Use of microfluidics to assess the platelet-based control of coagulation

Magdolna Nagy, Johan W. M. Heemskerk, Frauke Swieringa*

*Corresponding author for this work

Research output: Contribution to journal(Systematic) Review article peer-review

25 Citations (Web of Science)

Abstract

This paper provides an overview of the various types of microfluidic devices that are employed to study the complex processes of platelet activation and blood coagulation in whole blood under flow conditions. We elaborate on how these devices are used to detect impaired platelet-dependent fibrin formation in blood from mice or patients with specific bleeding disorders. We provide a practical guide on how to assess formation of a platelet-fibrin thrombus under flow, using equipment that is present in most laboratories. In addition, we describe current insights on how blood flow and shear rate alter the location of platelet populations, von Willebrand factor, coagulation factors, and fibrin in a growing thrombus. Finally, we discuss possibilities and limitations for the clinical use of microfluidic devices to evaluate a hemostatic or prothrombotic tendency in patient blood samples.

Original languageEnglish
Pages (from-to)441-448
Number of pages8
JournalPlatelets
Volume28
Issue number5
DOIs
Publication statusPublished - 2017

Keywords

  • Fibrin
  • platelet
  • thrombin
  • thrombus
  • FLOW-CHAMBER SYSTEM
  • PERCUTANEOUS CORONARY INTERVENTION
  • DEPENDENT THROMBUS FORMATION
  • WILLEBRAND DISEASE PATIENTS
  • WALL SHEAR RATE
  • TISSUE FACTOR
  • PROCOAGULANT ACTIVITY
  • FIBRIN DEPOSITION
  • BLOOD-COAGULATION
  • PRESSURE-GRADIENT

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