Abstract
Purpose: To investigate whether the rs12569232 SNP association with Vogt-Koyanagi-Harada disease and Behcet's disease is mediated by regulation of Linc00467 expression. Methods: The expression of linc00467 was detected by real-time PCR. Adenovirus carrying the linc00467 was transduced into CD4(+)T cells and the effect on cell viability was measured by the CCK-8 test. Human proteome microarray and starBase 2.0 were used to identify the binding proteins of linc00467 and RNA Immunoprecipitation (RIP) was used to confirm the identity of bound proteins. Results: The rs12569232 was associated with the expression of linc00467. The expression of linc00467 was up-regulated in PBMCs and CD4(+)T cells from VKH disease and BD patients. Over-expression of linc00467 increased cell viability of CD4(+)T cells. HUR was the common binding protein identified by the two methods and confirmed by RIP. Conclusions: The rs12569232 association with VKH disease and BD may be mediated via regulating the expression of linc00467.
Original language | English |
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Pages (from-to) | 1464-1470 |
Number of pages | 7 |
Journal | Ocular Immunology and Inflammation |
Volume | 29 |
Issue number | 7-8 |
Early online date | 10 May 2020 |
DOIs | |
Publication status | Published - 17 Nov 2021 |
Keywords
- behcet's disease
- cd4(+)t cells
- hur
- insights
- lincrna
- single nucleotide polymorphism
- vogt-koyanagi-harada disease
- Vogt-Koyanagi-Harada disease
- HUR
- Cd4(+)T cells
- Behcet's disease
- lincRNA
- INSIGHTS