The effects of polyphenol supplementation on adipose tissue morphology and gene expression in overweight and obese humans

Jasper Most; Ines Warnke; Mark V. Boekschoten; Johan W. E. Jocken; Philip de Groot; Angelika Friedel; Igor Bendik; Gijs H. Goossens; Ellen E. Blaak

doi:10.1080/21623945.2018.1469942

The effects of polyphenol supplementation on adipose tissue morphology and gene expression in overweight and obese humans

Jasper Most
, Ines Warnke
, Mark V. Boekschoten
, Johan W. E. Jocken
, Philip de Groot
, Angelika Friedel
, Igor Bendik
, Gijs H. Goossens
, Ellen E. Blaak^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

Dietary polyphenols have beneficial effects on adipose tissue mass and function in rodents, but human studies are scarce. In a randomized, placebo-controlled study, 25 (10 women) overweight and obese humans received a combination of the polyphenols epigallocatechin-gallate and resveratrol (282mg/d, 80mg/d, respectively, EGCG+RES, n = 11) or placebo (PLA, n = 14) supplementation for 12weeks. Abdominal subcutaneous adipose tissue (SAT) biopsies were collected for assessment of adipocyte morphology and micro-array analysis. EGCG+RES had no effects on adipocyte size and distribution compared with PLA. However, we identified pathways contributing to adipogenesis, cell cycle and apoptosis were significantly downregulated by EGCG+RES versus PLA. Furthermore, EGCG+RES significantly decreased expression of pathways related to energy metabolism, oxidative stress, inflammation, and immune defense as compared with PLA. In conclusion, the SAT gene expression profile indicates a reduced cell turnover after 12-week EGCG+RES in overweight-obese subjects. It remains to be elucidated whether these alterations translate into long-term metabolic effects.

Original language	English
Pages (from-to)	190-196
Number of pages	7
Journal	Adipocyte
Volume	7
Issue number	3
DOIs	https://doi.org/10.1080/21623945.2018.1469942
Publication status	Published - 1 Jan 2018

Keywords

Adipose tissue
EGCG
Morphology
Obesity
Resveratrol
Transcriptomics
RESVERATROL SUPPLEMENTATION
INSULIN-RESISTANCE
METABOLIC DISEASE
TURNOVER
EPIGALLOCATECHIN-3-GALLATE
MECHANISMS
WEIGHT
WOMEN
TRIAL
MICE

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title = "The effects of polyphenol supplementation on adipose tissue morphology and gene expression in overweight and obese humans",

abstract = "Dietary polyphenols have beneficial effects on adipose tissue mass and function in rodents, but human studies are scarce. In a randomized, placebo-controlled study, 25 (10 women) overweight and obese humans received a combination of the polyphenols epigallocatechin-gallate and resveratrol (282mg/d, 80mg/d, respectively, EGCG+RES, n = 11) or placebo (PLA, n = 14) supplementation for 12weeks. Abdominal subcutaneous adipose tissue (SAT) biopsies were collected for assessment of adipocyte morphology and micro-array analysis. EGCG+RES had no effects on adipocyte size and distribution compared with PLA. However, we identified pathways contributing to adipogenesis, cell cycle and apoptosis were significantly downregulated by EGCG+RES versus PLA. Furthermore, EGCG+RES significantly decreased expression of pathways related to energy metabolism, oxidative stress, inflammation, and immune defense as compared with PLA. In conclusion, the SAT gene expression profile indicates a reduced cell turnover after 12-week EGCG+RES in overweight-obese subjects. It remains to be elucidated whether these alterations translate into long-term metabolic effects.",

keywords = "Adipose tissue, EGCG, Morphology, Obesity, Resveratrol, Transcriptomics, RESVERATROL SUPPLEMENTATION, INSULIN-RESISTANCE, METABOLIC DISEASE, TURNOVER, EPIGALLOCATECHIN-3-GALLATE, MECHANISMS, WEIGHT, WOMEN, TRIAL, MICE",

author = "Jasper Most and Ines Warnke and Boekschoten, \{Mark V.\} and Jocken, \{Johan W. E.\} and \{de Groot\}, Philip and Angelika Friedel and Igor Bendik and Goossens, \{Gijs H.\} and Blaak, \{Ellen E.\}",

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doi = "10.1080/21623945.2018.1469942",

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T1 - The effects of polyphenol supplementation on adipose tissue morphology and gene expression in overweight and obese humans

AU - Most, Jasper

AU - Warnke, Ines

AU - Boekschoten, Mark V.

AU - Jocken, Johan W. E.

AU - de Groot, Philip

AU - Friedel, Angelika

AU - Bendik, Igor

AU - Goossens, Gijs H.

AU - Blaak, Ellen E.

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N2 - Dietary polyphenols have beneficial effects on adipose tissue mass and function in rodents, but human studies are scarce. In a randomized, placebo-controlled study, 25 (10 women) overweight and obese humans received a combination of the polyphenols epigallocatechin-gallate and resveratrol (282mg/d, 80mg/d, respectively, EGCG+RES, n = 11) or placebo (PLA, n = 14) supplementation for 12weeks. Abdominal subcutaneous adipose tissue (SAT) biopsies were collected for assessment of adipocyte morphology and micro-array analysis. EGCG+RES had no effects on adipocyte size and distribution compared with PLA. However, we identified pathways contributing to adipogenesis, cell cycle and apoptosis were significantly downregulated by EGCG+RES versus PLA. Furthermore, EGCG+RES significantly decreased expression of pathways related to energy metabolism, oxidative stress, inflammation, and immune defense as compared with PLA. In conclusion, the SAT gene expression profile indicates a reduced cell turnover after 12-week EGCG+RES in overweight-obese subjects. It remains to be elucidated whether these alterations translate into long-term metabolic effects.

AB - Dietary polyphenols have beneficial effects on adipose tissue mass and function in rodents, but human studies are scarce. In a randomized, placebo-controlled study, 25 (10 women) overweight and obese humans received a combination of the polyphenols epigallocatechin-gallate and resveratrol (282mg/d, 80mg/d, respectively, EGCG+RES, n = 11) or placebo (PLA, n = 14) supplementation for 12weeks. Abdominal subcutaneous adipose tissue (SAT) biopsies were collected for assessment of adipocyte morphology and micro-array analysis. EGCG+RES had no effects on adipocyte size and distribution compared with PLA. However, we identified pathways contributing to adipogenesis, cell cycle and apoptosis were significantly downregulated by EGCG+RES versus PLA. Furthermore, EGCG+RES significantly decreased expression of pathways related to energy metabolism, oxidative stress, inflammation, and immune defense as compared with PLA. In conclusion, the SAT gene expression profile indicates a reduced cell turnover after 12-week EGCG+RES in overweight-obese subjects. It remains to be elucidated whether these alterations translate into long-term metabolic effects.

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KW - METABOLIC DISEASE

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KW - MECHANISMS

KW - WEIGHT

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The effects of polyphenol supplementation on adipose tissue morphology and gene expression in overweight and obese humans

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Keywords

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