Uncoupling protein-3 (UCP3) has been suggested to protect against lipid-induced oxidative damage. Therefore, we studied intramuscular lipid peroxide levels and high-fat diet induced alterations in muscle lipid metabolism of UCP3-ablated mice. UCP3(-/-) mice showed approximately 3-fold higher levels of intramuscular lipid peroxides upon standard chow feeding, compared to wild-type littermates. Remarkably, this difference was no longer apparent on the high-fat diet. However, upon high-fat feeding, intramuscular triacylglycerol levels were approximately 50% lower in UCP3(-/-) mice, in comparison to UCP3(+/+) animals. Succinate dehydrogenase activity, and total protein content of the muscle fatty acid transporter FAT/CD36 were however similar between UCP3(-/-) and UCP3(+/+) mice.
Hoeks, J., Hesselink, M. K., Sluiter, W., Schaart, G., Willems, J., Morrisson, A., Clapham, J. C., Saris, W. H., & Schrauwen, P. (2006). The effect of high-fat feeding on intramuscular lipid and lipid peroxidation levels in UCP3-ablated mice. Febs Letters, 580(5), 1371-1375. https://doi.org/10.1016/j.febslet.2006.01.059