Long-chain saturated fatty acids such as palmitic acid induce insulin resistance and NF-kappaB activation in skeletal muscle cells. Here we investigated the effects of long-chain fatty acid (FA) saturation and configuration on NF-kappaB activity and insulin sensitivity in cultured skeletal muscle cells. Of all tested unsaturated FAs, only elaidic acid (3-fold), cis9,trans11-CLA (3-fold) and trans10,cis12-CLA (13-fold) increased NF-kappaB transactivation in myotubes. This was not accompanied by decreased insulin sensitivity (measured as insulin-induced glucose uptake and GLUT4 translocation). We therefore conclude that FA-induced NF-kappaB activation is not sufficient for the induction of insulin resistance in skeletal muscle cells.
Hommelberg, P. P., Langen, R. C. J., Schols, A. M. W. J., van Essen, A. L., Snepvangers, F. J., Mensink, R. P., & Plat, J. (2010). Trans Fatty Acid-Induced NF-kappaB Activation Does Not Induce Insulin Resistance in Cultured Murine Skeletal Muscle Cells. Lipids, 45(3), 285-290. https://doi.org/10.1007/s11745-010-3388-1