TY - JOUR
T1 - Therapeutic Potential of Dental Pulp Stem Cells and Leukocyte- and Platelet-Rich Fibrin for Osteoarthritis
AU - Lo Monaco, Melissa
AU - Gervois, Pascal
AU - Beaumont, Joel
AU - Clegg, Peter
AU - Bronckaers, Annelies
AU - Vandeweerd, Jean-Michel
AU - Lambrichts, Ivo
N1 - Funding Information:
Acknowledgements We thank Dr. Eberhard Schleyer for his advice about analysing pharmacokinetic parameters using the Topfit computer model. This work was supported in part by a grant from the Deutsche Forschungsgemeinschaft (SFB 330).
PY - 2020/4
Y1 - 2020/4
N2 - Osteoarthritis (OA) is a degenerative and inflammatory joint disorder with cartilage loss. Dental pulp stem cells (DPSCs) can undergo chondrogenic differentiation and secrete growth factors associated with tissue repair and immunomodulation. Leukocyte- and platelet-rich fibrin (L-PRF) emerges in regenerative medicine because of its growth factor content and fibrin matrix. This study evaluates the therapeutic application of DPSCs and L-PRF in OA via immunomodulation and cartilage regeneration. Chondrogenic differentiation of DPSCs, with or without L-PRF exudate (ex) and conditioned medium (CM), and of bone marrow-mesenchymal stem cells was compared. These cells showed differential chondrogenesis. L-PRF was unable to increase cartilage-associated components. Immature murine articular chondrocytes (iMACs) were cultured with L-PRF ex, L-PRF CM, or DPSC CM. L-PRF CM had pro-survival and proliferative effects on unstimulated and cytokine-stimulated iMACs. L-PRF CM stimulated the release of IL-6 and PGE2, and increased MMP-13, TIMP-1 and IL-6 mRNA levels in cytokine-stimulated iMACs. DPSC CM increased the survival and proliferation of unstimulated iMACs. In cytokine-stimulated iMACs, DPSC CM increased TIMP-1 gene expression, whereas it inhibited nitrite release in 3D culture. We showed promising effects of DPSCs in an in vitro OA model, as they undergo chondrogenesis in vitro, stimulate the survival of chondrocytes and have immunomodulatory effects.
AB - Osteoarthritis (OA) is a degenerative and inflammatory joint disorder with cartilage loss. Dental pulp stem cells (DPSCs) can undergo chondrogenic differentiation and secrete growth factors associated with tissue repair and immunomodulation. Leukocyte- and platelet-rich fibrin (L-PRF) emerges in regenerative medicine because of its growth factor content and fibrin matrix. This study evaluates the therapeutic application of DPSCs and L-PRF in OA via immunomodulation and cartilage regeneration. Chondrogenic differentiation of DPSCs, with or without L-PRF exudate (ex) and conditioned medium (CM), and of bone marrow-mesenchymal stem cells was compared. These cells showed differential chondrogenesis. L-PRF was unable to increase cartilage-associated components. Immature murine articular chondrocytes (iMACs) were cultured with L-PRF ex, L-PRF CM, or DPSC CM. L-PRF CM had pro-survival and proliferative effects on unstimulated and cytokine-stimulated iMACs. L-PRF CM stimulated the release of IL-6 and PGE2, and increased MMP-13, TIMP-1 and IL-6 mRNA levels in cytokine-stimulated iMACs. DPSC CM increased the survival and proliferation of unstimulated iMACs. In cytokine-stimulated iMACs, DPSC CM increased TIMP-1 gene expression, whereas it inhibited nitrite release in 3D culture. We showed promising effects of DPSCs in an in vitro OA model, as they undergo chondrogenesis in vitro, stimulate the survival of chondrocytes and have immunomodulatory effects.
KW - dental pulp stem cells
KW - leukocyte- and platelet-rich fibrin
KW - osteoarthritis
KW - cartilage regeneration
KW - immunomodulation
KW - ENDOTHELIAL GROWTH-FACTOR
KW - AUTOLOGOUS CHONDROCYTE IMPLANTATION
KW - NECROSIS-FACTOR-ALPHA
KW - CHONDROGENIC DIFFERENTIATION
KW - BONE-MARROW
KW - ARTICULAR CHONDROCYTES
KW - GENE-EXPRESSION
KW - PLASMA
KW - PROLIFERATION
KW - KNEE
U2 - 10.3390/cells9040980
DO - 10.3390/cells9040980
M3 - Article
C2 - 32326610
SN - 2073-4409
VL - 9
JO - Cells
JF - Cells
IS - 4
M1 - 980
ER -