TY - JOUR
T1 - The effect of UCP3 overexpression on mitochondrial ROS production in skeletal muscle of young versus aged mice
AU - Nabben, M.
AU - Hoeks, J.
AU - Briede, J.J.
AU - Glatz, J.F.
AU - Kornips, E.
AU - Hesselink, M.K.
AU - Schrauwen, P.
PY - 2008/1/1
Y1 - 2008/1/1
N2 - Uncoupling protein 3 (UCP3) is suggested to protect mitochondria against aging and lipid-induced damage, possibly via modulation of reactive oxygen species (ROS) production. Here we show that mice overexpressing UCP3 (UCP3Tg) have a blunted age-induced increase in ROS production, assessed by electron spin resonance spectroscopy, but only after addition of 4-hydroxynonenal (4-HNE). Mitochondrial function, assessed by respirometry, on glycolytic substrate was lower in UCP3Tg mice compared to wild types, whereas this tended to be higher on fatty acids. State 4o respiration was higher in UCP3Tg animals. To conclude, UCP3 overexpression leads to increased state 4o respiration and, in presence of 4-HNE, blunts the age-induced increase in ROS production.
AB - Uncoupling protein 3 (UCP3) is suggested to protect mitochondria against aging and lipid-induced damage, possibly via modulation of reactive oxygen species (ROS) production. Here we show that mice overexpressing UCP3 (UCP3Tg) have a blunted age-induced increase in ROS production, assessed by electron spin resonance spectroscopy, but only after addition of 4-hydroxynonenal (4-HNE). Mitochondrial function, assessed by respirometry, on glycolytic substrate was lower in UCP3Tg mice compared to wild types, whereas this tended to be higher on fatty acids. State 4o respiration was higher in UCP3Tg animals. To conclude, UCP3 overexpression leads to increased state 4o respiration and, in presence of 4-HNE, blunts the age-induced increase in ROS production.
U2 - 10.1016/j.febslet.2008.11.016
DO - 10.1016/j.febslet.2008.11.016
M3 - Article
C2 - 19041310
SN - 0014-5793
VL - 582
SP - 4147
EP - 4152
JO - Febs Letters
JF - Febs Letters
IS - 30
ER -