Timing of physical activity in relation to liver fat content and insulin resistance

J.H.P.M. van der Velde*, S.C. Boone, E. Winters-van Eekelen, M.K.C. Hesselink, V.B. Schrauwen-Hinderling, P. Schrauwen, H.J. Lamb, F.R. Rosendaal, R. de Mutsert

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review


Aims/hypothesis: We hypothesised that the insulin-sensitising effect of physical activity depends on the timing of the activity. Here, we examined cross-sectional associations of breaks in sedentary time and timing of physical activity with liver fat content and insulin resistance in a Dutch cohort. Methods: In 775 participants of the Netherlands Epidemiology of Obesity (NEO) study, we assessed sedentary time, breaks in sedentary time and different intensities of physical activity using activity sensors, and liver fat content by magnetic resonance spectroscopy (n=256). Participants were categorised as being most active in the morning (06:00–12:00 hours), afternoon (12:00–18:00 hours) or evening (18:00–00:00 hours) or as engaging in moderate-to-vigorous-physical activity (MVPA) evenly distributed throughout the day. Most active in a certain time block was defined as spending the majority (%) of total daily MVPA in that block. We examined associations between sedentary time, breaks and timing of MVPA with liver fat content and HOMA-IR using linear regression analyses, adjusted for demographic and lifestyle factors including total body fat. Associations of timing of MVPA were additionally adjusted for total MVPA. Results: The participants (42% men) had a mean (SD) age of 56 (4) years and a mean (SD) BMI of 26.2 (4.1) kg/m 2. Total sedentary time was not associated with liver fat content or insulin resistance, whereas the amount of breaks in sedentary time was associated with higher liver fat content. Total MVPA (−5%/h [95% CI −10%/h, 0%/h]) and timing of MVPA were associated with reduced insulin resistance but not with liver fat content. Compared with participants who had an even distribution of MVPA throughout the day, insulin resistance was similar (−3% [95% CI −25%, 16%]) in those most active in morning, whereas it was reduced in participants who were most active in the afternoon (−18% [95% CI −33%, −2%]) or evening (−25% [95% CI −49%, −4%]). Conclusions/interpretation: The number of daily breaks in sedentary time was not associated with lower liver fat content or reduced insulin resistance. Moderate-to-vigorous activity in the afternoon or evening was associated with a reduction of up to 25% in insulin resistance. Further studies should assess whether timing of physical activity is also important for the occurrence of type 2 diabetes. Graphical abstract: [Figure not available: see fulltext.].

Original languageEnglish
Pages (from-to)461-471
Number of pages11
Issue number3
Early online date1 Nov 2022
Publication statusPublished - Mar 2023


  • Epidemiology
  • insulin resistance
  • Liver fat
  • Physical activity
  • Sedentary behaviour
  • Sedentary breaks
  • Timing


Dive into the research topics of 'Timing of physical activity in relation to liver fat content and insulin resistance'. Together they form a unique fingerprint.

Cite this