TFAP2B Influences the Effect of Dietary Fat on Weight Loss under Energy Restriction

T. Stocks; L. Angquist; K. Banasik; M. N. Harder; M.A. Taylor; J. Hager; P. Arner; J.M. Oppert; J.A. Martinez; J. Polak; F. Rousseau; D. Langin; S. Rossner; C. Holst; I.A. Macdonald; Y. Kamatani; A.F. Pfeiffer; M. Kunesova; W.H. Saris; T. Hansen; O. Pedersen; A. Astrup; T.I. Sorensen

doi:10.1371/journal.pone.0043212

TFAP2B Influences the Effect of Dietary Fat on Weight Loss under Energy Restriction

T. Stocks^*, L. Angquist, K. Banasik, M. N. Harder, M.A. Taylor, J. Hager, P. Arner, J.M. Oppert, J.A. Martinez, J. Polak, F. Rousseau, D. Langin, S. Rossner, C. Holst, I.A. Macdonald, Y. Kamatani, A.F. Pfeiffer, M. Kunesova, W.H. Saris, T. HansenO. Pedersen, A. Astrup, T.I. Sorensen

^*Corresponding author for this work

Humane Biologie

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

BACKGROUND: Numerous gene loci are related to single measures of body weight and shape. We investigated if 55 SNPs previously associated with BMI or waist measures, modify the effects of fat intake on weight loss and waist reduction under energy restriction. METHODS AND FINDINGS: Randomized controlled trial of 771 obese adults. (Registration: ISRCTN25867281.) One SNP was selected for replication in another weight loss intervention study of 934 obese adults. The original trial was a 10-week 600 kcal/d energy-deficient diet with energy percentage from fat (fat%) in range of 20-25 or 40-45. The replication study used an 8-weeks diet of 880 kcal/d and 20 fat%; change in fat% intake was used for estimation of interaction effects. The main outcomes were intervention weight loss and waist reduction. In the trial, mean change in fat% intake was -12/+4 in the low/high-fat groups. In the replication study, it was -23/-12 among those reducing fat% more/less than the median. TFAP2B-rs987237 genotype AA was associated with 1.0 kg (95% CI, 0.4; 1.6) greater weight loss on the low-fat, and GG genotype with 2.6 kg (1.1; 4.1) greater weight loss on the high-fat (interaction p-value; p = 0.00007). The replication study showed a similar (non-significant) interaction pattern. Waist reduction results generally were similar. Study-strengths include (i) the discovery study randomised trial design combined with the replication opportunity (ii) the strict dietary intake control in both studies (iii) the large sample sizes of both studies. Limitations are (i) the low minor allele frequency of the TFAP2B polymorphism, making it hard to investigate non-additive genetic effects (ii) the different interventions preventing identical replication-discovery study designs (iii) some missing data for non-completers and dietary intake. No adverse effects/outcomes or side-effects were observed. CONCLUSIONS: Under energy restriction, TFAP2B may modify the effect of dietary fat intake on weight loss and waist reduction.

Original language	English
Article number	43212
Number of pages	10
Journal	PLOS ONE
Volume	7
Issue number	8
DOIs	https://doi.org/10.1371/journal.pone.0043212
Publication status	Published - 27 Aug 2012

Keywords

GENOME-WIDE ASSOCIATION
OBESITY-RELATED TRAITS
BODY-MASS INDEX
LOW-CARBOHYDRATE
FTO GENE
INSULIN-RESISTANCE
RANDOMIZED-TRIAL
IDENTICAL-TWINS
ADULT OBESITY
LOCI

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Cite this

Stocks, T., Angquist, L., Banasik, K., Harder, M. N., Taylor, M. A., Hager, J., Arner, P., Oppert, J. M., Martinez, J. A., Polak, J., Rousseau, F., Langin, D., Rossner, S., Holst, C., Macdonald, I. A., Kamatani, Y., Pfeiffer, A. F., Kunesova, M., Saris, W. H., ... Sorensen, T. I. (2012). TFAP2B Influences the Effect of Dietary Fat on Weight Loss under Energy Restriction. PLOS ONE, 7(8), Article 43212. https://doi.org/10.1371/journal.pone.0043212

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title = "TFAP2B Influences the Effect of Dietary Fat on Weight Loss under Energy Restriction",

abstract = "BACKGROUND: Numerous gene loci are related to single measures of body weight and shape. We investigated if 55 SNPs previously associated with BMI or waist measures, modify the effects of fat intake on weight loss and waist reduction under energy restriction. METHODS AND FINDINGS: Randomized controlled trial of 771 obese adults. (Registration: ISRCTN25867281.) One SNP was selected for replication in another weight loss intervention study of 934 obese adults. The original trial was a 10-week 600 kcal/d energy-deficient diet with energy percentage from fat (fat%) in range of 20-25 or 40-45. The replication study used an 8-weeks diet of 880 kcal/d and 20 fat%; change in fat% intake was used for estimation of interaction effects. The main outcomes were intervention weight loss and waist reduction. In the trial, mean change in fat% intake was -12/+4 in the low/high-fat groups. In the replication study, it was -23/-12 among those reducing fat% more/less than the median. TFAP2B-rs987237 genotype AA was associated with 1.0 kg (95% CI, 0.4; 1.6) greater weight loss on the low-fat, and GG genotype with 2.6 kg (1.1; 4.1) greater weight loss on the high-fat (interaction p-value; p = 0.00007). The replication study showed a similar (non-significant) interaction pattern. Waist reduction results generally were similar. Study-strengths include (i) the discovery study randomised trial design combined with the replication opportunity (ii) the strict dietary intake control in both studies (iii) the large sample sizes of both studies. Limitations are (i) the low minor allele frequency of the TFAP2B polymorphism, making it hard to investigate non-additive genetic effects (ii) the different interventions preventing identical replication-discovery study designs (iii) some missing data for non-completers and dietary intake. No adverse effects/outcomes or side-effects were observed. CONCLUSIONS: Under energy restriction, TFAP2B may modify the effect of dietary fat intake on weight loss and waist reduction.",

keywords = "GENOME-WIDE ASSOCIATION, OBESITY-RELATED TRAITS, BODY-MASS INDEX, LOW-CARBOHYDRATE, FTO GENE, INSULIN-RESISTANCE, RANDOMIZED-TRIAL, IDENTICAL-TWINS, ADULT OBESITY, LOCI",

author = "T. Stocks and L. Angquist and K. Banasik and Harder, {M. N.} and M.A. Taylor and J. Hager and P. Arner and J.M. Oppert and J.A. Martinez and J. Polak and F. Rousseau and D. Langin and S. Rossner and C. Holst and I.A. Macdonald and Y. Kamatani and A.F. Pfeiffer and M. Kunesova and W.H. Saris and T. Hansen and O. Pedersen and A. Astrup and T.I. Sorensen",

year = "2012",

month = aug,

day = "27",

doi = "10.1371/journal.pone.0043212",

language = "English",

volume = "7",

journal = "PLOS ONE",

issn = "1932-6203",

publisher = "Public Library of Science",

number = "8",

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Stocks, T, Angquist, L, Banasik, K, Harder, MN, Taylor, MA, Hager, J, Arner, P, Oppert, JM, Martinez, JA, Polak, J, Rousseau, F, Langin, D, Rossner, S, Holst, C, Macdonald, IA, Kamatani, Y, Pfeiffer, AF, Kunesova, M, Saris, WH, Hansen, T, Pedersen, O, Astrup, A & Sorensen, TI 2012, 'TFAP2B Influences the Effect of Dietary Fat on Weight Loss under Energy Restriction', PLOS ONE, vol. 7, no. 8, 43212. https://doi.org/10.1371/journal.pone.0043212

TY - JOUR

T1 - TFAP2B Influences the Effect of Dietary Fat on Weight Loss under Energy Restriction

AU - Stocks, T.

AU - Angquist, L.

AU - Banasik, K.

AU - Harder, M. N.

AU - Taylor, M.A.

AU - Hager, J.

AU - Arner, P.

AU - Oppert, J.M.

AU - Martinez, J.A.

AU - Polak, J.

AU - Rousseau, F.

AU - Langin, D.

AU - Rossner, S.

AU - Holst, C.

AU - Macdonald, I.A.

AU - Kamatani, Y.

AU - Pfeiffer, A.F.

AU - Kunesova, M.

AU - Saris, W.H.

AU - Hansen, T.

AU - Pedersen, O.

AU - Astrup, A.

AU - Sorensen, T.I.

PY - 2012/8/27

Y1 - 2012/8/27

N2 - BACKGROUND: Numerous gene loci are related to single measures of body weight and shape. We investigated if 55 SNPs previously associated with BMI or waist measures, modify the effects of fat intake on weight loss and waist reduction under energy restriction. METHODS AND FINDINGS: Randomized controlled trial of 771 obese adults. (Registration: ISRCTN25867281.) One SNP was selected for replication in another weight loss intervention study of 934 obese adults. The original trial was a 10-week 600 kcal/d energy-deficient diet with energy percentage from fat (fat%) in range of 20-25 or 40-45. The replication study used an 8-weeks diet of 880 kcal/d and 20 fat%; change in fat% intake was used for estimation of interaction effects. The main outcomes were intervention weight loss and waist reduction. In the trial, mean change in fat% intake was -12/+4 in the low/high-fat groups. In the replication study, it was -23/-12 among those reducing fat% more/less than the median. TFAP2B-rs987237 genotype AA was associated with 1.0 kg (95% CI, 0.4; 1.6) greater weight loss on the low-fat, and GG genotype with 2.6 kg (1.1; 4.1) greater weight loss on the high-fat (interaction p-value; p = 0.00007). The replication study showed a similar (non-significant) interaction pattern. Waist reduction results generally were similar. Study-strengths include (i) the discovery study randomised trial design combined with the replication opportunity (ii) the strict dietary intake control in both studies (iii) the large sample sizes of both studies. Limitations are (i) the low minor allele frequency of the TFAP2B polymorphism, making it hard to investigate non-additive genetic effects (ii) the different interventions preventing identical replication-discovery study designs (iii) some missing data for non-completers and dietary intake. No adverse effects/outcomes or side-effects were observed. CONCLUSIONS: Under energy restriction, TFAP2B may modify the effect of dietary fat intake on weight loss and waist reduction.

AB - BACKGROUND: Numerous gene loci are related to single measures of body weight and shape. We investigated if 55 SNPs previously associated with BMI or waist measures, modify the effects of fat intake on weight loss and waist reduction under energy restriction. METHODS AND FINDINGS: Randomized controlled trial of 771 obese adults. (Registration: ISRCTN25867281.) One SNP was selected for replication in another weight loss intervention study of 934 obese adults. The original trial was a 10-week 600 kcal/d energy-deficient diet with energy percentage from fat (fat%) in range of 20-25 or 40-45. The replication study used an 8-weeks diet of 880 kcal/d and 20 fat%; change in fat% intake was used for estimation of interaction effects. The main outcomes were intervention weight loss and waist reduction. In the trial, mean change in fat% intake was -12/+4 in the low/high-fat groups. In the replication study, it was -23/-12 among those reducing fat% more/less than the median. TFAP2B-rs987237 genotype AA was associated with 1.0 kg (95% CI, 0.4; 1.6) greater weight loss on the low-fat, and GG genotype with 2.6 kg (1.1; 4.1) greater weight loss on the high-fat (interaction p-value; p = 0.00007). The replication study showed a similar (non-significant) interaction pattern. Waist reduction results generally were similar. Study-strengths include (i) the discovery study randomised trial design combined with the replication opportunity (ii) the strict dietary intake control in both studies (iii) the large sample sizes of both studies. Limitations are (i) the low minor allele frequency of the TFAP2B polymorphism, making it hard to investigate non-additive genetic effects (ii) the different interventions preventing identical replication-discovery study designs (iii) some missing data for non-completers and dietary intake. No adverse effects/outcomes or side-effects were observed. CONCLUSIONS: Under energy restriction, TFAP2B may modify the effect of dietary fat intake on weight loss and waist reduction.

KW - GENOME-WIDE ASSOCIATION

KW - OBESITY-RELATED TRAITS

KW - BODY-MASS INDEX

KW - LOW-CARBOHYDRATE

KW - FTO GENE

KW - INSULIN-RESISTANCE

KW - RANDOMIZED-TRIAL

KW - IDENTICAL-TWINS

KW - ADULT OBESITY

KW - LOCI

U2 - 10.1371/journal.pone.0043212

DO - 10.1371/journal.pone.0043212

M3 - Article

SN - 1932-6203

VL - 7

JO - PLOS ONE

JF - PLOS ONE

IS - 8

M1 - 43212

ER -