Transcriptomics analysis of retinoic acid embryotoxicity in rat postimplantation whole embryo culture.

M. Luijten, V.A. van Beelen, A. Verhoef, M.F. Renkens, M.H. van Herwijnen, A. Westerman, F.J. van Schooten, J.L. Pennings, A.H. Piersma

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    Abstract

    Rodent postimplantation whole embryo culture (WEC) is a classical alternative test to study developmental toxicants. Here, we have successfully applied transcriptomics to monitor early responses in WEC after exposure to the embryotoxicant retinoic acid (RA). We demonstrated that RA exposures ranging from 2 to 24hours affect RA-responsive genes in individual embryos. Furthermore, 2-, 3- or 4-somite embryos gave similar responses, allowing combining embryos of these embryonic stages within the same analysis. Microarray analysis of embryonic gene expression after RA exposure revealed the regulation of many genes known to be RA responsive. Finally, use of a culture medium based on bovine serum instead of rat serum yielded similar gene expression responses after RA exposure. These findings support the robustness of the identified gene expression patterns and show the feasibility of detecting early gene expression changes in WEC after embryotoxic exposures. This approach may result in a more sensitive readout for detecting embryotoxicity in WEC.
    Original languageEnglish
    Pages (from-to)333-340
    Number of pages8
    JournalReproductive Toxicology
    Volume30
    Issue number2
    DOIs
    Publication statusPublished - Sep 2010

    Keywords

    • Embryotoxicity
    • Retinoic acid
    • Microarray analysis
    • Rat serum
    • Bovine serum
    • Whole embryo culture
    • GENE-EXPRESSION
    • IN-VITRO
    • HINDBRAIN
    • MOUSE
    • SEGMENTATION
    • VALIDATION
    • KROX-20
    • INVITRO
    • TESTS
    • CDX1

    Cite this

    Luijten, M., van Beelen, V. A., Verhoef, A., Renkens, M. F., van Herwijnen, M. H., Westerman, A., van Schooten, F. J., Pennings, J. L., & Piersma, A. H. (2010). Transcriptomics analysis of retinoic acid embryotoxicity in rat postimplantation whole embryo culture. Reproductive Toxicology, 30(2), 333-340. https://doi.org/10.1016/j.reprotox.2010.05.013