The Effect of Sulforaphane on Glyoxalase I Expression and Activity in Peripheral Blood Mononuclear Cells

Michela Alfarano, Donato Pastore, Vincenzo Fogliano, Casper G. Schalkwijk, Teresa Oliviero*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Web of Science)

Abstract

Studies demonstrate that the potential health-beneficial effect of sulforaphane (SR), a compound formed in broccoli, is the result of a number of mechanisms including upregulation of phase two detoxification enzymes. Recent studies suggest that SR increases expression/activity of glyoxalase 1 (Glo1), an enzyme involved in the degradation of methylglyoxal, is major precursor of advanced glycation end products. Those compounds are associated with diabetes complications and other age-related diseases. In this study, the effect of SR on the expression/activity of Glo1 in peripheral blood mononuclear cells (PBMCs) from 8 healthy volunteers was investigated. PBMCs were isolated and incubated with SR (2.5 mu M-concentration achievable by consuming a broccoli portion) for 24 h and 48 h. Glo1 activity/expression, reduced glutathione (GSH), and glutathione-S-transferase gene expression were measured. Glo1 activity was not affected while after 48 h a slight but significant increase of its gene expression (1.03-fold) was observed. GSTP1 expression slightly increased after 24 h incubation (1.08-fold) while the expressions of isoform GSTT2 and GSTM2 were below the limit of detection. GSH sharply decreased, suggesting the formation of GSH-SR adducts that may have an impact SR availability. Those results suggest that a regular exposure to SR by broccoli consumption or SR supplements may enhance Glo1.

Original languageEnglish
Article number1773
Number of pages11
JournalNutrients
Volume10
Issue number11
DOIs
Publication statusPublished - Nov 2018

Keywords

  • glyoxalase 1
  • sulforaphane
  • peripheral blood mononuclear cells
  • glutathione
  • glutathione-S-transferase
  • GLYCATION END-PRODUCTS
  • ISOTHIOCYANATES
  • SYSTEM
  • INDUCTION
  • CHEMOPREVENTION
  • METHYLGLYOXAL
  • MECHANISMS
  • DEFENSE

Cite this