The prevalence and transcriptional activity of the mucosal microbiota of ulcerative colitis patients

Aina E.Fossum Moen; Jonas Christoffer Lindstrøm; Tone Møller Tannæs; Simen Vatn; Petr Ricanek; Morten H. Vatn; Jørgen Jahnsen; Anna B. Frengen; Fredrik A. Dahl; Panpan You; Janne Sølvernes; Gunn S. Ekeland; Trond E. Detlie; Christine Olbjørn; Kate R. O’Leary; Nicholas T. Ventham; Nicholas A. Kennedy; Rahul Kalla; Alex Adams; Hazel E. Drummond; Ray Boyapati; Elaine R. Nimmo; David C. Wilson; Jack Satsangi; Simon C. Heath; Marta Gut; Angelika Merkel; Monica Bayes; Ivo G. Gut; Åsa V. Keita; Johan D. Söderholm; Henrik Hjortswang; Adam Carstens; Daniel Bergemalm; Jonas Halfvarson; Erik Andersson; Mårten Lindqvist; Dirk Repsilber; Marieke Pierik; Daisy Jonkers; Fernando Gomollón; Mauro D’Amato; Leif Törkvist; Fredrik Hjelm; Mats Gullberg; Niklas Nordberg; Anette Ocklind; Erik Pettersson; Daniel Ekman; Mikael Sundell; IBD Character Consortium

doi:10.1038/s41598-018-35243-4

The prevalence and transcriptional activity of the mucosal microbiota of ulcerative colitis patients

Aina E.Fossum Moen, Jonas Christoffer Lindstrøm, Tone Møller Tannæs^*, Simen Vatn, Petr Ricanek, Morten H. Vatn, Jørgen Jahnsen, Anna B. Frengen, Fredrik A. Dahl, Panpan You, Janne Sølvernes, Gunn S. Ekeland, Trond E. Detlie, Christine Olbjørn, Kate R. O’Leary, Nicholas T. Ventham, Nicholas A. Kennedy, Rahul Kalla, Alex Adams, Hazel E. DrummondRay Boyapati, Elaine R. Nimmo, David C. Wilson, Jack Satsangi, Simon C. Heath, Marta Gut, Angelika Merkel, Monica Bayes, Ivo G. Gut, Åsa V. Keita, Johan D. Söderholm, Henrik Hjortswang, Adam Carstens, Daniel Bergemalm, Jonas Halfvarson, Erik Andersson, Mårten Lindqvist, Dirk Repsilber, Marieke Pierik, Daisy Jonkers, Fernando Gomollón, Mauro D’Amato, Leif Törkvist, Fredrik Hjelm, Mats Gullberg, Niklas Nordberg, Anette Ocklind, Erik Pettersson, Daniel Ekman, Mikael Sundell, IBD Character Consortium

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Active microbes likely have larger impact on gut health status compared to inactive or dormant microbes. We investigate the composition of active and total mucosal microbiota of treatment-naïve ulcerative colitis (UC) patients to determine the microbial picture at the start-up phase of disease, using both a 16S rRNA transcript and gene amplicon sequencing. DNA and RNA were isolated from the same mucosal colonic biopsies. Our aim was to identify active microbial members of the microbiota in early stages of disease and reveal which members are present, but do not act as major players. We demonstrated differences in active and total microbiota of UC patients when comparing inflamed to non-inflamed tissue. Several taxa, among them the Proteobacteria phyla and families therein, revealed lower transcriptional activity despite a high presence. The Bifidobacteriaceae family of the Actinobacteria phylum showed lower abundance in the active microbiota, although no difference in presence was detected. The most abundant microbiota members of the inflamed tissue in UC patients were not the most active. Knowledge of active members of microbiota in UC patients could enhance our understanding of disease etiology. The active microbial community composition did not deviate from the total when comparing UC patients to non-IBD controls.

Original language	English
Article number	17278
Journal	Scientific Reports
Volume	8
Issue number	1
DOIs	https://doi.org/10.1038/s41598-018-35243-4
Publication status	Published - 1 Dec 2018

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Moen, A. E. F., Lindstrøm, J. C., Tannæs, T. M., Vatn, S., Ricanek, P., Vatn, M. H., Jahnsen, J., Frengen, A. B., Dahl, F. A., You, P., Sølvernes, J., Ekeland, G. S., Detlie, T. E., Olbjørn, C., O’Leary, K. R., Ventham, N. T., Kennedy, N. A., Kalla, R., Adams, A., ... IBD Character Consortium (2018). The prevalence and transcriptional activity of the mucosal microbiota of ulcerative colitis patients. Scientific Reports, 8(1), Article 17278. https://doi.org/10.1038/s41598-018-35243-4

@article{59840dfd4a4441c8b8d03be46398c483,

title = "The prevalence and transcriptional activity of the mucosal microbiota of ulcerative colitis patients",

abstract = "Active microbes likely have larger impact on gut health status compared to inactive or dormant microbes. We investigate the composition of active and total mucosal microbiota of treatment-na{\"i}ve ulcerative colitis (UC) patients to determine the microbial picture at the start-up phase of disease, using both a 16S rRNA transcript and gene amplicon sequencing. DNA and RNA were isolated from the same mucosal colonic biopsies. Our aim was to identify active microbial members of the microbiota in early stages of disease and reveal which members are present, but do not act as major players. We demonstrated differences in active and total microbiota of UC patients when comparing inflamed to non-inflamed tissue. Several taxa, among them the Proteobacteria phyla and families therein, revealed lower transcriptional activity despite a high presence. The Bifidobacteriaceae family of the Actinobacteria phylum showed lower abundance in the active microbiota, although no difference in presence was detected. The most abundant microbiota members of the inflamed tissue in UC patients were not the most active. Knowledge of active members of microbiota in UC patients could enhance our understanding of disease etiology. The active microbial community composition did not deviate from the total when comparing UC patients to non-IBD controls.",

author = "Moen, {Aina E.Fossum} and Lindstr{\o}m, {Jonas Christoffer} and Tann{\ae}s, {Tone M{\o}ller} and Simen Vatn and Petr Ricanek and Vatn, {Morten H.} and J{\o}rgen Jahnsen and Frengen, {Anna B.} and Dahl, {Fredrik A.} and Panpan You and Janne S{\o}lvernes and Ekeland, {Gunn S.} and Detlie, {Trond E.} and Christine Olbj{\o}rn and O{\textquoteright}Leary, {Kate R.} and Ventham, {Nicholas T.} and Kennedy, {Nicholas A.} and Rahul Kalla and Alex Adams and Drummond, {Hazel E.} and Ray Boyapati and Nimmo, {Elaine R.} and Wilson, {David C.} and Jack Satsangi and Heath, {Simon C.} and Marta Gut and Angelika Merkel and Monica Bayes and Gut, {Ivo G.} and Keita, {{\AA}sa V.} and S{\"o}derholm, {Johan D.} and Henrik Hjortswang and Adam Carstens and Daniel Bergemalm and Jonas Halfvarson and Erik Andersson and M{\aa}rten Lindqvist and Dirk Repsilber and Marieke Pierik and Daisy Jonkers and Fernando Gomoll{\'o}n and Mauro D{\textquoteright}Amato and Leif T{\"o}rkvist and Fredrik Hjelm and Mats Gullberg and Niklas Nordberg and Anette Ocklind and Erik Pettersson and Daniel Ekman and Mikael Sundell and {IBD Character Consortium}",

note = "Funding Information: The project was funded by the EU study IBD-Character (EU ref no 305676). We want to thank Engineer Sumana Kalyanasundaram, Institute of Informatics, University of Oslo, Norway for the bioinformatical help during the startup phase of the project. European IBD Character Consortium, FP7, Grant Agreement Number: 305676. Publisher Copyright: {\textcopyright} 2018, The Author(s).",

year = "2018",

month = dec,

day = "1",

doi = "10.1038/s41598-018-35243-4",

language = "English",

volume = "8",

journal = "Scientific Reports",

issn = "2045-2322",

publisher = "Nature Publishing Group",

number = "1",

}

Moen, AEF, Lindstrøm, JC, Tannæs, TM, Vatn, S, Ricanek, P, Vatn, MH, Jahnsen, J, Frengen, AB, Dahl, FA, You, P, Sølvernes, J, Ekeland, GS, Detlie, TE, Olbjørn, C, O’Leary, KR, Ventham, NT, Kennedy, NA, Kalla, R, Adams, A, Drummond, HE, Boyapati, R, Nimmo, ER, Wilson, DC, Satsangi, J, Heath, SC, Gut, M, Merkel, A, Bayes, M, Gut, IG, Keita, ÅV, Söderholm, JD, Hjortswang, H, Carstens, A, Bergemalm, D, Halfvarson, J, Andersson, E, Lindqvist, M, Repsilber, D, Pierik, M , Jonkers, D, Gomollón, F, D’Amato, M, Törkvist, L, Hjelm, F, Gullberg, M, Nordberg, N, Ocklind, A, Pettersson, E, Ekman, D, Sundell, M & IBD Character Consortium 2018, 'The prevalence and transcriptional activity of the mucosal microbiota of ulcerative colitis patients', Scientific Reports, vol. 8, no. 1, 17278. https://doi.org/10.1038/s41598-018-35243-4

TY - JOUR

T1 - The prevalence and transcriptional activity of the mucosal microbiota of ulcerative colitis patients

AU - Moen, Aina E.Fossum

AU - Lindstrøm, Jonas Christoffer

AU - Tannæs, Tone Møller

AU - Vatn, Simen

AU - Ricanek, Petr

AU - Vatn, Morten H.

AU - Jahnsen, Jørgen

AU - Frengen, Anna B.

AU - Dahl, Fredrik A.

AU - You, Panpan

AU - Sølvernes, Janne

AU - Ekeland, Gunn S.

AU - Detlie, Trond E.

AU - Olbjørn, Christine

AU - O’Leary, Kate R.

AU - Ventham, Nicholas T.

AU - Kennedy, Nicholas A.

AU - Kalla, Rahul

AU - Adams, Alex

AU - Drummond, Hazel E.

AU - Boyapati, Ray

AU - Nimmo, Elaine R.

AU - Wilson, David C.

AU - Satsangi, Jack

AU - Heath, Simon C.

AU - Gut, Marta

AU - Merkel, Angelika

AU - Bayes, Monica

AU - Gut, Ivo G.

AU - Keita, Åsa V.

AU - Söderholm, Johan D.

AU - Hjortswang, Henrik

AU - Carstens, Adam

AU - Bergemalm, Daniel

AU - Halfvarson, Jonas

AU - Andersson, Erik

AU - Lindqvist, Mårten

AU - Repsilber, Dirk

AU - Pierik, Marieke

AU - Jonkers, Daisy

AU - Gomollón, Fernando

AU - D’Amato, Mauro

AU - Törkvist, Leif

AU - Hjelm, Fredrik

AU - Gullberg, Mats

AU - Nordberg, Niklas

AU - Ocklind, Anette

AU - Pettersson, Erik

AU - Ekman, Daniel

AU - Sundell, Mikael

AU - IBD Character Consortium

N1 - Funding Information: The project was funded by the EU study IBD-Character (EU ref no 305676). We want to thank Engineer Sumana Kalyanasundaram, Institute of Informatics, University of Oslo, Norway for the bioinformatical help during the startup phase of the project. European IBD Character Consortium, FP7, Grant Agreement Number: 305676. Publisher Copyright: © 2018, The Author(s).

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Active microbes likely have larger impact on gut health status compared to inactive or dormant microbes. We investigate the composition of active and total mucosal microbiota of treatment-naïve ulcerative colitis (UC) patients to determine the microbial picture at the start-up phase of disease, using both a 16S rRNA transcript and gene amplicon sequencing. DNA and RNA were isolated from the same mucosal colonic biopsies. Our aim was to identify active microbial members of the microbiota in early stages of disease and reveal which members are present, but do not act as major players. We demonstrated differences in active and total microbiota of UC patients when comparing inflamed to non-inflamed tissue. Several taxa, among them the Proteobacteria phyla and families therein, revealed lower transcriptional activity despite a high presence. The Bifidobacteriaceae family of the Actinobacteria phylum showed lower abundance in the active microbiota, although no difference in presence was detected. The most abundant microbiota members of the inflamed tissue in UC patients were not the most active. Knowledge of active members of microbiota in UC patients could enhance our understanding of disease etiology. The active microbial community composition did not deviate from the total when comparing UC patients to non-IBD controls.

AB - Active microbes likely have larger impact on gut health status compared to inactive or dormant microbes. We investigate the composition of active and total mucosal microbiota of treatment-naïve ulcerative colitis (UC) patients to determine the microbial picture at the start-up phase of disease, using both a 16S rRNA transcript and gene amplicon sequencing. DNA and RNA were isolated from the same mucosal colonic biopsies. Our aim was to identify active microbial members of the microbiota in early stages of disease and reveal which members are present, but do not act as major players. We demonstrated differences in active and total microbiota of UC patients when comparing inflamed to non-inflamed tissue. Several taxa, among them the Proteobacteria phyla and families therein, revealed lower transcriptional activity despite a high presence. The Bifidobacteriaceae family of the Actinobacteria phylum showed lower abundance in the active microbiota, although no difference in presence was detected. The most abundant microbiota members of the inflamed tissue in UC patients were not the most active. Knowledge of active members of microbiota in UC patients could enhance our understanding of disease etiology. The active microbial community composition did not deviate from the total when comparing UC patients to non-IBD controls.

U2 - 10.1038/s41598-018-35243-4

DO - 10.1038/s41598-018-35243-4

M3 - Article

SN - 2045-2322

VL - 8

JO - Scientific Reports

JF - Scientific Reports

IS - 1

M1 - 17278

ER -

The prevalence and transcriptional activity of the mucosal microbiota of ulcerative colitis patients

Abstract

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