Thiol-Ene Alginate Hydrogels as Versatile Bioinks for Bioprinting

Huey Wen Ooi, Carlos Mota, A. Tessa ten Cate, Andrea Calore, Lorenzo Moroni*, Matthew B. Baker*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

92 Citations (Web of Science)

Abstract

Bioprinting is a powerful technique that allows precise and controlled 3D deposition of biomaterials in a predesigned, customizable, and reproducible manner. Cell-laden hydrogel ("bioink") bioprinting is especially advantageous for tissue engineering applications as multiple cells and biomaterial compositions can be selectively dispensed to create spatially well-defined architectures. Despite this promise, few hydrogel systems are easily available and suitable as bioinks, with even fewer systems allowing for molecular design of mechanical and biological properties. In this study, we report the development of a norbornene functionalized alginate system as a cell-laden bioink for extrusion-based bioprinting, with a rapid UV-induced thiol ene cross-linking mechanism that avoids acrylate kinetic chain formation. The mechanical and swelling properties of the hydrogels are tunable by varying the concentration, length, and structure of dithiol PEG cross-linkers and can be further modified by postprinting secondary cross-linking with divalent ions such as calcium. The low concentrations of alginate needed (

Original languageEnglish
Pages (from-to)3390-3400
Number of pages11
JournalBiomacromolecules
Volume19
Issue number8
DOIs
Publication statusPublished - Aug 2018

Keywords

  • MATRIX ELASTICITY
  • CELL VIABILITY
  • TISSUE
  • CHEMISTRY
  • BIOFABRICATION
  • BIOMATERIALS
  • FUTURE
  • CONSTRUCTS
  • STRATEGIES
  • MORPHOLOGY

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