To pool or not to pool? Impact of the use of individual and pooled fecal samples for in vitro fermentation studies

M. Aguirre, J. Ramiro-Garcia, M.E. Koenen, K. Venema

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

This study investigated the stability and the activity of the microbiota from a single and a pool of donors in the TNO in vitro model of the colon (TIM-2 system). Our findings demonstrate the suitability of the preparation of a pool of fecal sample to be used for fermentation experiments.
Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalJournal of Microbiological Methods
Volume107
DOIs
Publication statusPublished - Dec 2014

Keywords

  • In vitro model
  • Fecal inoculum preparation
  • Pooling
  • Gut microbiota
  • Fermentation
  • HUMAN LARGE-INTESTINE
  • GUT MICROBIOTA
  • MODEL
  • BACTERIA
  • OLIGOSACCHARIDES
  • METABOLISM
  • PRODUCTS
  • PATTERNS
  • STARCH
  • CORE

Cite this