The Actin Regulator Coronin-1A Modulates Platelet Shape Change and Consolidates Arterial Thrombosis

Thomas J. Stocker; Joachim Pircher; Artid Skenderi; Andreas Ehrlich; Clemens Eberle; Remco T. A. Megens; Tobias Petzold; Zhe Zhang; Barbara Walzog; Annette Mueller-Taubenberger; Christian Weber; Steffen Massberg; Hellen Ishikawa-Ankerhold; Christian Schulz

doi:10.1055/s-0038-1675604

The Actin Regulator Coronin-1A Modulates Platelet Shape Change and Consolidates Arterial Thrombosis

Thomas J. Stocker^*, Joachim Pircher, Artid Skenderi, Andreas Ehrlich, Clemens Eberle, Remco T. A. Megens, Tobias Petzold, Zhe Zhang, Barbara Walzog, Annette Mueller-Taubenberger, Christian Weber, Steffen Massberg, Hellen Ishikawa-Ankerhold, Christian Schulz^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

7 Citations (Web of Science)

Abstract

Coronin-1A (Coro1A) belongs to a family of highly conserved actin-binding proteins that regulate cytoskeletal re-arrangement. In mammalians, Coro1A expression is most abundant in the haematopoietic lineage, where it regulates various cellular processes. The role of Coro1A in platelets has been previously unknown. Here, we identified Coro1A in human and mouse platelets. Genetic absence of Coro1A in mouse platelets inhibited agonist-induced actin polymerization and altered cofilin phosphoregulation, leading to a reduction in spreading and low-dose collagen induced aggregation. Furthermore, Coro1A-deficient mice displayed a defect in ferric chloride-induced arterial thrombosis with prolonged thrombus formation and reduced thrombus size. Immunofluorescence analysis revealed a less compact thrombus structure with reduced density of platelets and fibrinogen. In summary, Coro1A has a role in platelet biology with impact on spreading, aggregation and thrombosis.

Original language	English
Pages (from-to)	2098-2111
Number of pages	14
Journal	Thrombosis and Haemostasis
Volume	118
Issue number	12
DOIs	https://doi.org/10.1055/s-0038-1675604
Publication status	Published - Dec 2018

Keywords

coronin-1A
coro1A
platelets
thrombosis
actin
BINDING-PROTEIN
ARP2/3 COMPLEX
F-ACTIN
COFILIN
DYNAMICS
RAC1
PHAGOCYTOSIS
TRAFFICKING
NEUTROPHILS
CHEMOTAXIS

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10.1055/s-0038-1675604

Cite this

Stocker, T. J., Pircher, J., Skenderi, A., Ehrlich, A., Eberle, C., Megens, R. T. A., Petzold, T., Zhang, Z., Walzog, B., Mueller-Taubenberger, A., Weber, C., Massberg, S., Ishikawa-Ankerhold, H., & Schulz, C. (2018). The Actin Regulator Coronin-1A Modulates Platelet Shape Change and Consolidates Arterial Thrombosis. Thrombosis and Haemostasis, 118(12), 2098-2111. https://doi.org/10.1055/s-0038-1675604

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title = "The Actin Regulator Coronin-1A Modulates Platelet Shape Change and Consolidates Arterial Thrombosis",

abstract = "Coronin-1A (Coro1A) belongs to a family of highly conserved actin-binding proteins that regulate cytoskeletal re-arrangement. In mammalians, Coro1A expression is most abundant in the haematopoietic lineage, where it regulates various cellular processes. The role of Coro1A in platelets has been previously unknown. Here, we identified Coro1A in human and mouse platelets. Genetic absence of Coro1A in mouse platelets inhibited agonist-induced actin polymerization and altered cofilin phosphoregulation, leading to a reduction in spreading and low-dose collagen induced aggregation. Furthermore, Coro1A-deficient mice displayed a defect in ferric chloride-induced arterial thrombosis with prolonged thrombus formation and reduced thrombus size. Immunofluorescence analysis revealed a less compact thrombus structure with reduced density of platelets and fibrinogen. In summary, Coro1A has a role in platelet biology with impact on spreading, aggregation and thrombosis.",

keywords = "coronin-1A, coro1A, platelets, thrombosis, actin, BINDING-PROTEIN, ARP2/3 COMPLEX, F-ACTIN, COFILIN, DYNAMICS, RAC1, PHAGOCYTOSIS, TRAFFICKING, NEUTROPHILS, CHEMOTAXIS",

author = "Stocker, {Thomas J.} and Joachim Pircher and Artid Skenderi and Andreas Ehrlich and Clemens Eberle and Megens, {Remco T. A.} and Tobias Petzold and Zhe Zhang and Barbara Walzog and Annette Mueller-Taubenberger and Christian Weber and Steffen Massberg and Hellen Ishikawa-Ankerhold and Christian Schulz",

year = "2018",

month = dec,

doi = "10.1055/s-0038-1675604",

language = "English",

volume = "118",

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Stocker, TJ, Pircher, J, Skenderi, A, Ehrlich, A, Eberle, C, Megens, RTA, Petzold, T, Zhang, Z, Walzog, B, Mueller-Taubenberger, A, Weber, C, Massberg, S, Ishikawa-Ankerhold, H & Schulz, C 2018, 'The Actin Regulator Coronin-1A Modulates Platelet Shape Change and Consolidates Arterial Thrombosis', Thrombosis and Haemostasis, vol. 118, no. 12, pp. 2098-2111. https://doi.org/10.1055/s-0038-1675604

TY - JOUR

T1 - The Actin Regulator Coronin-1A Modulates Platelet Shape Change and Consolidates Arterial Thrombosis

AU - Stocker, Thomas J.

AU - Pircher, Joachim

AU - Skenderi, Artid

AU - Ehrlich, Andreas

AU - Eberle, Clemens

AU - Megens, Remco T. A.

AU - Petzold, Tobias

AU - Zhang, Zhe

AU - Walzog, Barbara

AU - Mueller-Taubenberger, Annette

AU - Weber, Christian

AU - Massberg, Steffen

AU - Ishikawa-Ankerhold, Hellen

AU - Schulz, Christian

PY - 2018/12

Y1 - 2018/12

N2 - Coronin-1A (Coro1A) belongs to a family of highly conserved actin-binding proteins that regulate cytoskeletal re-arrangement. In mammalians, Coro1A expression is most abundant in the haematopoietic lineage, where it regulates various cellular processes. The role of Coro1A in platelets has been previously unknown. Here, we identified Coro1A in human and mouse platelets. Genetic absence of Coro1A in mouse platelets inhibited agonist-induced actin polymerization and altered cofilin phosphoregulation, leading to a reduction in spreading and low-dose collagen induced aggregation. Furthermore, Coro1A-deficient mice displayed a defect in ferric chloride-induced arterial thrombosis with prolonged thrombus formation and reduced thrombus size. Immunofluorescence analysis revealed a less compact thrombus structure with reduced density of platelets and fibrinogen. In summary, Coro1A has a role in platelet biology with impact on spreading, aggregation and thrombosis.

AB - Coronin-1A (Coro1A) belongs to a family of highly conserved actin-binding proteins that regulate cytoskeletal re-arrangement. In mammalians, Coro1A expression is most abundant in the haematopoietic lineage, where it regulates various cellular processes. The role of Coro1A in platelets has been previously unknown. Here, we identified Coro1A in human and mouse platelets. Genetic absence of Coro1A in mouse platelets inhibited agonist-induced actin polymerization and altered cofilin phosphoregulation, leading to a reduction in spreading and low-dose collagen induced aggregation. Furthermore, Coro1A-deficient mice displayed a defect in ferric chloride-induced arterial thrombosis with prolonged thrombus formation and reduced thrombus size. Immunofluorescence analysis revealed a less compact thrombus structure with reduced density of platelets and fibrinogen. In summary, Coro1A has a role in platelet biology with impact on spreading, aggregation and thrombosis.

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KW - coro1A

KW - platelets

KW - thrombosis

KW - actin

KW - BINDING-PROTEIN

KW - ARP2/3 COMPLEX

KW - F-ACTIN

KW - COFILIN

KW - DYNAMICS

KW - RAC1

KW - PHAGOCYTOSIS

KW - TRAFFICKING

KW - NEUTROPHILS

KW - CHEMOTAXIS

U2 - 10.1055/s-0038-1675604

DO - 10.1055/s-0038-1675604

M3 - Article

C2 - 30453345

SN - 0340-6245

VL - 118

SP - 2098

EP - 2111

JO - Thrombosis and Haemostasis

JF - Thrombosis and Haemostasis

IS - 12

ER -