The differential plasma proteome of obese and overweight individuals undergoing a nutritional weight loss and maintenance intervention

Sergio Moreno, Ornella Cominetti, Antonio Nunez Galindo, Irina Irincheeva, John Corthesy, Arne Astrup, Wimh. M. Saris, Jorg Hager, Martin Kussmann, Loic Dayon*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

25 Citations (Web of Science)

Abstract

Purpose: The nutritional intervention program DiOGenes focuses on how obesity can be prevented and treated from a dietary perspective. We generated differential plasma proteome profiles in the DiOGenes cohort to identify proteins associated with weight loss and maintenance and explore their relation to body mass index, fat mass, insulin resistance, and sensitivity. Experimental design: Relative protein quantification was obtained at baseline and after combined weight loss/maintenance phases using isobaric tagging and MS/MS. A Welch t-test determined proteins differentially present after intervention. Protein relationships with clinical variables were explored using univariate linear models, considering collection center, gender and age as confounding factors. Results: Four hundred and seventy three subjects were measured at baseline and end of the intervention; 39 proteins were longitudinally differential. Proteins with largest changes were sex hormone-binding globulin, adiponectin, C-reactive protein, calprotectin, serum amyloid A, and proteoglycan 4 (PRG4), whose association with obesity and weight loss is known. We identified new putative biomarkers for weight loss/maintenance. Correlation between PRG4 and proline-rich acidic protein 1 variation and Matsuda insulin sensitivity increment was showed. Conclusion and clinical relevance: MS-based proteomic analysis of a large cohort of non-diabetic overweight and obese individuals concomitantly identified known and novel proteins associated with weight loss and maintenance.
Original languageEnglish
Article number1600150
Number of pages11
JournalProteomics Clinical Applications
Volume12
Issue number1
DOIs
Publication statusPublished - 1 Jan 2018

Keywords

  • Biomarker
  • Diabetes
  • Large-scale study
  • Mass spectrometry
  • Obesity
  • SERUM AMYLOID-A
  • ADIPOSE-TISSUE
  • BIOMARKER DISCOVERY
  • PREMENOPAUSAL WOMEN
  • BINDING GLOBULIN
  • GLYCEMIC INDEX
  • INSULIN
  • GALECTIN-3
  • DIET
  • CALPROTECTIN

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