Therapeutic drug monitoring of tacrolimus with the dried blood spot method

K. Hoogtanders; J. van der Heijden; M.H. Christiaans; P. Edelbroek; J.P. van Hooff; L.M.L. Stolk

doi:10.1016/j.jpba.2006.11.023

Therapeutic drug monitoring of tacrolimus with the dried blood spot method

K. Hoogtanders, J. van der Heijden, M.H. Christiaans, P. Edelbroek, J.P. van Hooff, L.M.L. Stolk^*

^*Corresponding author for this work

NUTRIM School of Nutrition and Translational Research in Metabolism

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

In a preliminary investigation an assay for tacrolimus based on fingerprick sampling and consecutive application as a blood spot on sampling paper has been developed. The dried blood spot was analysed by HPLC-tandem mass spectrometry. The validated range was 1-30 microg/l. Intra- and inter-assay variability for precision and accuracy was <7.5% and 15%, respectively. Tacrolimus concentrations of 24 stable out patients were compared after both blood spot sampling and conventional venous sampling. Method agreement was investigated with the methods of Passing and Bablok and Bland Altman and proved suitable for clinical use. The dried blood spot method for tacrolimus seems promising for patient monitoring.

Original language	English
Pages (from-to)	658-664
Journal	Journal of Pharmaceutical and Biomedical Analysis
Volume	44
Issue number	3
DOIs	https://doi.org/10.1016/j.jpba.2006.11.023
Publication status	Published - 1 Jan 2007

Access to Document

10.1016/j.jpba.2006.11.023

Cite this

@article{90b95ed98d764101b747c1595f3f64ca,

title = "Therapeutic drug monitoring of tacrolimus with the dried blood spot method",

abstract = "In a preliminary investigation an assay for tacrolimus based on fingerprick sampling and consecutive application as a blood spot on sampling paper has been developed. The dried blood spot was analysed by HPLC-tandem mass spectrometry. The validated range was 1-30 microg/l. Intra- and inter-assay variability for precision and accuracy was <7.5% and 15%, respectively. Tacrolimus concentrations of 24 stable out patients were compared after both blood spot sampling and conventional venous sampling. Method agreement was investigated with the methods of Passing and Bablok and Bland Altman and proved suitable for clinical use. The dried blood spot method for tacrolimus seems promising for patient monitoring.",

author = "K. Hoogtanders and {van der Heijden}, J. and M.H. Christiaans and P. Edelbroek and {van Hooff}, J.P. and L.M.L. Stolk",

year = "2007",

month = jan,

day = "1",

doi = "10.1016/j.jpba.2006.11.023",

language = "English",

volume = "44",

pages = "658--664",

journal = "Journal of Pharmaceutical and Biomedical Analysis",

issn = "0731-7085",

publisher = "Elsevier",

number = "3",

}

TY - JOUR

T1 - Therapeutic drug monitoring of tacrolimus with the dried blood spot method

AU - Hoogtanders, K.

AU - van der Heijden, J.

AU - Christiaans, M.H.

AU - Edelbroek, P.

AU - van Hooff, J.P.

AU - Stolk, L.M.L.

PY - 2007/1/1

Y1 - 2007/1/1

N2 - In a preliminary investigation an assay for tacrolimus based on fingerprick sampling and consecutive application as a blood spot on sampling paper has been developed. The dried blood spot was analysed by HPLC-tandem mass spectrometry. The validated range was 1-30 microg/l. Intra- and inter-assay variability for precision and accuracy was <7.5% and 15%, respectively. Tacrolimus concentrations of 24 stable out patients were compared after both blood spot sampling and conventional venous sampling. Method agreement was investigated with the methods of Passing and Bablok and Bland Altman and proved suitable for clinical use. The dried blood spot method for tacrolimus seems promising for patient monitoring.

AB - In a preliminary investigation an assay for tacrolimus based on fingerprick sampling and consecutive application as a blood spot on sampling paper has been developed. The dried blood spot was analysed by HPLC-tandem mass spectrometry. The validated range was 1-30 microg/l. Intra- and inter-assay variability for precision and accuracy was <7.5% and 15%, respectively. Tacrolimus concentrations of 24 stable out patients were compared after both blood spot sampling and conventional venous sampling. Method agreement was investigated with the methods of Passing and Bablok and Bland Altman and proved suitable for clinical use. The dried blood spot method for tacrolimus seems promising for patient monitoring.

U2 - 10.1016/j.jpba.2006.11.023

DO - 10.1016/j.jpba.2006.11.023

M3 - Article

SN - 0731-7085

VL - 44

SP - 658

EP - 664

JO - Journal of Pharmaceutical and Biomedical Analysis

JF - Journal of Pharmaceutical and Biomedical Analysis

IS - 3

ER -