@article{a5870cda96fb4c5498c40e3213d020dd,
title = "The fecal and mucosal microbiome in acute appendicitis patients: an observational study",
abstract = "Aim: We aimed to study the mucosal microbiota of the appendix in a prospective appendicitis cohort and to compare the fecal microbiota of patients and controls. We hypothesized that the microbiota may be associated with susceptibility to appendicitis. Patients & methods: The fecal microbiota of 99 patients and 106 controls were characterized using 16S-23S intergenic spacer profiling. Richness, diversity and community structure were compared. The appendiceal microbiota from 90 patients was analyzed according to the severity of appendicitis. Results: Overall fecal microbial richness and diversity were similar in patients and controls, yet richness and diversity within the group of Firmicutes, Actinobacteria, Fusobacteria and Verrucomicrobia phyla were lower in patients. Discriminant analyses could correctly classify patients and controls with fair accuracy. No differences were found according to severity in appendiceal or fecal microbiota. Conclusion: This study demonstrates differences in the composition of intestinal microbiota of appendicitis patients and healthy individuals.",
keywords = "APPENDECTOMY, BURDEN, DISEASE, GUT MICROBIOME, acute appendicitis, adult population, dysbiosis, intestinal microbiota, microbiome, prospective cohort study, CHILDREN",
author = "Toon Peeters and John Penders and Smeekens, {Sanne P.} and Gianluca Galazzo and Bert Houben and Netea, {Mihai G.} and Savelkoul, {Paul H. M.} and Gyssens, {Inge C.}",
note = "Funding Information: This study is a part of the Limburg Clinical Research Program, UHasselt-ZOL-Jessa, supported by the foundation Limburg Sterk Merk, province of Limburg, Flemish government, Hasselt University, Ziekenhuis Oost-Limburg and Jessa Hospital. The study was supported by ESCMID Study Group on Molecular Diagnostics research (grant number #9334). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. No writing assistance was utilized in the production of this manuscript. Funding Information: Financial & competing interests disclosures This study is a part of the Limburg Clinical Research Program, UHasselt-ZOL-Jessa, supported by the foundation Limburg Sterk Merk, province of Limburg, Flemish government, Hasselt University, Ziekenhuis Oost-Limburg and Jessa Hospital. The study was supported by ESCMID Study Group on Molecular Diagnostics research (grant number #9334). The authors have no other relevant affiliations or financial involvement with any organization or entity with a financial interest in or financial conflict with the subject matter or materials discussed in the manuscript apart from those disclosed. Publisher Copyright: {\textcopyright} 2019 2019 Future Medicine Ltd.",
year = "2019",
month = jan,
doi = "10.2217/fmb-2018-0203",
language = "English",
volume = "14",
pages = "111--127",
journal = "Future Microbiology",
issn = "1746-0913",
publisher = "Future Medicine Ltd.",
number = "2",
}