The gut resistome is highly dynamic during the first months of life

Christian J. H. von Wintersdorff; Petra F. G. Wolffs; Paul H. M. Savelkoul; Rianne R. R. Nijsen; Susanne Lau; Kerstin Gerhold; Eckard Hamelmann; John Penders

doi:10.2217/fmb.15.154

The gut resistome is highly dynamic during the first months of life

Christian J. H. von Wintersdorff, Petra F. G. Wolffs, Paul H. M. Savelkoul, Rianne R. R. Nijsen, Susanne Lau, Kerstin Gerhold, Eckard Hamelmann, John Penders^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Aim: We investigated the longitudinal development of several antibiotic resistance genes (ARGs) of the infant gut resistome during the first months after birth. Materials & methods: Fecal samples from 120 infants collected at the ages of 5, 13 and 31 weeks were analyzed and subjected to qPCR for the detection of several ARGs. Results: The prevalence of ARGs significantly increased for ermB, tetM and tetQ, while it decreased for aac(6')-aph(2'). Birth mode and breastfeeding significantly affected tetQ prevalence. Correlations to bacterial taxa suggest that fluctuations in some ARGs are (partly) attributed to shifts in bacteroides colonization rates. Conclusion: Acquisition of ARGs in the gut microbiota occurs shortly after birth and resistome composition fluctuates over the course of several months, reflecting changes in microbial community structure.

Original language	English
Pages (from-to)	501-510
Number of pages	10
Journal	Future Microbiology
Volume	11
Issue number	4
DOIs	https://doi.org/10.2217/fmb.15.154
Publication status	Published - 2016

Keywords

antibiotic resistance gene
bacteroides
birth mode
breastfeeding
gut microbiota
infant
metagenome
microbiome
pediatric
resistomes

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10.2217/fmb.15.154

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title = "The gut resistome is highly dynamic during the first months of life",

abstract = "Aim: We investigated the longitudinal development of several antibiotic resistance genes (ARGs) of the infant gut resistome during the first months after birth. Materials & methods: Fecal samples from 120 infants collected at the ages of 5, 13 and 31 weeks were analyzed and subjected to qPCR for the detection of several ARGs. Results: The prevalence of ARGs significantly increased for ermB, tetM and tetQ, while it decreased for aac(6')-aph(2'). Birth mode and breastfeeding significantly affected tetQ prevalence. Correlations to bacterial taxa suggest that fluctuations in some ARGs are (partly) attributed to shifts in bacteroides colonization rates. Conclusion: Acquisition of ARGs in the gut microbiota occurs shortly after birth and resistome composition fluctuates over the course of several months, reflecting changes in microbial community structure.",

keywords = "antibiotic resistance gene, bacteroides, birth mode, breastfeeding, gut microbiota, infant, metagenome, microbiome, pediatric, resistomes",

author = "{von Wintersdorff}, {Christian J. H.} and Wolffs, {Petra F. G.} and Savelkoul, {Paul H. M.} and Nijsen, {Rianne R. R.} and Susanne Lau and Kerstin Gerhold and Eckard Hamelmann and John Penders",

year = "2016",

doi = "10.2217/fmb.15.154",

language = "English",

volume = "11",

pages = "501--510",

journal = "Future Microbiology",

issn = "1746-0913",

publisher = "Routledge/Taylor & Francis Group",

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TY - JOUR

T1 - The gut resistome is highly dynamic during the first months of life

AU - von Wintersdorff, Christian J. H.

AU - Wolffs, Petra F. G.

AU - Savelkoul, Paul H. M.

AU - Nijsen, Rianne R. R.

AU - Lau, Susanne

AU - Gerhold, Kerstin

AU - Hamelmann, Eckard

AU - Penders, John

PY - 2016

Y1 - 2016

N2 - Aim: We investigated the longitudinal development of several antibiotic resistance genes (ARGs) of the infant gut resistome during the first months after birth. Materials & methods: Fecal samples from 120 infants collected at the ages of 5, 13 and 31 weeks were analyzed and subjected to qPCR for the detection of several ARGs. Results: The prevalence of ARGs significantly increased for ermB, tetM and tetQ, while it decreased for aac(6')-aph(2'). Birth mode and breastfeeding significantly affected tetQ prevalence. Correlations to bacterial taxa suggest that fluctuations in some ARGs are (partly) attributed to shifts in bacteroides colonization rates. Conclusion: Acquisition of ARGs in the gut microbiota occurs shortly after birth and resistome composition fluctuates over the course of several months, reflecting changes in microbial community structure.

AB - Aim: We investigated the longitudinal development of several antibiotic resistance genes (ARGs) of the infant gut resistome during the first months after birth. Materials & methods: Fecal samples from 120 infants collected at the ages of 5, 13 and 31 weeks were analyzed and subjected to qPCR for the detection of several ARGs. Results: The prevalence of ARGs significantly increased for ermB, tetM and tetQ, while it decreased for aac(6')-aph(2'). Birth mode and breastfeeding significantly affected tetQ prevalence. Correlations to bacterial taxa suggest that fluctuations in some ARGs are (partly) attributed to shifts in bacteroides colonization rates. Conclusion: Acquisition of ARGs in the gut microbiota occurs shortly after birth and resistome composition fluctuates over the course of several months, reflecting changes in microbial community structure.

KW - antibiotic resistance gene

KW - bacteroides

KW - birth mode

KW - breastfeeding

KW - gut microbiota

KW - infant

KW - metagenome

KW - microbiome

KW - pediatric

KW - resistomes

U2 - 10.2217/fmb.15.154

DO - 10.2217/fmb.15.154

M3 - Article

C2 - 27064174

SN - 1746-0913

VL - 11

SP - 501

EP - 510

JO - Future Microbiology

JF - Future Microbiology

IS - 4

ER -

The gut resistome is highly dynamic during the first months of life

Abstract

Keywords

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