The effect of high-fat feeding on intramuscular lipid and lipid peroxidation levels in UCP3-ablated mice

J. Hoeks; M.K. Hesselink; W. Sluiter; G. Schaart; J. Willems; A. Morrisson; J.C. Clapham; W.H. Saris; P. Schrauwen

doi:10.1016/j.febslet.2006.01.059

The effect of high-fat feeding on intramuscular lipid and lipid peroxidation levels in UCP3-ablated mice

J. Hoeks^*
, M.K. Hesselink
, W. Sluiter
, G. Schaart
, J. Willems
, A. Morrisson
, J.C. Clapham
, W.H. Saris
, P. Schrauwen

^*Corresponding author for this work

NUTRIM - Institute of Nutrition and Translational Research in Metabolism

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Uncoupling protein-3 (UCP3) has been suggested to protect against lipid-induced oxidative damage. Therefore, we studied intramuscular lipid peroxide levels and high-fat diet induced alterations in muscle lipid metabolism of UCP3-ablated mice. UCP3(-/-) mice showed approximately 3-fold higher levels of intramuscular lipid peroxides upon standard chow feeding, compared to wild-type littermates. Remarkably, this difference was no longer apparent on the high-fat diet. However, upon high-fat feeding, intramuscular triacylglycerol levels were approximately 50% lower in UCP3(-/-) mice, in comparison to UCP3(+/+) animals. Succinate dehydrogenase activity, and total protein content of the muscle fatty acid transporter FAT/CD36 were however similar between UCP3(-/-) and UCP3(+/+) mice.

Original language	English
Pages (from-to)	1371-1375
Journal	Febs Letters
Volume	580
Issue number	5
DOIs	https://doi.org/10.1016/j.febslet.2006.01.059
Publication status	Published - 1 Jan 2006

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title = "The effect of high-fat feeding on intramuscular lipid and lipid peroxidation levels in UCP3-ablated mice",

abstract = "Uncoupling protein-3 (UCP3) has been suggested to protect against lipid-induced oxidative damage. Therefore, we studied intramuscular lipid peroxide levels and high-fat diet induced alterations in muscle lipid metabolism of UCP3-ablated mice. UCP3(-/-) mice showed approximately 3-fold higher levels of intramuscular lipid peroxides upon standard chow feeding, compared to wild-type littermates. Remarkably, this difference was no longer apparent on the high-fat diet. However, upon high-fat feeding, intramuscular triacylglycerol levels were approximately 50\% lower in UCP3(-/-) mice, in comparison to UCP3(+/+) animals. Succinate dehydrogenase activity, and total protein content of the muscle fatty acid transporter FAT/CD36 were however similar between UCP3(-/-) and UCP3(+/+) mice.",

author = "J. Hoeks and M.K. Hesselink and W. Sluiter and G. Schaart and J. Willems and A. Morrisson and J.C. Clapham and W.H. Saris and P. Schrauwen",

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T1 - The effect of high-fat feeding on intramuscular lipid and lipid peroxidation levels in UCP3-ablated mice

AU - Hoeks, J.

AU - Hesselink, M.K.

AU - Sluiter, W.

AU - Schaart, G.

AU - Willems, J.

AU - Morrisson, A.

AU - Clapham, J.C.

AU - Saris, W.H.

AU - Schrauwen, P.

PY - 2006/1/1

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N2 - Uncoupling protein-3 (UCP3) has been suggested to protect against lipid-induced oxidative damage. Therefore, we studied intramuscular lipid peroxide levels and high-fat diet induced alterations in muscle lipid metabolism of UCP3-ablated mice. UCP3(-/-) mice showed approximately 3-fold higher levels of intramuscular lipid peroxides upon standard chow feeding, compared to wild-type littermates. Remarkably, this difference was no longer apparent on the high-fat diet. However, upon high-fat feeding, intramuscular triacylglycerol levels were approximately 50% lower in UCP3(-/-) mice, in comparison to UCP3(+/+) animals. Succinate dehydrogenase activity, and total protein content of the muscle fatty acid transporter FAT/CD36 were however similar between UCP3(-/-) and UCP3(+/+) mice.

AB - Uncoupling protein-3 (UCP3) has been suggested to protect against lipid-induced oxidative damage. Therefore, we studied intramuscular lipid peroxide levels and high-fat diet induced alterations in muscle lipid metabolism of UCP3-ablated mice. UCP3(-/-) mice showed approximately 3-fold higher levels of intramuscular lipid peroxides upon standard chow feeding, compared to wild-type littermates. Remarkably, this difference was no longer apparent on the high-fat diet. However, upon high-fat feeding, intramuscular triacylglycerol levels were approximately 50% lower in UCP3(-/-) mice, in comparison to UCP3(+/+) animals. Succinate dehydrogenase activity, and total protein content of the muscle fatty acid transporter FAT/CD36 were however similar between UCP3(-/-) and UCP3(+/+) mice.

U2 - 10.1016/j.febslet.2006.01.059

DO - 10.1016/j.febslet.2006.01.059

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EP - 1375

JO - Febs Letters

JF - Febs Letters

IS - 5

ER -

The effect of high-fat feeding on intramuscular lipid and lipid peroxidation levels in UCP3-ablated mice

Abstract

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