The flavanol (-)-epicatechin and its metabolites protect against oxidative stress in primary endothelial cells via a direct antioxidant effect.

E.J.B. Ruijters, A.R. Weseler, C. Kicken, G.R.M.M. Haenen, A. Bast

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

Accumulating evidence suggests that foods rich in flavanols decrease the developing cardiovascular diseases. Attenuation of oxidative stress was to contribute to the cardiovascular benefit of flavanols. Up to now it unclear whether flavanol metabolites can also protect cells from stress. The aim of the present study was to determine the potential of several glucuronidated, methylated and sulfated metabolites of (-)- (EC) and (+)-catechin (Cat) to the protection of human vascular (HUVECs) against oxidative stress. The relative potency of the tested to scavenge superoxide anion radicals showed that a free catechol moiety molecule is important for the direct antioxidant activity. EC and Cat 10microM) were potent radical scavengers and provided protection against intracellular oxidative stress induced by hydrogen peroxide. Although metabolites provided less intracellular protection compared to EC and tested methylated and glucuronidated metabolites reduced oxidative significantly in HUVECs. Our results indicate that the metabolites have relevant contribution in the intracellular protection of EC and Cat oxidative stress. Also, the direct antioxidant activity plays an in this protection.
Original languageEnglish
Pages (from-to)147-153
Number of pages7
JournalEuropean Journal of Pharmacology
Volume715
Issue number1-3
DOIs
Publication statusPublished - 5 Sep 2013

Keywords

  • DCFH assay
  • ROS
  • HUVEC
  • Antioxidant
  • Nrf2
  • CHOCOLATE CONSUMPTION
  • HYDROGEN-PEROXIDE
  • NADPH OXIDASE
  • IN-VIVO
  • CARDIOVASCULAR-DISEASE
  • BETA-GLUCURONIDASE
  • REACTIVE OXYGEN
  • HUMAN PLASMA
  • RICH COCOA
  • EPICATECHIN

Cite this

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title = "The flavanol (-)-epicatechin and its metabolites protect against oxidative stress in primary endothelial cells via a direct antioxidant effect.",
abstract = "Accumulating evidence suggests that foods rich in flavanols decrease the developing cardiovascular diseases. Attenuation of oxidative stress was to contribute to the cardiovascular benefit of flavanols. Up to now it unclear whether flavanol metabolites can also protect cells from stress. The aim of the present study was to determine the potential of several glucuronidated, methylated and sulfated metabolites of (-)- (EC) and (+)-catechin (Cat) to the protection of human vascular (HUVECs) against oxidative stress. The relative potency of the tested to scavenge superoxide anion radicals showed that a free catechol moiety molecule is important for the direct antioxidant activity. EC and Cat 10microM) were potent radical scavengers and provided protection against intracellular oxidative stress induced by hydrogen peroxide. Although metabolites provided less intracellular protection compared to EC and tested methylated and glucuronidated metabolites reduced oxidative significantly in HUVECs. Our results indicate that the metabolites have relevant contribution in the intracellular protection of EC and Cat oxidative stress. Also, the direct antioxidant activity plays an in this protection.",
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author = "E.J.B. Ruijters and A.R. Weseler and C. Kicken and G.R.M.M. Haenen and A. Bast",
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The flavanol (-)-epicatechin and its metabolites protect against oxidative stress in primary endothelial cells via a direct antioxidant effect. / Ruijters, E.J.B.; Weseler, A.R.; Kicken, C.; Haenen, G.R.M.M.; Bast, A.

In: European Journal of Pharmacology, Vol. 715, No. 1-3, 05.09.2013, p. 147-153.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - The flavanol (-)-epicatechin and its metabolites protect against oxidative stress in primary endothelial cells via a direct antioxidant effect.

AU - Ruijters, E.J.B.

AU - Weseler, A.R.

AU - Kicken, C.

AU - Haenen, G.R.M.M.

AU - Bast, A.

PY - 2013/9/5

Y1 - 2013/9/5

N2 - Accumulating evidence suggests that foods rich in flavanols decrease the developing cardiovascular diseases. Attenuation of oxidative stress was to contribute to the cardiovascular benefit of flavanols. Up to now it unclear whether flavanol metabolites can also protect cells from stress. The aim of the present study was to determine the potential of several glucuronidated, methylated and sulfated metabolites of (-)- (EC) and (+)-catechin (Cat) to the protection of human vascular (HUVECs) against oxidative stress. The relative potency of the tested to scavenge superoxide anion radicals showed that a free catechol moiety molecule is important for the direct antioxidant activity. EC and Cat 10microM) were potent radical scavengers and provided protection against intracellular oxidative stress induced by hydrogen peroxide. Although metabolites provided less intracellular protection compared to EC and tested methylated and glucuronidated metabolites reduced oxidative significantly in HUVECs. Our results indicate that the metabolites have relevant contribution in the intracellular protection of EC and Cat oxidative stress. Also, the direct antioxidant activity plays an in this protection.

AB - Accumulating evidence suggests that foods rich in flavanols decrease the developing cardiovascular diseases. Attenuation of oxidative stress was to contribute to the cardiovascular benefit of flavanols. Up to now it unclear whether flavanol metabolites can also protect cells from stress. The aim of the present study was to determine the potential of several glucuronidated, methylated and sulfated metabolites of (-)- (EC) and (+)-catechin (Cat) to the protection of human vascular (HUVECs) against oxidative stress. The relative potency of the tested to scavenge superoxide anion radicals showed that a free catechol moiety molecule is important for the direct antioxidant activity. EC and Cat 10microM) were potent radical scavengers and provided protection against intracellular oxidative stress induced by hydrogen peroxide. Although metabolites provided less intracellular protection compared to EC and tested methylated and glucuronidated metabolites reduced oxidative significantly in HUVECs. Our results indicate that the metabolites have relevant contribution in the intracellular protection of EC and Cat oxidative stress. Also, the direct antioxidant activity plays an in this protection.

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KW - IN-VIVO

KW - CARDIOVASCULAR-DISEASE

KW - BETA-GLUCURONIDASE

KW - REACTIVE OXYGEN

KW - HUMAN PLASMA

KW - RICH COCOA

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DO - 10.1016/j.ejphar.2013.05.029

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