The effect of a 3-month low-intensity endurance training program on fat oxidation and acetyl-CoA carboxylase-2 expression

P. Schrauwen, D.P.C. van Leijssen - Aggel, G.B.J. Hul, A.J.M. Wagenmakers, H. Vidal, W.H.M. Saris, M.A. van Baak

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Abstract

Department of Human Biology, Nutrition, Toxicology and Environmental Research Institute (NUTRIM), Maastricht University, PO Box 616, 6200 MD Maastricht, the Netherlands. p.schrauwen@hb.unimaas.nl

Endurance training has been shown to increase fat oxidation both at rest and during exercise. However, most exercise training studies have been performed at high exercise intensity in well-trained athletes, and not much is known about the effect of a low-intensity training program on fat oxidation capacity in lean sedentary humans. Here, we examine the effect of 3-month low-intensity training program on total and intramuscular triglyceride (IMTG)- and/or VLDL-derived fat oxidation capacity and skeletal muscle mRNA expression. Six healthy untrained subjects (aged 43 +/- 2 years, BMI 22.7 +/- 1.1 kg/ m(2), V(O)(2max) 3.2 +/- 0.2 l/min) participated in a supervised 12-week training program at 40% V(O)(2max) three times weekly. Total and plasma-derived fatty acid oxidation at rest and during 1 h exercise was measured using [(13)C]palmitate, and in a separate test, [(13)C]acetate recovery was determined. Muscle biopsies were taken after an overnight fast. Total fat oxidation during exercise increased from 1,241 +/- 93 to 1,591 +/- 130 micromol/min (P = 0.06), and IMTG- and/or VLDL-derived fatty acid oxidation increased from 236 +/- 84 to 639 +/- 172 micromol/min (P = 0.09). Acetyl-CoA carboxylase-2 mRNA expression was significantly decreased after training (P = 0.005), whereas lipoprotein lipase mRNA expression tended to increase (P = 0.07). In conclusion, a minimal amount of physical activity tends to increase fat oxidation and leads to marked changes in the expression of genes encoding for key enzymes in fat metabolism.
Original languageEnglish
Pages (from-to)2220-2226
Number of pages7
JournalDiabetes
Volume51
Issue number7
DOIs
Publication statusPublished - 1 Jan 2002

Cite this

Schrauwen, P., van Leijssen - Aggel, D. P. C., Hul, G. B. J., Wagenmakers, A. J. M., Vidal, H., Saris, W. H. M., & van Baak, M. A. (2002). The effect of a 3-month low-intensity endurance training program on fat oxidation and acetyl-CoA carboxylase-2 expression. Diabetes, 51(7), 2220-2226. https://doi.org/10.2337/diabetes.51.7.2220
Schrauwen, P. ; van Leijssen - Aggel, D.P.C. ; Hul, G.B.J. ; Wagenmakers, A.J.M. ; Vidal, H. ; Saris, W.H.M. ; van Baak, M.A. / The effect of a 3-month low-intensity endurance training program on fat oxidation and acetyl-CoA carboxylase-2 expression. In: Diabetes. 2002 ; Vol. 51, No. 7. pp. 2220-2226.
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abstract = "Department of Human Biology, Nutrition, Toxicology and Environmental Research Institute (NUTRIM), Maastricht University, PO Box 616, 6200 MD Maastricht, the Netherlands. p.schrauwen@hb.unimaas.nlEndurance training has been shown to increase fat oxidation both at rest and during exercise. However, most exercise training studies have been performed at high exercise intensity in well-trained athletes, and not much is known about the effect of a low-intensity training program on fat oxidation capacity in lean sedentary humans. Here, we examine the effect of 3-month low-intensity training program on total and intramuscular triglyceride (IMTG)- and/or VLDL-derived fat oxidation capacity and skeletal muscle mRNA expression. Six healthy untrained subjects (aged 43 +/- 2 years, BMI 22.7 +/- 1.1 kg/ m(2), V(O)(2max) 3.2 +/- 0.2 l/min) participated in a supervised 12-week training program at 40{\%} V(O)(2max) three times weekly. Total and plasma-derived fatty acid oxidation at rest and during 1 h exercise was measured using [(13)C]palmitate, and in a separate test, [(13)C]acetate recovery was determined. Muscle biopsies were taken after an overnight fast. Total fat oxidation during exercise increased from 1,241 +/- 93 to 1,591 +/- 130 micromol/min (P = 0.06), and IMTG- and/or VLDL-derived fatty acid oxidation increased from 236 +/- 84 to 639 +/- 172 micromol/min (P = 0.09). Acetyl-CoA carboxylase-2 mRNA expression was significantly decreased after training (P = 0.005), whereas lipoprotein lipase mRNA expression tended to increase (P = 0.07). In conclusion, a minimal amount of physical activity tends to increase fat oxidation and leads to marked changes in the expression of genes encoding for key enzymes in fat metabolism.",
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The effect of a 3-month low-intensity endurance training program on fat oxidation and acetyl-CoA carboxylase-2 expression. / Schrauwen, P.; van Leijssen - Aggel, D.P.C.; Hul, G.B.J.; Wagenmakers, A.J.M.; Vidal, H.; Saris, W.H.M.; van Baak, M.A.

In: Diabetes, Vol. 51, No. 7, 01.01.2002, p. 2220-2226.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - The effect of a 3-month low-intensity endurance training program on fat oxidation and acetyl-CoA carboxylase-2 expression

AU - Schrauwen, P.

AU - van Leijssen - Aggel, D.P.C.

AU - Hul, G.B.J.

AU - Wagenmakers, A.J.M.

AU - Vidal, H.

AU - Saris, W.H.M.

AU - van Baak, M.A.

PY - 2002/1/1

Y1 - 2002/1/1

N2 - Department of Human Biology, Nutrition, Toxicology and Environmental Research Institute (NUTRIM), Maastricht University, PO Box 616, 6200 MD Maastricht, the Netherlands. p.schrauwen@hb.unimaas.nlEndurance training has been shown to increase fat oxidation both at rest and during exercise. However, most exercise training studies have been performed at high exercise intensity in well-trained athletes, and not much is known about the effect of a low-intensity training program on fat oxidation capacity in lean sedentary humans. Here, we examine the effect of 3-month low-intensity training program on total and intramuscular triglyceride (IMTG)- and/or VLDL-derived fat oxidation capacity and skeletal muscle mRNA expression. Six healthy untrained subjects (aged 43 +/- 2 years, BMI 22.7 +/- 1.1 kg/ m(2), V(O)(2max) 3.2 +/- 0.2 l/min) participated in a supervised 12-week training program at 40% V(O)(2max) three times weekly. Total and plasma-derived fatty acid oxidation at rest and during 1 h exercise was measured using [(13)C]palmitate, and in a separate test, [(13)C]acetate recovery was determined. Muscle biopsies were taken after an overnight fast. Total fat oxidation during exercise increased from 1,241 +/- 93 to 1,591 +/- 130 micromol/min (P = 0.06), and IMTG- and/or VLDL-derived fatty acid oxidation increased from 236 +/- 84 to 639 +/- 172 micromol/min (P = 0.09). Acetyl-CoA carboxylase-2 mRNA expression was significantly decreased after training (P = 0.005), whereas lipoprotein lipase mRNA expression tended to increase (P = 0.07). In conclusion, a minimal amount of physical activity tends to increase fat oxidation and leads to marked changes in the expression of genes encoding for key enzymes in fat metabolism.

AB - Department of Human Biology, Nutrition, Toxicology and Environmental Research Institute (NUTRIM), Maastricht University, PO Box 616, 6200 MD Maastricht, the Netherlands. p.schrauwen@hb.unimaas.nlEndurance training has been shown to increase fat oxidation both at rest and during exercise. However, most exercise training studies have been performed at high exercise intensity in well-trained athletes, and not much is known about the effect of a low-intensity training program on fat oxidation capacity in lean sedentary humans. Here, we examine the effect of 3-month low-intensity training program on total and intramuscular triglyceride (IMTG)- and/or VLDL-derived fat oxidation capacity and skeletal muscle mRNA expression. Six healthy untrained subjects (aged 43 +/- 2 years, BMI 22.7 +/- 1.1 kg/ m(2), V(O)(2max) 3.2 +/- 0.2 l/min) participated in a supervised 12-week training program at 40% V(O)(2max) three times weekly. Total and plasma-derived fatty acid oxidation at rest and during 1 h exercise was measured using [(13)C]palmitate, and in a separate test, [(13)C]acetate recovery was determined. Muscle biopsies were taken after an overnight fast. Total fat oxidation during exercise increased from 1,241 +/- 93 to 1,591 +/- 130 micromol/min (P = 0.06), and IMTG- and/or VLDL-derived fatty acid oxidation increased from 236 +/- 84 to 639 +/- 172 micromol/min (P = 0.09). Acetyl-CoA carboxylase-2 mRNA expression was significantly decreased after training (P = 0.005), whereas lipoprotein lipase mRNA expression tended to increase (P = 0.07). In conclusion, a minimal amount of physical activity tends to increase fat oxidation and leads to marked changes in the expression of genes encoding for key enzymes in fat metabolism.

U2 - 10.2337/diabetes.51.7.2220

DO - 10.2337/diabetes.51.7.2220

M3 - Article

VL - 51

SP - 2220

EP - 2226

JO - Diabetes

JF - Diabetes

SN - 0012-1797

IS - 7

ER -