Theobromine Does Not Affect Fasting and Postprandial HDL Cholesterol Efflux Capacity, While It Decreases Fasting miR-92a Levels in Humans

Charlotte P. J. Talbot, Ronald P. Mensink, Lotte Smolders, Virginie Bakeroot, Jogchum Plat*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

9 Citations (Web of Science)

Abstract

SCOPE: Chocolate consumption lowers cardiovascular disease risk, which might be attributed to the methylxanthine theobromine. These effects may be mediated through effects on HDL-mediated cholesterol efflux, which may be affected by microRNA (miRNA) levels in the HDL particles. Therefore, the aim of this study is to investigate effects of theobromine consumption on fasting and postprandial cholesterol efflux and miRNAs levels.

METHODS AND RESULTS: Thirty overweight and 14 obese healthy men and women participated in this randomized, double-blind crossover study. Participants consumed 500 mg d -1 of theobromine or placebo for 4 weeks. ABCA1-mediated cholesterol efflux was measured using J774 macrophages. MiRNAs levels (miR-92a, miR-223, miR-135a*) were quantified in apolipoprotein B-depleted serum. Theobromine consumption did not affect fasting and postprandial cholesterol efflux. Fasting miR-223 and miR-135a levels were unchanged, while miR-92a levels were decreased (-0.21; p < 0.05). The high-fat meal increased postprandial cholesterol efflux capacity (+4.3 percentage points; p ≤ 0.001), miR-92a (+1.21; p < 0.001), and miR-223 (+1.79; p < 0.001) levels, while a trend was found for miR-135a (+1.08; p = 0.06).

CONCLUSION: Theobromine did not improve fasting and postprandial ABCA1-mediated cholesterol efflux capacity, but decreased fasting miR-92a levels. High-fat meal intake increased postprandial cholesterol efflux and the three selected miRNAs levels.

Original languageEnglish
Article number1800027
Number of pages8
JournalMolecular Nutrition & Food Research
Volume62
Issue number13
DOIs
Publication statusPublished - Jul 2018

Keywords

  • cholesterol efflux capacity
  • high-density lipoprotein
  • microRNAs
  • postprandial
  • theobromine
  • ESTER TRANSFER PROTEIN
  • CARDIOVASCULAR-DISEASE
  • CIRCULATING MICRORNAS
  • POSTMENOPAUSAL WOMEN
  • INSULIN-RESISTANCE
  • GENE-EXPRESSION
  • SHORT-TERM
  • SERUM
  • COCOA
  • CONSUMPTION
  • Humans
  • Middle Aged
  • Cholesterol, HDL/metabolism
  • Male
  • Theobromine/administration & dosage
  • Postprandial Period
  • Female
  • Double-Blind Method
  • Cross-Over Studies
  • Fasting/metabolism
  • Animals
  • MicroRNAs/blood
  • ATP Binding Cassette Transporter 1/physiology
  • Aged
  • Mice

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