Two-Photon Endoscopy: State of the Art and Perspectives

V. Kucikas*, M.P. Werner, T. Schmitz-Rode, F. Louradour, M.A.M.J. van Zandvoort

*Corresponding author for this work

Research output: Contribution to journalReview articleAcademicpeer-review

Abstract

In recent years, the demand for non-destructive deep-tissue imaging modalities has led to interest in multiphoton endoscopy. In contrast to bench top systems, multiphoton endoscopy enables subcellular resolution imaging in areas not reachable before. Several groups have recently presented their development towards the goal of producing user friendly plug and play system, which could be used in biological research and, potentially, clinical applications. We first present the technological challenges, prerequisites, and solutions in two-photon endoscopic systems. Secondly, we focus on the applications already found in literature. These applications mostly serve as a quality check of the built system, but do not answer a specific biomedical research question. Therefore, in the last part, we will describe our vision on the enormous potential applicability of adult two-photon endoscopic systems in biological and clinical research. We will thus bring forward the concept that two-photon endoscopy is a sine qua non in bringing this technique to the forefront in clinical applications.
Original languageEnglish
Number of pages15
JournalMolecular Imaging and Biology
DOIs
Publication statusE-pub ahead of print - 15 Nov 2021

Keywords

  • Multiphoton endoscopy
  • Nonlinear endoscopy
  • In vivo imaging
  • Label free
  • Kidney imaging
  • Colon imaging
  • MEMS SCANNING CATHETER
  • PHOTONIC CRYSTAL FIBER
  • HIGH-RESOLUTION
  • DISPERSION COMPENSATION
  • PULSE-COMPRESSION
  • FEMTOSECOND PULSES
  • REFLECTION GRISMS
  • ENDOMICROSCOPY
  • MICROSCOPE
  • COMPACT

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