minimization of autoxidation during work-up. Our results show an overall coefficient of variation
Original languageEnglish
Pages (from-to)425-431
JournalChemistry and Physics of Lipids
Volume164
Issue number6
DOIs
Publication statusPublished - Sep 2011

Keywords

  • Plant sterol
  • Oxyphytosterol
  • Low-density lipoprotein
  • Gas chromatograpy-mass spectrometry
  • Atherosclerosis
  • Cardiovascular disease

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