The sulfatase pathway as estrogen supply in endometrial cancer

K. M. C. Cornel, B. Delvoux, T. Saya, S. Xanthoulea, G. F. J. Konings, R. P. F. M. Kruitwagen, M. Y. Bongers, L. Kooreman, A. Romano*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Web of Science)

Abstract

Objective: Contradictory results are reported about the level of steroid sulfatase (STS), estrogen sulfotransferase (SULT1E1; together, the sulfatase pathway) and aromatase (CYP19A1) in endometrial cancer (EC). The aim of this study was to explore the levels of these enzymes in a well-characterized cohort of EC patients and postmenopausal controls.

Materials and Methods: Endometrial tissues from 31 EC patients (21 grade 1 and 10 grade 2-3) and 19 postmenopausal controls were collected. Levels of mRNA (RT-qPCR) and protein (immunohistochemistry) were determined. STS enzyme activity was measured by HPLC, whereas SULT1E1 enzyme activity was determined using a novel method based on liquid chromatography-mass spectrometry (LC-MS/MS).

Results: No significant differences in STS, SULT1E1 mRNA or protein levels and STS:SULT1E1 ratio were found. STS enzyme activity and STS:SULT1E1 activity ratio were significantly decreased in ECs compared with controls. CYP19A1 mRNA levels were lower in ECs than in controls.

Conclusion: A novel highly sensitive and accurate protocol to assess SULT1E1 activity is presented. STS enzyme activity and the STS:SULT1E1 activity ratio seem to be lower in ECs than in controls. STS is an important route for estrogen supply in endometrial cells.

Original languageEnglish
Pages (from-to)45-52
Number of pages8
JournalSteroids
Volume139
DOIs
Publication statusPublished - Nov 2018

Keywords

  • Steroid sulfatase
  • Estrogen sulfotransferase
  • Endometrial cancer
  • Intracrinology
  • Estrogen metabolism
  • STEROID SULFATASE
  • 17-BETA-HYDROXYSTEROID-DEHYDROGENASE TYPE-1
  • BREAST-CANCER
  • INHIBITOR IROSUSTAT
  • PHASE-II
  • RISK
  • 17-BETA-ESTRADIOL
  • SULFOTRANSFERASE
  • METABOLISM
  • EXPRESSION

Cite this