TY - JOUR
T1 - Therapeutic drug monitoring of anti-epileptic drugs - a clinical verification of volumetric absorptive micro sampling
AU - Canisius, Thierry P. I. J. M.
AU - Soons, J. W. P. Hans
AU - Verschuure, Pauline
AU - Wammes-van der Heijden, Emmeke A.
AU - Rouhl, Rob P. W.
AU - Majoie, H. J. Marian
N1 - Publisher Copyright:
© 2020 2020 Walter de Gruyter GmbH, Berlin/Boston.
PY - 2020/5
Y1 - 2020/5
N2 - Background: Therapeutic drug monitoring (TDM) of antiepileptic drugs (AEDs) can serve as a valuable tool in optimising and individualising epilepsy treatment, especially in vulnerable groups such as pregnant women, the elderly and children. Unfortunately, TDM is often performed suboptimally due to limitations in blood collection. Therefore, we investigated volumetric absorptive micro sampling (VAMS) - a new home-sampling technique. We aimed to evaluate VAMS to determine and quantify the different AEDs and concentrations of 16 different AEDs in whole blood collected by VAMS.Methods: Patient blood samples (n = 138) were collected via venepunctures at the Academic Centre for Epileptology Kempenhaeghe. AED concentrations were determined, and these concentrations were used to compare the VAMS method (whole blood) with the conventional method (serum). In addition, the recovery was examined as well as the impact of haematocrit. Finally, AED-spiked blood was used to test the stability of the AEDs inside the micro-sampler devices over a period of time and whether temperature had an effect on the stability.Results: VAMS allows for an accurate detection of 16 different AEDs within 2 days after sampling. Deviation in recovery was less than 10% and high correlations were found between VAMS and conventional sampling. Moreover, haematocrit does not have an effect with values between 0.3 and 0.5 (L/L). Finally, although storage temperature of VAMS does affect some AEDs, most are unaffected.Conclusions: VAMS enables an accurate detection of a wide variety of AEDs within 2 days after sampling.
AB - Background: Therapeutic drug monitoring (TDM) of antiepileptic drugs (AEDs) can serve as a valuable tool in optimising and individualising epilepsy treatment, especially in vulnerable groups such as pregnant women, the elderly and children. Unfortunately, TDM is often performed suboptimally due to limitations in blood collection. Therefore, we investigated volumetric absorptive micro sampling (VAMS) - a new home-sampling technique. We aimed to evaluate VAMS to determine and quantify the different AEDs and concentrations of 16 different AEDs in whole blood collected by VAMS.Methods: Patient blood samples (n = 138) were collected via venepunctures at the Academic Centre for Epileptology Kempenhaeghe. AED concentrations were determined, and these concentrations were used to compare the VAMS method (whole blood) with the conventional method (serum). In addition, the recovery was examined as well as the impact of haematocrit. Finally, AED-spiked blood was used to test the stability of the AEDs inside the micro-sampler devices over a period of time and whether temperature had an effect on the stability.Results: VAMS allows for an accurate detection of 16 different AEDs within 2 days after sampling. Deviation in recovery was less than 10% and high correlations were found between VAMS and conventional sampling. Moreover, haematocrit does not have an effect with values between 0.3 and 0.5 (L/L). Finally, although storage temperature of VAMS does affect some AEDs, most are unaffected.Conclusions: VAMS enables an accurate detection of a wide variety of AEDs within 2 days after sampling.
KW - anti-epileptic drugs
KW - therapeutic drug monitoring
KW - volumetric absorptive micro sampling
KW - EPILEPSY
U2 - 10.1515/cclm-2019-0784
DO - 10.1515/cclm-2019-0784
M3 - Article
C2 - 32045349
SN - 1434-6621
VL - 58
SP - 828
EP - 835
JO - Clinical Chemistry and Laboratory Medicine
JF - Clinical Chemistry and Laboratory Medicine
IS - 5
ER -