The mesoSPIM initiative: open-source light-sheet microscopes for imaging cleared tissue

Fabian F Voigt; Daniel Kirschenbaum; Evgenia Platonova; Stéphane Pagès; Robert A A Campbell; Rahel Kastli; Martina Schaettin; Ladan Egolf; Alexander van der Bourg; Philipp Bethge; Karen Haenraets; Noémie Frézel; Thomas Topilko; Paola Perin; Daniel Hillier; Sven Hildebrand; Anna Schueth; Alard Roebroeck; Botond Roska; Esther T Stoeckli; Roberto Pizzala; Nicolas Renier; Hanns Ulrich Zeilhofer; Theofanis Karayannis; Urs Ziegler; Laura Batti; Anthony Holtmaat; Christian Lüscher; Adriano Aguzzi; Fritjof Helmchen

doi:10.1038/s41592-019-0554-0

The mesoSPIM initiative: open-source light-sheet microscopes for imaging cleared tissue

Fabian F Voigt^*, Daniel Kirschenbaum, Evgenia Platonova, Stéphane Pagès, Robert A A Campbell, Rahel Kastli, Martina Schaettin, Ladan Egolf, Alexander van der Bourg, Philipp Bethge, Karen Haenraets, Noémie Frézel, Thomas Topilko, Paola Perin, Daniel Hillier, Sven Hildebrand, Anna Schueth, Alard Roebroeck, Botond Roska, Esther T StoeckliRoberto Pizzala, Nicolas Renier, Hanns Ulrich Zeilhofer, Theofanis Karayannis, Urs Ziegler, Laura Batti, Anthony Holtmaat, Christian Lüscher, Adriano Aguzzi, Fritjof Helmchen

^*Corresponding author for this work

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Abstract

Light-sheet microscopy is an ideal technique for imaging large cleared samples; however, the community is still lacking instruments capable of producing volumetric images of centimeter-sized cleared samples with near-isotropic resolution within minutes. Here, we introduce the mesoscale selective plane-illumination microscopy initiative, an open-hardware project for building and operating a light-sheet microscope that addresses these challenges and is compatible with any type of cleared or expanded sample ( www.mesospim.org ).

Original language	English
Pages (from-to)	1105-1108
Number of pages	4
Journal	Nature Methods
Volume	16
Issue number	11
Early online date	16 Sept 2019
DOIs	https://doi.org/10.1038/s41592-019-0554-0
Publication status	Published - Nov 2019

Keywords

ORGANS

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Voigt, F. F., Kirschenbaum, D., Platonova, E., Pagès, S., Campbell, R. A. A., Kastli, R., Schaettin, M., Egolf, L., van der Bourg, A., Bethge, P., Haenraets, K., Frézel, N., Topilko, T., Perin, P., Hillier, D., Hildebrand, S., Schueth, A., Roebroeck, A., Roska, B., ... Helmchen, F. (2019). The mesoSPIM initiative: open-source light-sheet microscopes for imaging cleared tissue. Nature Methods, 16(11), 1105-1108. https://doi.org/10.1038/s41592-019-0554-0

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title = "The mesoSPIM initiative: open-source light-sheet microscopes for imaging cleared tissue",

abstract = "Light-sheet microscopy is an ideal technique for imaging large cleared samples; however, the community is still lacking instruments capable of producing volumetric images of centimeter-sized cleared samples with near-isotropic resolution within minutes. Here, we introduce the mesoscale selective plane-illumination microscopy initiative, an open-hardware project for building and operating a light-sheet microscope that addresses these challenges and is compatible with any type of cleared or expanded sample ( www.mesospim.org ).",

keywords = "ORGANS",

author = "Voigt, {Fabian F} and Daniel Kirschenbaum and Evgenia Platonova and St{\'e}phane Pag{\`e}s and Campbell, {Robert A A} and Rahel Kastli and Martina Schaettin and Ladan Egolf and {van der Bourg}, Alexander and Philipp Bethge and Karen Haenraets and No{\'e}mie Fr{\'e}zel and Thomas Topilko and Paola Perin and Daniel Hillier and Sven Hildebrand and Anna Schueth and Alard Roebroeck and Botond Roska and Stoeckli, {Esther T} and Roberto Pizzala and Nicolas Renier and Zeilhofer, {Hanns Ulrich} and Theofanis Karayannis and Urs Ziegler and Laura Batti and Anthony Holtmaat and Christian L{\"u}scher and Adriano Aguzzi and Fritjof Helmchen",

note = "Funding Information: This work was supported by grants from the Swiss National Science Foundation (grant nos. 31003A-149858, 31003B-170269 to F.H.; no. 31003A_170037 to T.K.; nos. Funding Information: 31003A-153448, 31003A_173125, CRSII3_154453 and NCCR Synapsy no. 51NF40-158776 to A.H.), the European Research Council (ERC Advanced Grant BRAINCOMPATH, project no. 670757 to F.H.), ERC Starting Grants (InterWiring, project no. 679175 to T.K. and MULTICONNECT, project no. 639938 to A.R.), the Dutch science foundation (NWO VIDI Grant, project no. 14637 to A.R.), a PhD fellowship by the Swiss Foundation for Excellence in Biomedical Research (to R.K.), a gift from a private foundation with public interest through the International Foundation for Research in Paraplegia (to A.H. and S.P.), and a Distinguished Scientist Award of the Nomis Foundation (to A.A.). In addition, we would like to thank D. G{\"o}ckeritz-Dujmovic and S. Bichet for help with sample preparation and M. Wieckhorst for help with custom electronics. Publisher Copyright: {\textcopyright} 2019, The Author(s), under exclusive licence to Springer Nature America, Inc.",

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Voigt, FF, Kirschenbaum, D, Platonova, E, Pagès, S, Campbell, RAA, Kastli, R, Schaettin, M, Egolf, L, van der Bourg, A, Bethge, P, Haenraets, K, Frézel, N, Topilko, T, Perin, P, Hillier, D, Hildebrand, S , Schueth, A , Roebroeck, A, Roska, B, Stoeckli, ET, Pizzala, R, Renier, N, Zeilhofer, HU, Karayannis, T, Ziegler, U, Batti, L, Holtmaat, A, Lüscher, C, Aguzzi, A & Helmchen, F 2019, 'The mesoSPIM initiative: open-source light-sheet microscopes for imaging cleared tissue', Nature Methods, vol. 16, no. 11, pp. 1105-1108. https://doi.org/10.1038/s41592-019-0554-0

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T2 - open-source light-sheet microscopes for imaging cleared tissue

AU - Voigt, Fabian F

AU - Kirschenbaum, Daniel

AU - Platonova, Evgenia

AU - Pagès, Stéphane

AU - Campbell, Robert A A

AU - Kastli, Rahel

AU - Schaettin, Martina

AU - Egolf, Ladan

AU - van der Bourg, Alexander

AU - Bethge, Philipp

AU - Haenraets, Karen

AU - Frézel, Noémie

AU - Topilko, Thomas

AU - Perin, Paola

AU - Hillier, Daniel

AU - Hildebrand, Sven

AU - Schueth, Anna

AU - Roebroeck, Alard

AU - Roska, Botond

AU - Stoeckli, Esther T

AU - Pizzala, Roberto

AU - Renier, Nicolas

AU - Zeilhofer, Hanns Ulrich

AU - Karayannis, Theofanis

AU - Ziegler, Urs

AU - Batti, Laura

AU - Holtmaat, Anthony

AU - Lüscher, Christian

AU - Aguzzi, Adriano

AU - Helmchen, Fritjof

N1 - Funding Information: This work was supported by grants from the Swiss National Science Foundation (grant nos. 31003A-149858, 31003B-170269 to F.H.; no. 31003A_170037 to T.K.; nos. Funding Information: 31003A-153448, 31003A_173125, CRSII3_154453 and NCCR Synapsy no. 51NF40-158776 to A.H.), the European Research Council (ERC Advanced Grant BRAINCOMPATH, project no. 670757 to F.H.), ERC Starting Grants (InterWiring, project no. 679175 to T.K. and MULTICONNECT, project no. 639938 to A.R.), the Dutch science foundation (NWO VIDI Grant, project no. 14637 to A.R.), a PhD fellowship by the Swiss Foundation for Excellence in Biomedical Research (to R.K.), a gift from a private foundation with public interest through the International Foundation for Research in Paraplegia (to A.H. and S.P.), and a Distinguished Scientist Award of the Nomis Foundation (to A.A.). In addition, we would like to thank D. Göckeritz-Dujmovic and S. Bichet for help with sample preparation and M. Wieckhorst for help with custom electronics. Publisher Copyright: © 2019, The Author(s), under exclusive licence to Springer Nature America, Inc.

PY - 2019/11

Y1 - 2019/11

N2 - Light-sheet microscopy is an ideal technique for imaging large cleared samples; however, the community is still lacking instruments capable of producing volumetric images of centimeter-sized cleared samples with near-isotropic resolution within minutes. Here, we introduce the mesoscale selective plane-illumination microscopy initiative, an open-hardware project for building and operating a light-sheet microscope that addresses these challenges and is compatible with any type of cleared or expanded sample ( www.mesospim.org ).

AB - Light-sheet microscopy is an ideal technique for imaging large cleared samples; however, the community is still lacking instruments capable of producing volumetric images of centimeter-sized cleared samples with near-isotropic resolution within minutes. Here, we introduce the mesoscale selective plane-illumination microscopy initiative, an open-hardware project for building and operating a light-sheet microscope that addresses these challenges and is compatible with any type of cleared or expanded sample ( www.mesospim.org ).

KW - ORGANS

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DO - 10.1038/s41592-019-0554-0

M3 - Article

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SN - 1548-7091

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SP - 1105

EP - 1108

JO - Nature Methods

JF - Nature Methods

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