Toxicity testing of pesticides in zebrafish-a systematic review on chemicals and associated toxicological endpoints

Iris Flavia Sousa Goncalves, Terezinha Maria Souza*, Leonardo Rogerio Vieira, Filipi Calbaizer Marchi, Adailton Pascoal Nascimento, Davi Felipe Farias*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

19 Citations (Web of Science)

Abstract

The use of zebrafish (Danio rerio) has arisen as a promising biological platform for toxicity testing of pesticides such as herbicides, insecticides, and fungicides. Therefore, it is relevant to assess the use of zebrafish in models of exposure to investigate the diversity of pesticide-associated toxicity endpoints which have been reported. Thus, this review aimed to assess the recent literature on the use of zebrafish in pesticide toxicity studies to capture data on the types of pesticide used, classes of pesticides, and zebrafish life stages associated with toxicity endpoints and phenotypic observations. A total of 352 articles published between September 2012 and May 2019 were curated. The results show an increased trend in the use of zebrafish for testing the toxicity of pesticides, with a great diversity of pesticides (203) and chemical classes (58) with different applications (41) being used. Furthermore, experimental outcomes could be clustered in 13 toxicity endpoints, mainly developmental toxicity, oxidative stress, and neurotoxicity. Organophosphorus, pyrethroid, azole, and triazine were the most studied classes of pesticides and associated with various toxicity endpoints. Studies frequently opted for early life stages (embryos and larvae). Although there is an evident lack of standardization of nomenclatures and phenotypic alterations, the information gathered here highlights associations between (classes of) pesticides and endpoints, which can be used to relate mechanisms of action specific to certain classes of chemicals.

Original languageEnglish
Pages (from-to)10185-10204
Number of pages20
JournalEnvironmental Science and Pollution Research
Volume27
Issue number10
DOIs
Publication statusPublished - Apr 2020

Keywords

  • Danio rerio
  • Fungicide
  • Herbicide
  • Insecticide
  • Organophosphorus
  • Pesticide toxicity
  • Toxicity endpoints
  • Zebrafish embryos
  • PERSISTENT ORGANIC POLLUTANTS
  • EARLY-LIFE STAGES
  • DANIO-RERIO
  • OXIDATIVE STRESS
  • DEVELOPMENTAL TOXICITY
  • ENDOCRINE DISRUPTION
  • EMBRYONIC-DEVELOPMENT
  • LOCOMOTOR-ACTIVITY
  • IN-VIVO
  • CARDIOVASCULAR TOXICITY

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