Translational control is a major contributor to hypoxia induced gene expression

Twan van den Beucken, Michael G. Magagnin, Barry Jutten, Renaud Seigneuric, Philippe Lambin, Marianne Koritzinsky, Bradly G. Wouters*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

Background and purpose: Hypoxia is a common feature of solid tumors that is associated with an aggressive phenotype, resistance to therapy and poor prognosis. Major contributors to these adverse effects are the transcriptional program activated by the HIF family of transcription factors as well as the translational response mediated by PERK-dependent phosphorylation of elF2 alpha and inhibition of mTORC1 activity. In this study we determined the relative contribution of both transcriptional and translational responses to changes in hypoxia induced gene expression. Material and methods: Total and efficiently translated (polysomal) mRNA was isolated from DU145 prostate carcinoma cells that were exposed for up to 24 h of hypoxia (
Original languageEnglish
Pages (from-to)379-384
JournalRadiotherapy and Oncology
Volume99
Issue number3
DOIs
Publication statusPublished - Jun 2011

Keywords

  • Hypoxia
  • Translation
  • Gene expression
  • HIF
  • UPR

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