Theranostic USPIO-Loaded Microbubbles for Mediating and Monitoring Blood-Brain Barrier Permeation

Twan Lammers*, Patrick Koczera, Stanley Fokong, Felix Gremse, Josef Ehling, Michael Vogt, Andrij Pich, Gert Storm, Marc van Zandvoort, Fabian Kiessling

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review


    Efficient and safe drug delivery across the blood-brain barrier (BBB) remains one of the major challenges of biomedical and (nano-) pharmaceutical research. Here, it is demonstrated that poly(butyl cyanoacrylate)-based microbubbles (MB), carrying ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles within their shell, can be used to mediate and monitor BBB permeation. Upon exposure to transcranial ultrasound pulses, USPIO-MB are destroyed, resulting in acoustic forces inducing vessel permeability. At the same time, USPIO are released from the MB shell, they extravasate across the permeabilized BBB and they accumulate in extravascular brain tissue, thereby providing non-invasive R-2(star)-based magnetic resonance imaging information on the extent of BBB opening. Quantitative changes in R-2(star) relaxometry are in good agreement with 2D and 3D microscopy results on the extravascular deposition of the macromolecular model drug fluorescein isothiocyanate (FITC)-dextran into the brain. Such theranostic materials and methods are considered to be useful for mediating and monitoring drug delivery across the BBB and for enabling safe and efficient treatment of CNS disorders.
    Original languageEnglish
    Pages (from-to)36-43
    JournalAdvanced Functional Materials
    Issue number1
    Publication statusPublished - 7 Jan 2015

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