Three-Dimensional Printing and Angiogenesis: Tailored Agarose-Type I Collagen Blends Comprise Three-Dimensional Printability and Angiogenesis Potential for Tissue-Engineered Substitutes

Franziska Kreimendahl, Marius Köpf, Anja Lena Thiebes, Daniela F Duarte Campos, Andreas Blaeser, Thomas Schmitz-Rode, Christian Apel, Stefan Jockenhoevel, Horst Fischer

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Three-dimensional (3D) bioprinting is a promising technology for manufacturing cell-laden tissue-engineered constructs. Larger tissue substitutes, however, require a vascularized network to ensure nutrition supply. Therefore, tailored bioinks combining 3D printability and cell-induced vascularization are needed. We hypothesize that tailored hydrogel blends made of agarose-type I collagen and agarose-fibrinogen are 3D printable and will allow the formation of capillary-like structures by human umbilical vein endothelial cells and human dermal fibroblasts. Samples were casted, incubated for 14 days, and analyzed by immunohistology and two-photon laser scanning microscopy. The 3D printability of the hydrogel blends was examined using a drop-on-demand printing system. The rheological behavior was also investigated. Substantial capillary network formation was observed in agarose-type I collagen hydrogel blends with concentrations of 0.2% or 0.5% collagen and 0.5% agarose. Furthermore, storage moduli of agarose-collagen blends were significantly increased compared to those of the corresponding single components (448 Pa for 0.5% agarose, 148 Pa for 0.5% collagen, and 1551 Pa for 0.5% agarose-0.5% collagen). Neither the addition of collagen nor fibrinogen significantly impaired the printing resolution. In conclusion, we present a tailored hydrogel blend that can be printed in 3D and in parallel exhibits cell-induced vascularization capability.

Original languageEnglish
Pages (from-to)604-615
Number of pages12
JournalTissue Engineering. Part C. Methods
Volume23
Issue number10
Early online date21 Aug 2017
DOIs
Publication statusPublished - Oct 2017

Keywords

  • angiogenesis
  • 3D printing
  • agarose
  • fibrinogen
  • type I collagen
  • tissue engineering
  • CAPILLARY MORPHOGENESIS
  • HYDROGELS
  • REGENERATION
  • SCAFFOLD
  • CELLS
  • VASCULARIZATION
  • BIOFABRICATION
  • INTEGRITY
  • DENSITY
  • HELIX

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