TY - JOUR
T1 - Toxicity testing of pesticides in zebrafish-a systematic review on chemicals and associated toxicological endpoints
AU - Sousa Goncalves, Iris Flavia
AU - Souza, Terezinha Maria
AU - Vieira, Leonardo Rogerio
AU - Marchi, Filipi Calbaizer
AU - Nascimento, Adailton Pascoal
AU - Farias, Davi Felipe
N1 - Funding Information:
We thank to Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Brazil, for supporting this research with grants and scholarships, and to Ms. Marta Silva Muniz for contributing with revision of the English manuscript.
Funding Information:
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Brazil, grant number 461182-9. Acknowledgments
Funding Information:
We thank to Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico (CNPq), Brazil, for supporting this research with grants and scholarships, and to Ms. Marta Silva Muniz for contributing with revision of the English manuscript.
Publisher Copyright:
© 2020, Springer-Verlag GmbH Germany, part of Springer Nature.
PY - 2020/4
Y1 - 2020/4
N2 - The use of zebrafish (Danio rerio) has arisen as a promising biological platform for toxicity testing of pesticides such as herbicides, insecticides, and fungicides. Therefore, it is relevant to assess the use of zebrafish in models of exposure to investigate the diversity of pesticide-associated toxicity endpoints which have been reported. Thus, this review aimed to assess the recent literature on the use of zebrafish in pesticide toxicity studies to capture data on the types of pesticide used, classes of pesticides, and zebrafish life stages associated with toxicity endpoints and phenotypic observations. A total of 352 articles published between September 2012 and May 2019 were curated. The results show an increased trend in the use of zebrafish for testing the toxicity of pesticides, with a great diversity of pesticides (203) and chemical classes (58) with different applications (41) being used. Furthermore, experimental outcomes could be clustered in 13 toxicity endpoints, mainly developmental toxicity, oxidative stress, and neurotoxicity. Organophosphorus, pyrethroid, azole, and triazine were the most studied classes of pesticides and associated with various toxicity endpoints. Studies frequently opted for early life stages (embryos and larvae). Although there is an evident lack of standardization of nomenclatures and phenotypic alterations, the information gathered here highlights associations between (classes of) pesticides and endpoints, which can be used to relate mechanisms of action specific to certain classes of chemicals.
AB - The use of zebrafish (Danio rerio) has arisen as a promising biological platform for toxicity testing of pesticides such as herbicides, insecticides, and fungicides. Therefore, it is relevant to assess the use of zebrafish in models of exposure to investigate the diversity of pesticide-associated toxicity endpoints which have been reported. Thus, this review aimed to assess the recent literature on the use of zebrafish in pesticide toxicity studies to capture data on the types of pesticide used, classes of pesticides, and zebrafish life stages associated with toxicity endpoints and phenotypic observations. A total of 352 articles published between September 2012 and May 2019 were curated. The results show an increased trend in the use of zebrafish for testing the toxicity of pesticides, with a great diversity of pesticides (203) and chemical classes (58) with different applications (41) being used. Furthermore, experimental outcomes could be clustered in 13 toxicity endpoints, mainly developmental toxicity, oxidative stress, and neurotoxicity. Organophosphorus, pyrethroid, azole, and triazine were the most studied classes of pesticides and associated with various toxicity endpoints. Studies frequently opted for early life stages (embryos and larvae). Although there is an evident lack of standardization of nomenclatures and phenotypic alterations, the information gathered here highlights associations between (classes of) pesticides and endpoints, which can be used to relate mechanisms of action specific to certain classes of chemicals.
KW - Danio rerio
KW - Fungicide
KW - Herbicide
KW - Insecticide
KW - Organophosphorus
KW - Pesticide toxicity
KW - Toxicity endpoints
KW - Zebrafish embryos
KW - PERSISTENT ORGANIC POLLUTANTS
KW - EARLY-LIFE STAGES
KW - DANIO-RERIO
KW - OXIDATIVE STRESS
KW - DEVELOPMENTAL TOXICITY
KW - ENDOCRINE DISRUPTION
KW - EMBRYONIC-DEVELOPMENT
KW - LOCOMOTOR-ACTIVITY
KW - IN-VIVO
KW - CARDIOVASCULAR TOXICITY
U2 - 10.1007/s11356-020-07902-5
DO - 10.1007/s11356-020-07902-5
M3 - (Systematic) Review article
C2 - 32062774
SN - 0944-1344
VL - 27
SP - 10185
EP - 10204
JO - Environmental Science and Pollution Research
JF - Environmental Science and Pollution Research
IS - 10
ER -