The effect of trace amounts of tissue factor on thrombin generation in platelet rich plasma, its inhibition by heparin

S. Beguin, T. Lindhout, H.C. Hemker

    Research output: Contribution to journalArticleAcademicpeer-review

    76 Downloads (Pure)


    Amounts of human brain thromboplastin that do not stimulate thrombin generation in platelet poor plasma, were shown to advance by about 4 min an explosive formation of thrombin that occurs after recalcification in the presence of blood platelets. This synergistic effect is inhibited by the specific thrombin inhibitor hirudin and mimicked by adding low concentrations (< 5 nM) of thrombin to platelet rich plasma. It is our conclusion that, small amounts of thrombin, generated under the influence of thromboplastin induced procoagulant activity in the blood platelets. This activity is most likely mainly due to procoagulant phospholipids. Heparin inhibits this effect and retards the explosive thrombin formation. It does not, however, diminish the peak amount of thrombin eventually formed, because heparin neutralizing material released from the activated platelets quenches the heparin effect.
    Original languageEnglish
    Pages (from-to)25-29
    Number of pages5
    JournalThrombosis and Haemostasis
    Issue number1
    Publication statusPublished - 1989

    Cite this