The Relationship Between Prostaglandin E Receptor 1 and Cyclooxygenase I Expression in Guinea Pig Bladder Interstitial Cells: Proposition of a Signal Propagation System

Mohammad Sajjad Rahnama'i*, Stefan G. G. de Wachter, Gommert A. van Koeveringe, Philip E. V. A. Van Kerrebroeck, Jan de Vente, James I. Gillespie

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Purpose: We explored the structural relationship between enzymes producing prostaglandin (cyclooxygenase I) and 1 of the receptor families that respond to prostaglandin (prostaglandin E receptor 1) in the bladder muscle. Materials and Methods: Nine male guinea pigs were sacrificed by cervical dislocation. Bladders were removed and fixed in 4% paraformaldehyde in phosphate buffered saline. Frozen sections (10 mu m) were cut and stained with antibodies to prostaglandin E receptor type 1, cyclooxygenase I and vimentin. Results: Prostaglandin E receptors 1 was identified on smooth muscle cells, and vimentin positive surface muscle and intramuscular interstitial cells. Muscle staining was less intense than on interstitial cells and had a punctuate appearance. Prostaglandin E receptor 1 expression on interstitial cells was highly localized. Discrete regions of intense staining were noted on interstitial cell processes. Cyclooxygenase I was also expressed in muscle interstitial cells. Cyclooxygenase I positive interstitial cells were more prevalent in the muscle bundles of the inner muscle than in the outer muscle layers. Cyclooxygenase I staining was noted on discrete regions of the cell or cell processes. Double staining with prostaglandin E receptor 1 and cyclooxygenase I suggested that cell regions expressing the former are different from those expressing the latter. Conclusions: The discovered arrangement of prostaglandin E receptor 1 and cyclooxygenase I may have the potential to facilitate the propagation of signals in the interstitial cell network. Such a signaling system may have a role in coordinating events, as in bladder pathology, facilitating the global coordinated changes associated with bladder wall remodeling.
Original languageEnglish
Pages (from-to)315-322
JournalJournal of Urology
Volume185
Issue number1
DOIs
Publication statusPublished - Jan 2011

Keywords

  • urinary bladder
  • prostaglandin-endoperoxide synthases
  • intracellular signaling peptides and proteins
  • muscles
  • guinea pigs

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