The effect of high-fat feeding on intramuscular lipid and lipid peroxidation levels in UCP3-ablated mice

J. Hoeks, M.K. Hesselink, W. Sluiter, G. Schaart, J. Willems, A. Morrisson, J.C. Clapham, W.H. Saris, P. Schrauwen

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Abstract

Uncoupling protein-3 (UCP3) has been suggested to protect against lipid-induced oxidative damage. Therefore, we studied intramuscular lipid peroxide levels and high-fat diet induced alterations in muscle lipid metabolism of UCP3-ablated mice. UCP3(-/-) mice showed approximately 3-fold higher levels of intramuscular lipid peroxides upon standard chow feeding, compared to wild-type littermates. Remarkably, this difference was no longer apparent on the high-fat diet. However, upon high-fat feeding, intramuscular triacylglycerol levels were approximately 50% lower in UCP3(-/-) mice, in comparison to UCP3(+/+) animals. Succinate dehydrogenase activity, and total protein content of the muscle fatty acid transporter FAT/CD36 were however similar between UCP3(-/-) and UCP3(+/+) mice.
Original languageEnglish
Pages (from-to)1371-1375
JournalFebs Letters
Volume580
Issue number5
DOIs
Publication statusPublished - 1 Jan 2006

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