The Gut Microbiota from Lean and Obese Subjects Contribute Differently to the Fermentation of Arabinogalactan and Inulin

Marisol Aguirre Morales, C. Bussolo de Souza, Koen Venema

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Background

An aberrant metabolic activity or a compositional alteration of the gut microbiota has been proposed as a factor that makes us more prone to disease. Therefore, we explored the effect of two dietary fibers (arabinogalactan and inulin) on the microbiota from lean and obese subjects during 72 h in vitro fermentation experiments using the validated TNO dynamic in vitro model of the proximal colon: TIM-2. Metabolically, arabinogalactan fermentation showed a higher production of propionate when compared to n-butyrate in the obese microbiota fermentations. In general, lean microbiota produced more n-butyrate from the fermentation of both substrates when compared to the obese microbiota. Furthermore, the obese microbiota extracted more energy from the fermentation of both fibers.

Results

Compositionally, bacteria belonging to Gemmiger, Dorea, Roseburia, Alistipes, Lactobacillus and Bifidobacterium genera were found to be highly abundant or stimulated by the prebiotics in the lean microbiota suggesting a potential role in leanness. Furthermore, a significant correlation between known butyrogenic strains including B. adolescentis, an unclassified Bifidobacterium and F. prausnitzii with this metabolite in the fermentation of inulin in both microbiotas was found.

Conclusions

Although supplementary in vivo studies are needed, the current study provides more evidence for the consumption of specific ingredients with the aim of modulating the gut microbiota in the context of obesity.

Original languageEnglish
Article numbere0159236
Number of pages18
JournalPLOS ONE
Volume11
Issue number7
DOIs
Publication statusPublished - 13 Jul 2016

Keywords

  • IN-VITRO FERMENTATION
  • CHAIN FATTY-ACIDS
  • OLIGOFRUCTOSE-ENRICHED INULIN
  • NONSTARCH POLYSACCHARIDES
  • FECAL MICROBIOTA
  • PROPIONIC-ACID
  • DIETARY FIBER
  • BACTEROIDES-THETAIOTAOMICRON
  • RESISTANT STARCH
  • LARGE-INTESTINE

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