The sulfatase pathway as estrogen supply in endometrial cancer

K. M. C. Cornel; B. Delvoux; T. Saya; S. Xanthoulea; G. F. J. Konings; R. P. F. M. Kruitwagen; M. Y. Bongers; L. Kooreman; A. Romano

doi:10.1016/j.steroids.2018.09.002

The sulfatase pathway as estrogen supply in endometrial cancer

K. M. C. Cornel, B. Delvoux, T. Saya, S. Xanthoulea, G. F. J. Konings, R. P. F. M. Kruitwagen, M. Y. Bongers, L. Kooreman, A. Romano^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

6 Citations (Web of Science)

Abstract

Objective: Contradictory results are reported about the level of steroid sulfatase (STS), estrogen sulfotransferase (SULT1E1; together, the sulfatase pathway) and aromatase (CYP19A1) in endometrial cancer (EC). The aim of this study was to explore the levels of these enzymes in a well-characterized cohort of EC patients and postmenopausal controls.

Materials and Methods: Endometrial tissues from 31 EC patients (21 grade 1 and 10 grade 2-3) and 19 postmenopausal controls were collected. Levels of mRNA (RT-qPCR) and protein (immunohistochemistry) were determined. STS enzyme activity was measured by HPLC, whereas SULT1E1 enzyme activity was determined using a novel method based on liquid chromatography-mass spectrometry (LC-MS/MS).

Results: No significant differences in STS, SULT1E1 mRNA or protein levels and STS:SULT1E1 ratio were found. STS enzyme activity and STS:SULT1E1 activity ratio were significantly decreased in ECs compared with controls. CYP19A1 mRNA levels were lower in ECs than in controls.

Conclusion: A novel highly sensitive and accurate protocol to assess SULT1E1 activity is presented. STS enzyme activity and the STS:SULT1E1 activity ratio seem to be lower in ECs than in controls. STS is an important route for estrogen supply in endometrial cells.

Original language	English
Pages (from-to)	45-52
Number of pages	8
Journal	Steroids
Volume	139
DOIs	https://doi.org/10.1016/j.steroids.2018.09.002
Publication status	Published - Nov 2018

Keywords

Steroid sulfatase
Estrogen sulfotransferase
Endometrial cancer
Intracrinology
Estrogen metabolism
STEROID SULFATASE
17-BETA-HYDROXYSTEROID-DEHYDROGENASE TYPE-1
BREAST-CANCER
INHIBITOR IROSUSTAT
PHASE-II
RISK
17-BETA-ESTRADIOL
SULFOTRANSFERASE
METABOLISM
EXPRESSION

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10.1016/j.steroids.2018.09.002

Cite this

@article{5fe36f423d6641dfaeedba6e7bde30f1,

title = "The sulfatase pathway as estrogen supply in endometrial cancer",

abstract = "Objective: Contradictory results are reported about the level of steroid sulfatase (STS), estrogen sulfotransferase (SULT1E1; together, the sulfatase pathway) and aromatase (CYP19A1) in endometrial cancer (EC). The aim of this study was to explore the levels of these enzymes in a well-characterized cohort of EC patients and postmenopausal controls.Materials and Methods: Endometrial tissues from 31 EC patients (21 grade 1 and 10 grade 2-3) and 19 postmenopausal controls were collected. Levels of mRNA (RT-qPCR) and protein (immunohistochemistry) were determined. STS enzyme activity was measured by HPLC, whereas SULT1E1 enzyme activity was determined using a novel method based on liquid chromatography-mass spectrometry (LC-MS/MS).Results: No significant differences in STS, SULT1E1 mRNA or protein levels and STS:SULT1E1 ratio were found. STS enzyme activity and STS:SULT1E1 activity ratio were significantly decreased in ECs compared with controls. CYP19A1 mRNA levels were lower in ECs than in controls.Conclusion: A novel highly sensitive and accurate protocol to assess SULT1E1 activity is presented. STS enzyme activity and the STS:SULT1E1 activity ratio seem to be lower in ECs than in controls. STS is an important route for estrogen supply in endometrial cells.",

keywords = "Steroid sulfatase, Estrogen sulfotransferase, Endometrial cancer, Intracrinology, Estrogen metabolism, STEROID SULFATASE, 17-BETA-HYDROXYSTEROID-DEHYDROGENASE TYPE-1, BREAST-CANCER, INHIBITOR IROSUSTAT, PHASE-II, RISK, 17-BETA-ESTRADIOL, SULFOTRANSFERASE, METABOLISM, EXPRESSION",

author = "Cornel, {K. M. C.} and B. Delvoux and T. Saya and S. Xanthoulea and Konings, {G. F. J.} and Kruitwagen, {R. P. F. M.} and Bongers, {M. Y.} and L. Kooreman and A. Romano",

year = "2018",

month = nov,

doi = "10.1016/j.steroids.2018.09.002",

language = "English",

volume = "139",

pages = "45--52",

journal = "Steroids",

issn = "0039-128X",

publisher = "Elsevier Science",

}

TY - JOUR

T1 - The sulfatase pathway as estrogen supply in endometrial cancer

AU - Cornel, K. M. C.

AU - Delvoux, B.

AU - Saya, T.

AU - Xanthoulea, S.

AU - Konings, G. F. J.

AU - Kruitwagen, R. P. F. M.

AU - Bongers, M. Y.

AU - Kooreman, L.

AU - Romano, A.

PY - 2018/11

Y1 - 2018/11

N2 - Objective: Contradictory results are reported about the level of steroid sulfatase (STS), estrogen sulfotransferase (SULT1E1; together, the sulfatase pathway) and aromatase (CYP19A1) in endometrial cancer (EC). The aim of this study was to explore the levels of these enzymes in a well-characterized cohort of EC patients and postmenopausal controls.Materials and Methods: Endometrial tissues from 31 EC patients (21 grade 1 and 10 grade 2-3) and 19 postmenopausal controls were collected. Levels of mRNA (RT-qPCR) and protein (immunohistochemistry) were determined. STS enzyme activity was measured by HPLC, whereas SULT1E1 enzyme activity was determined using a novel method based on liquid chromatography-mass spectrometry (LC-MS/MS).Results: No significant differences in STS, SULT1E1 mRNA or protein levels and STS:SULT1E1 ratio were found. STS enzyme activity and STS:SULT1E1 activity ratio were significantly decreased in ECs compared with controls. CYP19A1 mRNA levels were lower in ECs than in controls.Conclusion: A novel highly sensitive and accurate protocol to assess SULT1E1 activity is presented. STS enzyme activity and the STS:SULT1E1 activity ratio seem to be lower in ECs than in controls. STS is an important route for estrogen supply in endometrial cells.

AB - Objective: Contradictory results are reported about the level of steroid sulfatase (STS), estrogen sulfotransferase (SULT1E1; together, the sulfatase pathway) and aromatase (CYP19A1) in endometrial cancer (EC). The aim of this study was to explore the levels of these enzymes in a well-characterized cohort of EC patients and postmenopausal controls.Materials and Methods: Endometrial tissues from 31 EC patients (21 grade 1 and 10 grade 2-3) and 19 postmenopausal controls were collected. Levels of mRNA (RT-qPCR) and protein (immunohistochemistry) were determined. STS enzyme activity was measured by HPLC, whereas SULT1E1 enzyme activity was determined using a novel method based on liquid chromatography-mass spectrometry (LC-MS/MS).Results: No significant differences in STS, SULT1E1 mRNA or protein levels and STS:SULT1E1 ratio were found. STS enzyme activity and STS:SULT1E1 activity ratio were significantly decreased in ECs compared with controls. CYP19A1 mRNA levels were lower in ECs than in controls.Conclusion: A novel highly sensitive and accurate protocol to assess SULT1E1 activity is presented. STS enzyme activity and the STS:SULT1E1 activity ratio seem to be lower in ECs than in controls. STS is an important route for estrogen supply in endometrial cells.

KW - Steroid sulfatase

KW - Estrogen sulfotransferase

KW - Endometrial cancer

KW - Intracrinology

KW - Estrogen metabolism

KW - STEROID SULFATASE

KW - 17-BETA-HYDROXYSTEROID-DEHYDROGENASE TYPE-1

KW - BREAST-CANCER

KW - INHIBITOR IROSUSTAT

KW - PHASE-II

KW - RISK

KW - 17-BETA-ESTRADIOL

KW - SULFOTRANSFERASE

KW - METABOLISM

KW - EXPRESSION

U2 - 10.1016/j.steroids.2018.09.002

DO - 10.1016/j.steroids.2018.09.002

M3 - Article

C2 - 30217785

VL - 139

SP - 45

EP - 52

JO - Steroids

JF - Steroids

SN - 0039-128X

ER -