Abstract
Background The enteric nervous system (ENS) is an extensive neural network embedded in the wall of the gastrointestinal tract that regulates digestive function and gastrointestinal homeostasis. The ENS consists of two main cell types; enteric neurons and enteric glial cells. In vitro techniques allow simplified investigation of ENS function, and different culture methods have been developed over the years helping to understand the role of ENS cells in health and disease. Purpose This review focuses on summarizing and comparing available culture protocols for the generation of primary ENS cells from adult mice, including dissection of intestinal segments, enzymatic digestions, surface coatings, and culture media. In addition, the potential of human ENS cultures is also discussed.
Original language | English |
---|---|
Article number | 14215 |
Number of pages | 16 |
Journal | Neurogastroenterology and Motility |
Volume | 34 |
Issue number | 2 |
Early online date | 8 Jul 2021 |
DOIs | |
Publication status | Published - Feb 2022 |
Keywords
- adult mouse
- enteric glial cells
- enteric neurons
- primary ENS culture
- protocol
- PLURIPOTENT STEM-CELLS
- NEURAL CREST CELLS
- GUINEA-PIG
- MYENTERIC PLEXUS
- GLIAL-CELLS
- EXTRACELLULAR-MATRIX
- MAST-CELL
- NEURONS
- MOUSE
- DIFFERENTIATION