The prevalence of the Staphylococcus aureus tst gene among community- and hospital-acquired strains and isolates from Wegener's Granulomatosis patients

R.H.C.A. Deurenberg; R.F. Nieuwenhuis; C.C. Driessen; N. London; F.R. Stassen; F.H. van Tiel; E.E. Stobberingh; C. Vink

doi:10.1016/j.femsle.2005.03.002

The prevalence of the Staphylococcus aureus tst gene among community- and hospital-acquired strains and isolates from Wegener's Granulomatosis patients

R.H.C.A. Deurenberg, R.F. Nieuwenhuis, C.C. Driessen, N. London, F.R. Stassen, F.H. van Tiel, E.E. Stobberingh, C. Vink^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

To allow rapid identification of toxic shock syndrome toxin-1 (TSST-1)-producing Staphylococcus aureus strains, a real-time PCR assay for the detection of the tst gene, which encodes TSST-1, was developed. The assay was applied to S. aureus isolates from patients with Wegener's Granulomatosis (WG), as well as isolates that were classified as either community- (CA) or hospital-acquired (HA). No significant difference in the percentage of tst-positive strains was observed between isolates from WG patients and CA isolates (24% and 25%, respectively). In contrast, only 14% of the HA isolates were tst-positive (p<0.05). Investigation of the clonal relationship between tst-positive CA and HA strains could indicated the recent emergence of a virulent S. aureus clone in the community.

Original language	English
Pages (from-to)	185-189
Journal	Fems Microbiology Letters
Volume	245
Issue number	1
DOIs	https://doi.org/10.1016/j.femsle.2005.03.002
Publication status	Published - 1 Jan 2005

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10.1016/j.femsle.2005.03.002

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Deurenberg, R. H. C. A., Nieuwenhuis, R. F., Driessen, C. C., London, N., Stassen, F. R., van Tiel, F. H., Stobberingh, E. E., & Vink, C. (2005). The prevalence of the Staphylococcus aureus tst gene among community- and hospital-acquired strains and isolates from Wegener's Granulomatosis patients. Fems Microbiology Letters, 245(1), 185-189. https://doi.org/10.1016/j.femsle.2005.03.002

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title = "The prevalence of the Staphylococcus aureus tst gene among community- and hospital-acquired strains and isolates from Wegener's Granulomatosis patients",

abstract = "To allow rapid identification of toxic shock syndrome toxin-1 (TSST-1)-producing Staphylococcus aureus strains, a real-time PCR assay for the detection of the tst gene, which encodes TSST-1, was developed. The assay was applied to S. aureus isolates from patients with Wegener's Granulomatosis (WG), as well as isolates that were classified as either community- (CA) or hospital-acquired (HA). No significant difference in the percentage of tst-positive strains was observed between isolates from WG patients and CA isolates (24% and 25%, respectively). In contrast, only 14% of the HA isolates were tst-positive (p<0.05). Investigation of the clonal relationship between tst-positive CA and HA strains could indicated the recent emergence of a virulent S. aureus clone in the community.",

author = "R.H.C.A. Deurenberg and R.F. Nieuwenhuis and C.C. Driessen and N. London and F.R. Stassen and {van Tiel}, F.H. and E.E. Stobberingh and C. Vink",

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Deurenberg, RHCA, Nieuwenhuis, RF, Driessen, CC, London, N, Stassen, FR, van Tiel, FH, Stobberingh, EE & Vink, C 2005, 'The prevalence of the Staphylococcus aureus tst gene among community- and hospital-acquired strains and isolates from Wegener's Granulomatosis patients', Fems Microbiology Letters, vol. 245, no. 1, pp. 185-189. https://doi.org/10.1016/j.femsle.2005.03.002

The prevalence of the Staphylococcus aureus tst gene among community- and hospital-acquired strains and isolates from Wegener's Granulomatosis patients. / Deurenberg, R.H.C.A.; Nieuwenhuis, R.F.; Driessen, C.C. et al.
In: Fems Microbiology Letters, Vol. 245, No. 1, 01.01.2005, p. 185-189.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - The prevalence of the Staphylococcus aureus tst gene among community- and hospital-acquired strains and isolates from Wegener's Granulomatosis patients

AU - Deurenberg, R.H.C.A.

AU - Nieuwenhuis, R.F.

AU - Driessen, C.C.

AU - London, N.

AU - Stassen, F.R.

AU - van Tiel, F.H.

AU - Stobberingh, E.E.

AU - Vink, C.

PY - 2005/1/1

Y1 - 2005/1/1

N2 - To allow rapid identification of toxic shock syndrome toxin-1 (TSST-1)-producing Staphylococcus aureus strains, a real-time PCR assay for the detection of the tst gene, which encodes TSST-1, was developed. The assay was applied to S. aureus isolates from patients with Wegener's Granulomatosis (WG), as well as isolates that were classified as either community- (CA) or hospital-acquired (HA). No significant difference in the percentage of tst-positive strains was observed between isolates from WG patients and CA isolates (24% and 25%, respectively). In contrast, only 14% of the HA isolates were tst-positive (p<0.05). Investigation of the clonal relationship between tst-positive CA and HA strains could indicated the recent emergence of a virulent S. aureus clone in the community.

AB - To allow rapid identification of toxic shock syndrome toxin-1 (TSST-1)-producing Staphylococcus aureus strains, a real-time PCR assay for the detection of the tst gene, which encodes TSST-1, was developed. The assay was applied to S. aureus isolates from patients with Wegener's Granulomatosis (WG), as well as isolates that were classified as either community- (CA) or hospital-acquired (HA). No significant difference in the percentage of tst-positive strains was observed between isolates from WG patients and CA isolates (24% and 25%, respectively). In contrast, only 14% of the HA isolates were tst-positive (p<0.05). Investigation of the clonal relationship between tst-positive CA and HA strains could indicated the recent emergence of a virulent S. aureus clone in the community.

U2 - 10.1016/j.femsle.2005.03.002

DO - 10.1016/j.femsle.2005.03.002

M3 - Article

SN - 0378-1097

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JO - Fems Microbiology Letters

JF - Fems Microbiology Letters

IS - 1

ER -

The prevalence of the Staphylococcus aureus tst gene among community- and hospital-acquired strains and isolates from Wegener's Granulomatosis patients

Abstract

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