Urinary peptidomic biomarkers of renal function in heart transplant recipients

Qi-Fang Huang; Zhen-Yu Zhang; Jan Van Keer; Sander Trenson; Esther Nkuipou-Kenfack; Wen-Yi Yang; Lutgarde Thijs; Johan Vanhaecke; Lucas N. L. Van Aelst; Johan Van Cleemput; Stefan Janssens; Peter Verhamme; Harald Mischak; Jan A. Staessen

doi:10.1093/ndt/gfy185

Urinary peptidomic biomarkers of renal function in heart transplant recipients

Qi-Fang Huang, Zhen-Yu Zhang, Jan Van Keer, Sander Trenson, Esther Nkuipou-Kenfack, Wen-Yi Yang, Lutgarde Thijs, Johan Vanhaecke, Lucas N. L. Van Aelst, Johan Van Cleemput, Stefan Janssens, Peter Verhamme, Harald Mischak, Jan A. Staessen^*

^*Corresponding author for this work

Carim - Hypertension and target organ damage

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Background. Chronic kidney disease (CKD) is common in patients after heart transplantation (HTx). We assessed whether in HTx recipients the proteomic urinary classifier CKD273 or sequenced urinary peptides revealing the parental proteins correlated with the estimated glomerular filtration rate (eGFR).

Methods. In 368 HTx patients, we measured the urinary peptidome and analysed CKD273 and 48 urinary peptides with a detectable signal in >95% of participants. After 9.1months (median), eGFR and the urinary biomarkers were reassessed.

Results. In multivariable Bonferroni-corrected analyses of the baseline data, a 1-SD increase in CKD273 was associated with a 11.4 [95% confidence interval (CI) 7.25-15.5] mL/min/1.73m(2) lower eGFR and an odds ratio of 2.63 (1.56-4.46) for having eGFR

Conclusions. With the exception of baseline eGFR, CKD273 was more closer associated with imminent renal dysfunction than 24-h proteinuria. Fragments of collagen I and mucin-1-respectively, positively and inversely associated with eGFR and change in eGFR-are single-peptide markers associated with renal dysfunction.

Original language	English
Pages (from-to)	1336-1343
Number of pages	8
Journal	Nephrology Dialysis Transplantation
Volume	34
Issue number	8
DOIs	https://doi.org/10.1093/ndt/gfy185
Publication status	Published - Aug 2019

Keywords

DIAGNOSIS
DISEASE
EXPRESSION
KIDNEY
KL-6
MANAGEMENT
MUC1
MUTATIONS
PREDICTION
PROTEOME
SERUM
collagen
glomerular filtration
heart transplantation
mucin-1
urinary proteomics

Access to Document

10.1093/ndt/gfy185Licence: CC BY-NC

Cite this

Huang, Q.-F., Zhang, Z.-Y., Van Keer, J., Trenson, S., Nkuipou-Kenfack, E., Yang, W.-Y., Thijs, L., Vanhaecke, J., Van Aelst, L. N. L., Van Cleemput, J., Janssens, S., Verhamme, P., Mischak, H., & Staessen, J. A. (2019). Urinary peptidomic biomarkers of renal function in heart transplant recipients. Nephrology Dialysis Transplantation, 34(8), 1336-1343. https://doi.org/10.1093/ndt/gfy185

@article{c17315b9ef6149b88f8a6b3c554c286b,

title = "Urinary peptidomic biomarkers of renal function in heart transplant recipients",

abstract = "Background. Chronic kidney disease (CKD) is common in patients after heart transplantation (HTx). We assessed whether in HTx recipients the proteomic urinary classifier CKD273 or sequenced urinary peptides revealing the parental proteins correlated with the estimated glomerular filtration rate (eGFR).Methods. In 368 HTx patients, we measured the urinary peptidome and analysed CKD273 and 48 urinary peptides with a detectable signal in >95% of participants. After 9.1months (median), eGFR and the urinary biomarkers were reassessed.Results. In multivariable Bonferroni-corrected analyses of the baseline data, a 1-SD increase in CKD273 was associated with a 11.4 [95% confidence interval (CI) 7.25-15.5] mL/min/1.73m(2) lower eGFR and an odds ratio of 2.63 (1.56-4.46) for having eGFRConclusions. With the exception of baseline eGFR, CKD273 was more closer associated with imminent renal dysfunction than 24-h proteinuria. Fragments of collagen I and mucin-1-respectively, positively and inversely associated with eGFR and change in eGFR-are single-peptide markers associated with renal dysfunction.",

keywords = "DIAGNOSIS, DISEASE, EXPRESSION, KIDNEY, KL-6, MANAGEMENT, MUC1, MUTATIONS, PREDICTION, PROTEOME, SERUM, collagen, glomerular filtration, heart transplantation, mucin-1, urinary proteomics",

author = "Qi-Fang Huang and Zhen-Yu Zhang and {Van Keer}, Jan and Sander Trenson and Esther Nkuipou-Kenfack and Wen-Yi Yang and Lutgarde Thijs and Johan Vanhaecke and {Van Aelst}, {Lucas N. L.} and {Van Cleemput}, Johan and Stefan Janssens and Peter Verhamme and Harald Mischak and Staessen, {Jan A.}",

note = "Funding Information: The European Union (HEALTH-F7-305507 HOMAGE), the European Research Council (Advanced Researcher Grant 2011-294713-EPLORE and Proof-of-Concept Grant 713601-uPROPHET), the European Research Area Net for Cardiovascular Diseases (JTC2017-046-PROACT) and the Fonds voor Wetenschappelijk Onderzoek Vlaanderen, Ministry of the Flemish Community, Brussels, Belgium (G.0881.13) currently support the Studies Coordinating Centre in Leuven. Publisher Copyright: {\textcopyright} 2018 The Author(s) 2018. Published by Oxford University Press on behalf of ERA-EDTA.",

year = "2019",

month = aug,

doi = "10.1093/ndt/gfy185",

language = "English",

volume = "34",

pages = "1336--1343",

journal = "Nephrology Dialysis Transplantation",

issn = "0931-0509",

publisher = "Oxford University Press",

number = "8",

}

Huang, Q-F, Zhang, Z-Y, Van Keer, J, Trenson, S, Nkuipou-Kenfack, E, Yang, W-Y, Thijs, L, Vanhaecke, J, Van Aelst, LNL, Van Cleemput, J, Janssens, S, Verhamme, P, Mischak, H & Staessen, JA 2019, 'Urinary peptidomic biomarkers of renal function in heart transplant recipients', Nephrology Dialysis Transplantation, vol. 34, no. 8, pp. 1336-1343. https://doi.org/10.1093/ndt/gfy185

TY - JOUR

T1 - Urinary peptidomic biomarkers of renal function in heart transplant recipients

AU - Huang, Qi-Fang

AU - Zhang, Zhen-Yu

AU - Van Keer, Jan

AU - Trenson, Sander

AU - Nkuipou-Kenfack, Esther

AU - Yang, Wen-Yi

AU - Thijs, Lutgarde

AU - Vanhaecke, Johan

AU - Van Aelst, Lucas N. L.

AU - Van Cleemput, Johan

AU - Janssens, Stefan

AU - Verhamme, Peter

AU - Mischak, Harald

AU - Staessen, Jan A.

N1 - Funding Information: The European Union (HEALTH-F7-305507 HOMAGE), the European Research Council (Advanced Researcher Grant 2011-294713-EPLORE and Proof-of-Concept Grant 713601-uPROPHET), the European Research Area Net for Cardiovascular Diseases (JTC2017-046-PROACT) and the Fonds voor Wetenschappelijk Onderzoek Vlaanderen, Ministry of the Flemish Community, Brussels, Belgium (G.0881.13) currently support the Studies Coordinating Centre in Leuven. Publisher Copyright: © 2018 The Author(s) 2018. Published by Oxford University Press on behalf of ERA-EDTA.

PY - 2019/8

Y1 - 2019/8

N2 - Background. Chronic kidney disease (CKD) is common in patients after heart transplantation (HTx). We assessed whether in HTx recipients the proteomic urinary classifier CKD273 or sequenced urinary peptides revealing the parental proteins correlated with the estimated glomerular filtration rate (eGFR).Methods. In 368 HTx patients, we measured the urinary peptidome and analysed CKD273 and 48 urinary peptides with a detectable signal in >95% of participants. After 9.1months (median), eGFR and the urinary biomarkers were reassessed.Results. In multivariable Bonferroni-corrected analyses of the baseline data, a 1-SD increase in CKD273 was associated with a 11.4 [95% confidence interval (CI) 7.25-15.5] mL/min/1.73m(2) lower eGFR and an odds ratio of 2.63 (1.56-4.46) for having eGFRConclusions. With the exception of baseline eGFR, CKD273 was more closer associated with imminent renal dysfunction than 24-h proteinuria. Fragments of collagen I and mucin-1-respectively, positively and inversely associated with eGFR and change in eGFR-are single-peptide markers associated with renal dysfunction.

AB - Background. Chronic kidney disease (CKD) is common in patients after heart transplantation (HTx). We assessed whether in HTx recipients the proteomic urinary classifier CKD273 or sequenced urinary peptides revealing the parental proteins correlated with the estimated glomerular filtration rate (eGFR).Methods. In 368 HTx patients, we measured the urinary peptidome and analysed CKD273 and 48 urinary peptides with a detectable signal in >95% of participants. After 9.1months (median), eGFR and the urinary biomarkers were reassessed.Results. In multivariable Bonferroni-corrected analyses of the baseline data, a 1-SD increase in CKD273 was associated with a 11.4 [95% confidence interval (CI) 7.25-15.5] mL/min/1.73m(2) lower eGFR and an odds ratio of 2.63 (1.56-4.46) for having eGFRConclusions. With the exception of baseline eGFR, CKD273 was more closer associated with imminent renal dysfunction than 24-h proteinuria. Fragments of collagen I and mucin-1-respectively, positively and inversely associated with eGFR and change in eGFR-are single-peptide markers associated with renal dysfunction.

KW - DIAGNOSIS

KW - DISEASE

KW - EXPRESSION

KW - KIDNEY

KW - KL-6

KW - MANAGEMENT

KW - MUC1

KW - MUTATIONS

KW - PREDICTION

KW - PROTEOME

KW - SERUM

KW - collagen

KW - glomerular filtration

KW - heart transplantation

KW - mucin-1

KW - urinary proteomics

U2 - 10.1093/ndt/gfy185

DO - 10.1093/ndt/gfy185

M3 - Article

C2 - 29982668

SN - 0931-0509

VL - 34

SP - 1336

EP - 1343

JO - Nephrology Dialysis Transplantation

JF - Nephrology Dialysis Transplantation

IS - 8

ER -

Urinary peptidomic biomarkers of renal function in heart transplant recipients

Abstract

Keywords

Access to Document

Fingerprint

Cite this