Trends in mass spectrometry imaging for cardiovascular diseases

Stephanie T. P. Mezger, Alma M. A. Mingels, Otto Bekers, Berta Cillero-Pastor, Ron M. A. Heeren*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

18 Citations (Web of Science)

Abstract

Mass spectrometry imaging (MSI) is a widely established technology; however, in the cardiovascular research field, its use is still emerging. The technique has the advantage of analyzing multiple molecules without prior knowledge while maintaining the relation with tissue morphology. Particularly, MALDI-based approaches have been applied to obtain in-depth knowledge of cardiac (dys)function. Here, we discuss the different aspects of the MSI protocols, from sample handling to instrumentation used in cardiovascular research, and critically evaluate these methods. The trend towards structural lipid analysis, identification, and top-down protein MSI shows the potential for implementation in (pre)clinical research and complementing the diagnostic tests. Moreover, new insights into disease progression are expected and thereby contribute to the understanding of underlying mechanisms related to cardiovascular diseases.

Original languageEnglish
Pages (from-to)3709-3720
Number of pages12
JournalAnalytical and Bioanalytical Chemistry
Volume411
Issue number17
DOIs
Publication statusPublished - Jul 2019

Keywords

  • Mass spectrometry imaging
  • Cardiovascular diseases
  • MALDI
  • SIMS
  • Lipids
  • Proteins
  • MOUSE HEART
  • ATHEROSCLEROTIC PLAQUE
  • RAT-HEART
  • TISSUE
  • LIPIDS
  • MS
  • METABOLITES
  • PROTEOMICS
  • PROTEINS
  • REVEALS

Cite this