Therapeutic Potential of Dental Pulp Stem Cells and Leukocyte- and Platelet-Rich Fibrin for Osteoarthritis

Melissa Lo Monaco*, Pascal Gervois, Joel Beaumont, Peter Clegg, Annelies Bronckaers, Jean-Michel Vandeweerd, Ivo Lambrichts

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

11 Citations (Web of Science)

Abstract

Osteoarthritis (OA) is a degenerative and inflammatory joint disorder with cartilage loss. Dental pulp stem cells (DPSCs) can undergo chondrogenic differentiation and secrete growth factors associated with tissue repair and immunomodulation. Leukocyte- and platelet-rich fibrin (L-PRF) emerges in regenerative medicine because of its growth factor content and fibrin matrix. This study evaluates the therapeutic application of DPSCs and L-PRF in OA via immunomodulation and cartilage regeneration. Chondrogenic differentiation of DPSCs, with or without L-PRF exudate (ex) and conditioned medium (CM), and of bone marrow-mesenchymal stem cells was compared. These cells showed differential chondrogenesis. L-PRF was unable to increase cartilage-associated components. Immature murine articular chondrocytes (iMACs) were cultured with L-PRF ex, L-PRF CM, or DPSC CM. L-PRF CM had pro-survival and proliferative effects on unstimulated and cytokine-stimulated iMACs. L-PRF CM stimulated the release of IL-6 and PGE2, and increased MMP-13, TIMP-1 and IL-6 mRNA levels in cytokine-stimulated iMACs. DPSC CM increased the survival and proliferation of unstimulated iMACs. In cytokine-stimulated iMACs, DPSC CM increased TIMP-1 gene expression, whereas it inhibited nitrite release in 3D culture. We showed promising effects of DPSCs in an in vitro OA model, as they undergo chondrogenesis in vitro, stimulate the survival of chondrocytes and have immunomodulatory effects.

Original languageEnglish
Article number980
Number of pages23
JournalCells
Volume9
Issue number4
DOIs
Publication statusPublished - Apr 2020

Keywords

  • dental pulp stem cells
  • leukocyte- and platelet-rich fibrin
  • osteoarthritis
  • cartilage regeneration
  • immunomodulation
  • ENDOTHELIAL GROWTH-FACTOR
  • AUTOLOGOUS CHONDROCYTE IMPLANTATION
  • NECROSIS-FACTOR-ALPHA
  • CHONDROGENIC DIFFERENTIATION
  • BONE-MARROW
  • ARTICULAR CHONDROCYTES
  • GENE-EXPRESSION
  • PLASMA
  • PROLIFERATION
  • KNEE

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