Translational control is a major contributor to hypoxia induced gene expression

Twan van den Beucken; Michael G. Magagnin; Barry Jutten; Renaud Seigneuric; Philippe Lambin; Marianne Koritzinsky; Bradly G. Wouters

doi:10.1016/j.radonc.2011.05.058

Translational control is a major contributor to hypoxia induced gene expression

Twan van den Beucken, Michael G. Magagnin, Barry Jutten, Renaud Seigneuric, Philippe Lambin, Marianne Koritzinsky, Bradly G. Wouters^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Background and purpose: Hypoxia is a common feature of solid tumors that is associated with an aggressive phenotype, resistance to therapy and poor prognosis. Major contributors to these adverse effects are the transcriptional program activated by the HIF family of transcription factors as well as the translational response mediated by PERK-dependent phosphorylation of elF2 alpha and inhibition of mTORC1 activity. In this study we determined the relative contribution of both transcriptional and translational responses to changes in hypoxia induced gene expression. Material and methods: Total and efficiently translated (polysomal) mRNA was isolated from DU145 prostate carcinoma cells that were exposed for up to 24 h of hypoxia (

Original language	English
Pages (from-to)	379-384
Journal	Radiotherapy and Oncology
Volume	99
Issue number	3
DOIs	https://doi.org/10.1016/j.radonc.2011.05.058
Publication status	Published - Jun 2011

Keywords

Hypoxia
Translation
Gene expression
HIF
UPR

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10.1016/j.radonc.2011.05.058Licence: Free access - publisher

Full TextFinal published version, 646 KBLicence: Taverne

Cite this

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title = "Translational control is a major contributor to hypoxia induced gene expression",

abstract = "Background and purpose: Hypoxia is a common feature of solid tumors that is associated with an aggressive phenotype, resistance to therapy and poor prognosis. Major contributors to these adverse effects are the transcriptional program activated by the HIF family of transcription factors as well as the translational response mediated by PERK-dependent phosphorylation of elF2 alpha and inhibition of mTORC1 activity. In this study we determined the relative contribution of both transcriptional and translational responses to changes in hypoxia induced gene expression. Material and methods: Total and efficiently translated (polysomal) mRNA was isolated from DU145 prostate carcinoma cells that were exposed for up to 24 h of hypoxia (",

keywords = "Hypoxia, Translation, Gene expression, HIF, UPR",

author = "{van den Beucken}, Twan and Magagnin, {Michael G.} and Barry Jutten and Renaud Seigneuric and Philippe Lambin and Marianne Koritzinsky and Wouters, {Bradly G.}",

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T1 - Translational control is a major contributor to hypoxia induced gene expression

AU - van den Beucken, Twan

AU - Magagnin, Michael G.

AU - Jutten, Barry

AU - Seigneuric, Renaud

AU - Lambin, Philippe

AU - Koritzinsky, Marianne

AU - Wouters, Bradly G.

PY - 2011/6

Y1 - 2011/6

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AB - Background and purpose: Hypoxia is a common feature of solid tumors that is associated with an aggressive phenotype, resistance to therapy and poor prognosis. Major contributors to these adverse effects are the transcriptional program activated by the HIF family of transcription factors as well as the translational response mediated by PERK-dependent phosphorylation of elF2 alpha and inhibition of mTORC1 activity. In this study we determined the relative contribution of both transcriptional and translational responses to changes in hypoxia induced gene expression. Material and methods: Total and efficiently translated (polysomal) mRNA was isolated from DU145 prostate carcinoma cells that were exposed for up to 24 h of hypoxia (

KW - Hypoxia

KW - Translation

KW - Gene expression

KW - HIF

KW - UPR

U2 - 10.1016/j.radonc.2011.05.058

DO - 10.1016/j.radonc.2011.05.058

M3 - Article

C2 - 21719133

SN - 0167-8140

VL - 99

SP - 379

EP - 384

JO - Radiotherapy and Oncology

JF - Radiotherapy and Oncology

IS - 3

ER -