The mesoSPIM initiative: open-source light-sheet microscopes for imaging cleared tissue

Fabian F Voigt*, Daniel Kirschenbaum, Evgenia Platonova, Stéphane Pagès, Robert A A Campbell, Rahel Kastli, Martina Schaettin, Ladan Egolf, Alexander van der Bourg, Philipp Bethge, Karen Haenraets, Noémie Frézel, Thomas Topilko, Paola Perin, Daniel Hillier, Sven Hildebrand, Anna Schueth, Alard Roebroeck, Botond Roska, Esther T StoeckliRoberto Pizzala, Nicolas Renier, Hanns Ulrich Zeilhofer, Theofanis Karayannis, Urs Ziegler, Laura Batti, Anthony Holtmaat, Christian Lüscher, Adriano Aguzzi, Fritjof Helmchen

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Light-sheet microscopy is an ideal technique for imaging large cleared samples; however, the community is still lacking instruments capable of producing volumetric images of centimeter-sized cleared samples with near-isotropic resolution within minutes. Here, we introduce the mesoscale selective plane-illumination microscopy initiative, an open-hardware project for building and operating a light-sheet microscope that addresses these challenges and is compatible with any type of cleared or expanded sample ( ).

Original languageEnglish
Pages (from-to)1105-1108
Number of pages4
JournalNature Methods
Issue number11
Early online date16 Sep 2019
Publication statusPublished - Nov 2019



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