Topical Imiquimod Treatment of High-grade Cervical Intraepithelial Neoplasia (TOPIC-3): A Nonrandomized Multicenter Study

Natasja Hendriks, Margot M Koeneman, Anna J M van de Sande, Charlotte G J Penders, Jurgen M J Piek, Loes F S Kooreman, Sander M J van Kuijk, Linde Hoosemans, Simone J S Sep, Peggy J de Vos Van Steenwijk, Heleen J van Beekhuizen, Brigitte F M Slangen, Hans W Nijman, Roy F P M Kruitwagen, Arnold-Jan Kruse*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review


Topical imiquimod could be an alternative, noninvasive, treatment modality for high-grade cervical intraepithelial neoplasia (CIN). However, evidence is limited, and there are no studies that compared treatment effectiveness and side effects of topical imiquimod cream to standard large loop excision of the transformation zone (LLETZ) treatment. A multi-center, nonrandomized controlled trial was performed among women with a histologic diagnosis of CIN 2/3. Women were treated with either vaginal imiquimod (6.25 mg 3 times weekly for 8 to 16 wk) or LLETZ according to their own preference. Successful treatment was defined as the absence of high-grade dysplasia at the first follow-up interval after treatment (at 20 wk for the imiquimod group and at 26 wk for the LLETZ group). Secondary outcome measures were high-risk human papillomavirus (hrHPV) clearance, side effects, and predictive factors for successful imiquimod treatment. Imiquimod treatment was successful in 60% of women who completed imiquimod treatment and 95% of women treated with LLETZ. hrHPV clearance occurred in 69% and 67% in the imiquimod group and LLETZ group, respectively. This study provides further evidence on topical imiquimod cream as a feasible and safe treatment modality for high-grade CIN. Although the effectiveness is considerably lower than LLETZ treatment, imiquimod treatment could prevent initial surgical treatment in over 40% of women and should be offered to a selected population of women who wish to avoid (repeated) surgical treatment of high-grade CIN.

Original languageEnglish
Pages (from-to)180-186
Number of pages7
JournalJournal of Immunotherapy
Issue number3
Publication statusPublished - 1 Apr 2022


  • CIN
  • HPV
  • RISK
  • imiquimod
  • immunotherapy


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