The role of microstructured and interconnected pore channels in a collagen-based nerve guide on axonal regeneration in peripheral nerves

Ahmet Bozkurt; Franz Lassner; Dan O'Dey; Ronald Deumens; Arne Boecker; Tilman Schwendt; Christoph Janzen; Christoph V. Suschek; Rene Tolba; Eiji Kobayashi; Bernd Sellhaus; S. Tholl; Lizette Eummelen; Frank Schuegner; Leon Olde Damink; Joachim Weis; Gary A. Brook; Norbert Pallua

doi:10.1016/j.biomaterials.2011.10.069

The role of microstructured and interconnected pore channels in a collagen-based nerve guide on axonal regeneration in peripheral nerves

Ahmet Bozkurt^*, Franz Lassner, Dan O'Dey, Ronald Deumens, Arne Boecker, Tilman Schwendt, Christoph Janzen, Christoph V. Suschek, Rene Tolba, Eiji Kobayashi, Bernd Sellhaus, S. Tholl, Lizette Eummelen, Frank Schuegner, Leon Olde Damink, Joachim Weis, Gary A. Brook, Norbert Pallua

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

The use of bioengineered nerve guides as alternatives for autologous nerve transplantation (ANT) is a promising strategy for the repair of peripheral nerve defects. In the present investigation, we present a collagen-based micro-structured nerve guide (Perimaix) for the repair of 2 cm rat sciatic nerve defects. Perimaix is an open-porous biodegradable nerve guide containing continuous, longitudinally orientated channels for orientated nerve growth. The effects of these nerve guides on axon regeneration by six weeks after implantation have been compared with those of ANT. Investigation of the regenerated sciatic nerve indicated that Perimaix strongly supported directed axon regeneration. When seeded with cultivated rat Schwann cells (SC), the Perimaix nerve guide was found to be almost as supportive of axon regeneration as ANT. The use of SC from transgenic green-fluorescent-protein (GFP) rats allowed us to detect the viability of donor SC at 1 week and 6 weeks after transplantation. The GFP-positive SC were aligned in a columnar fashion within the longitudinally orientated micro-channels. This cellular arrangement was not only observed prior to implantation, but also at one week and 6 weeks after implantation. It may be concluded that Perimaix nerve guides hold great promise for the repair of peripheral nerve defects.

Original language	English
Pages (from-to)	1363-1375
Journal	Biomaterials
Volume	33
Issue number	5
DOIs	https://doi.org/10.1016/j.biomaterials.2011.10.069
Publication status	Published - Feb 2012

Keywords

Nerve regeneration
Schwann cells
Scaffold
Neurotmesis
Sciatic nerve
Bands of Bungner

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10.1016/j.biomaterials.2011.10.069

Cite this

Bozkurt, A., Lassner, F., O'Dey, D., Deumens, R., Boecker, A., Schwendt, T., Janzen, C., Suschek, C. V., Tolba, R., Kobayashi, E., Sellhaus, B., Tholl, S., Eummelen, L., Schuegner, F., Damink, L. O., Weis, J., Brook, G. A., & Pallua, N. (2012). The role of microstructured and interconnected pore channels in a collagen-based nerve guide on axonal regeneration in peripheral nerves. Biomaterials, 33(5), 1363-1375. https://doi.org/10.1016/j.biomaterials.2011.10.069

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title = "The role of microstructured and interconnected pore channels in a collagen-based nerve guide on axonal regeneration in peripheral nerves",

abstract = "The use of bioengineered nerve guides as alternatives for autologous nerve transplantation (ANT) is a promising strategy for the repair of peripheral nerve defects. In the present investigation, we present a collagen-based micro-structured nerve guide (Perimaix) for the repair of 2 cm rat sciatic nerve defects. Perimaix is an open-porous biodegradable nerve guide containing continuous, longitudinally orientated channels for orientated nerve growth. The effects of these nerve guides on axon regeneration by six weeks after implantation have been compared with those of ANT. Investigation of the regenerated sciatic nerve indicated that Perimaix strongly supported directed axon regeneration. When seeded with cultivated rat Schwann cells (SC), the Perimaix nerve guide was found to be almost as supportive of axon regeneration as ANT. The use of SC from transgenic green-fluorescent-protein (GFP) rats allowed us to detect the viability of donor SC at 1 week and 6 weeks after transplantation. The GFP-positive SC were aligned in a columnar fashion within the longitudinally orientated micro-channels. This cellular arrangement was not only observed prior to implantation, but also at one week and 6 weeks after implantation. It may be concluded that Perimaix nerve guides hold great promise for the repair of peripheral nerve defects. ",

keywords = "Nerve regeneration, Schwann cells, Scaffold, Neurotmesis, Sciatic nerve, Bands of Bungner",

author = "Ahmet Bozkurt and Franz Lassner and Dan O'Dey and Ronald Deumens and Arne Boecker and Tilman Schwendt and Christoph Janzen and Suschek, {Christoph V.} and Rene Tolba and Eiji Kobayashi and Bernd Sellhaus and S. Tholl and Lizette Eummelen and Frank Schuegner and Damink, {Leon Olde} and Joachim Weis and Brook, {Gary A.} and Norbert Pallua",

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doi = "10.1016/j.biomaterials.2011.10.069",

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Bozkurt, A, Lassner, F, O'Dey, D, Deumens, R, Boecker, A, Schwendt, T, Janzen, C, Suschek, CV, Tolba, R, Kobayashi, E, Sellhaus, B, Tholl, S, Eummelen, L, Schuegner, F, Damink, LO, Weis, J, Brook, GA & Pallua, N 2012, 'The role of microstructured and interconnected pore channels in a collagen-based nerve guide on axonal regeneration in peripheral nerves', Biomaterials, vol. 33, no. 5, pp. 1363-1375. https://doi.org/10.1016/j.biomaterials.2011.10.069

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T1 - The role of microstructured and interconnected pore channels in a collagen-based nerve guide on axonal regeneration in peripheral nerves

AU - Bozkurt, Ahmet

AU - Lassner, Franz

AU - O'Dey, Dan

AU - Deumens, Ronald

AU - Boecker, Arne

AU - Schwendt, Tilman

AU - Janzen, Christoph

AU - Suschek, Christoph V.

AU - Tolba, Rene

AU - Kobayashi, Eiji

AU - Sellhaus, Bernd

AU - Tholl, S.

AU - Eummelen, Lizette

AU - Schuegner, Frank

AU - Damink, Leon Olde

AU - Weis, Joachim

AU - Brook, Gary A.

AU - Pallua, Norbert

PY - 2012/2

Y1 - 2012/2

N2 - The use of bioengineered nerve guides as alternatives for autologous nerve transplantation (ANT) is a promising strategy for the repair of peripheral nerve defects. In the present investigation, we present a collagen-based micro-structured nerve guide (Perimaix) for the repair of 2 cm rat sciatic nerve defects. Perimaix is an open-porous biodegradable nerve guide containing continuous, longitudinally orientated channels for orientated nerve growth. The effects of these nerve guides on axon regeneration by six weeks after implantation have been compared with those of ANT. Investigation of the regenerated sciatic nerve indicated that Perimaix strongly supported directed axon regeneration. When seeded with cultivated rat Schwann cells (SC), the Perimaix nerve guide was found to be almost as supportive of axon regeneration as ANT. The use of SC from transgenic green-fluorescent-protein (GFP) rats allowed us to detect the viability of donor SC at 1 week and 6 weeks after transplantation. The GFP-positive SC were aligned in a columnar fashion within the longitudinally orientated micro-channels. This cellular arrangement was not only observed prior to implantation, but also at one week and 6 weeks after implantation. It may be concluded that Perimaix nerve guides hold great promise for the repair of peripheral nerve defects.

AB - The use of bioengineered nerve guides as alternatives for autologous nerve transplantation (ANT) is a promising strategy for the repair of peripheral nerve defects. In the present investigation, we present a collagen-based micro-structured nerve guide (Perimaix) for the repair of 2 cm rat sciatic nerve defects. Perimaix is an open-porous biodegradable nerve guide containing continuous, longitudinally orientated channels for orientated nerve growth. The effects of these nerve guides on axon regeneration by six weeks after implantation have been compared with those of ANT. Investigation of the regenerated sciatic nerve indicated that Perimaix strongly supported directed axon regeneration. When seeded with cultivated rat Schwann cells (SC), the Perimaix nerve guide was found to be almost as supportive of axon regeneration as ANT. The use of SC from transgenic green-fluorescent-protein (GFP) rats allowed us to detect the viability of donor SC at 1 week and 6 weeks after transplantation. The GFP-positive SC were aligned in a columnar fashion within the longitudinally orientated micro-channels. This cellular arrangement was not only observed prior to implantation, but also at one week and 6 weeks after implantation. It may be concluded that Perimaix nerve guides hold great promise for the repair of peripheral nerve defects.

KW - Nerve regeneration

KW - Schwann cells

KW - Scaffold

KW - Neurotmesis

KW - Sciatic nerve

KW - Bands of Bungner

U2 - 10.1016/j.biomaterials.2011.10.069

DO - 10.1016/j.biomaterials.2011.10.069

M3 - Article

C2 - 22082619

SN - 0142-9612

VL - 33

SP - 1363

EP - 1375

JO - Biomaterials

JF - Biomaterials

IS - 5

ER -