Tools for near-atomic resolution in single-particle cryogenic electron microscopy

P.V. Afanayev

Research output: ThesisDoctoral ThesisInternal

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Abstract

Structures of biological complexes are crucial for triggering the discovery of new drugs and vaccines. Cryogenic electron microscopy (cryo-EM) is an emerging technique, that allows obtaining structures at near-atomic resolution. In this thesis a number of novel tools were developed for optimal and reliable analysis of large cryo-EM image datasets. The effectiveness of these methods was demonstrated by solving the structure of the giant worm hemoglobin complex at near-atomic resolution. The results will undoubtedly facilitate cryo-EM data processing and contribute to the development of new drugs and vaccines.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • Maastricht University
Supervisors/Advisors
  • Peters, P.J., Supervisor, External person
  • van Heel, M., Supervisor, External person
Award date21 Apr 2016
Publication statusPublished - 2016

Keywords

  • biological complexes
  • cryo-EM
  • new drugs and vaccines

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