TY - JOUR
T1 - Utilization of flax fibers for biomedical applications
AU - Michel, Sophie A. A. X.
AU - Vogels, Ruben R. M.
AU - Bouvy, Nicole D.
AU - Knetsch, Menno L. W.
AU - van den Akker, Nynke M. S.
AU - Gijbels, Marion J. J.
AU - van der Marel, Cees
AU - Vermeersch, Jan
AU - Molin, Daniel G. M.
AU - Koole, Leo H.
PY - 2014/4
Y1 - 2014/4
N2 - Over the past decades, a large number of animal-derived materials have been introduced for several biomedical applications. Surprisingly, the use of plant-based materials has lagged behind. To study the feasibility of plant-derived biomedical materials, we chose flax (Linum usitatissimum). Flax fibers possess excellent physical-mechanical properties, are nonbiodegradable, and there is extensive know-how on weaving/knitting of them. One area where they could be useful is as implantable mesh structures in surgery, in particular for the repair of incisional hernias of the abdominal wall. Starting with a bleached flax thread, a prototype mesh was specifically knitted for this study, and its cytocompatibility was studied in vitro and in vivo. The experimental data revealed that application of flax in surgery first requires a robust method to remove endotoxins and purify the flax fiber. Such a method was developed, and purified meshes did not cause loss of cell viability in vitro. In addition, endotoxins determined using limulus amebocyte lysate test were at acceptable levels. In vivo, the flax meshes showed only mild inflammation, comparable to commercial polypropylene meshes. This study revealed that plant-derived biomaterials can provide a new class of implantable materials that could be used as surgical meshes or for other biomedical applications.
AB - Over the past decades, a large number of animal-derived materials have been introduced for several biomedical applications. Surprisingly, the use of plant-based materials has lagged behind. To study the feasibility of plant-derived biomedical materials, we chose flax (Linum usitatissimum). Flax fibers possess excellent physical-mechanical properties, are nonbiodegradable, and there is extensive know-how on weaving/knitting of them. One area where they could be useful is as implantable mesh structures in surgery, in particular for the repair of incisional hernias of the abdominal wall. Starting with a bleached flax thread, a prototype mesh was specifically knitted for this study, and its cytocompatibility was studied in vitro and in vivo. The experimental data revealed that application of flax in surgery first requires a robust method to remove endotoxins and purify the flax fiber. Such a method was developed, and purified meshes did not cause loss of cell viability in vitro. In addition, endotoxins determined using limulus amebocyte lysate test were at acceptable levels. In vivo, the flax meshes showed only mild inflammation, comparable to commercial polypropylene meshes. This study revealed that plant-derived biomaterials can provide a new class of implantable materials that could be used as surgical meshes or for other biomedical applications.
KW - flax
KW - implantable mesh
KW - biocompatibility/soft tissue
KW - cell-material interactions
KW - fiber
U2 - 10.1002/jbm.b.33025
DO - 10.1002/jbm.b.33025
M3 - Article
C2 - 24039184
SN - 1552-4973
VL - 102
SP - 477
EP - 487
JO - Journal of Biomedical Materials Research Part B-applied Biomaterials
JF - Journal of Biomedical Materials Research Part B-applied Biomaterials
IS - 3
ER -